From Bioblast
O2k-Publications: Instruments;methods
- This topic relates to methodological publications on bioenergetics.
Sort in ascending/descending order by a click on one of the small symbols in squares below. Default sorting: chronological. Empty fields appear first in ascending order.
Year | Reference | |
---|---|---|
MiPNet28.07 NextGen-O2k Series XB manual | 2023-10-19 | NextGen-O2k Series XB manual |
MiPNet14.06 Instrumental O2 background | 2023-10-19 | O2k Quality Control 2: Instrumental oxygen background correction and accuracy of oxygen flux. |
MiPNet28.08 Oroboros O2k Series J manual | 2023-10-19 | Oroboros O2k Series J manual |
MiPNet28.10 SmartPOS-service | 2023-09-29 | SmartPOS: Service |
MiPNet19.14 SOP Hamilton microsyringes | 2023-09-28 | SOP for manual O2k-titrations with Hamilton microsyringes. |
MiPNet28.09 O2k-Fluo Smart-Module manual | 2023-09-25 | O2k-Fluo Smart-Module manual |
MiPNet19.03 O2k-cleaning and ISS | 2023-03-14 | O2k-Chamber cleaning SOP and Integrated Suction System (ISS). |
MiPNet22.11 O2k-FluoRespirometer manual | 2023-03-14 | O2k-FluoRespirometer manual. |
MiPNet06.03 POS-calibration-SOP | 2023-02-06 | O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration. |
Ebanks 2023 PLoS One | 2023 | Ebanks B, Kwiecinska P, Moisoi N, Chakrabarti L (2023) A method to assess the mitochondrial respiratory capacity of complexes I and II from frozen tissue using the Oroboros O2k-FluoRespirometer. https://doi.org/10.1371/journal.pone.0276147 |
Nord 2023 FASEB J | 2023 | Nord A, Chamkha I, Elmér E (2023) A whole blood approach improves speed and accuracy when measuring mitochondrial respiration in intact avian blood cells. https://doi.org/10.1096/fj.202201749r |
Mu 2023 STAR Protoc | 2023 | Mu C, Shearer J (2023) Protocol for measuring respiratory function of mitochondria in frozen colon tissue from rats. https://doi.org/10.1016/j.xpro.2023.102560 |
Giovarelli 2023 Mol Med | 2023 | Giovarelli M, Serati A, Zecchini S, Guelfi F, Clementi E, MandĂČ C (2023) Cryopreserved placental biopsies maintain mitochondrial activity for high-resolution respirometry. https://doi.org/10.1186/s10020-023-00645-2 |
MiPNet26.14 Oxia | 2022-10-31 | Oxia - HyperOxia to HypOxia. |
MiPNet27.07 Sample Holder | 2022-08-09 | Sample Holder. |
MiPNet26.13 NextGen-O2k manual | 2022-07-25 | NextGen-O2k manual |
MiPNet26.12 NextGen-O2k: NADH-Module | 2022-05-17 | NextGen-O2k: NADH-Module manual |
Baglivo 2022 BEC | 2022 | Baglivo E, Cardoso LHD, Cecatto C, Gnaiger E (2022) Statistical analysis of instrumental reproducibility as internal quality control in high-resolution respirometry. Bioenerg Commun 2022.8. https://doi.org/10.26124/bec:2022-0008 |
Crowder 2022 Islets | 2022 | Crowder JJ, Zeng Z, Novak AN, Alves NJ, Linnemann AK (2022) Stabilization protects islet integrity during respirometry in the Oroboros Oxygraph-2K analyzer. Islets 14:128-38. |
Komlodi 2022 BEC | 2022 | KomlĂłdi T, Tretter L (2022) The protonmotive force â not merely membrane potential. Bioenerg Commun 2022.16. https://doi.org/10.26124/bec:2022-0016 |
Baglivo 2022 MitoFit-QC | 2022 | Baglivo E, Cardoso LHD, Cecatto C, Gnaiger E (2022) Statistical analysis of instrumental reproducibility as internal quality control in high-resolution respirometry. https://doi.org/10.26124/mitofit:2022-0018.v2 â 2022-08-04 published in Bioenerg Commun 2022.8. |
Hansen 2022 Magn Reson Med | 2022 | Hansen K, Hansen ESS, Jespersen NRV, BĂžtker HE, Pedersen M, Wang T, Laustsen C (2022) Hyperpolarized 13C MRI reveals large changes in pyruvate metabolism during digestion in snakes. https://doi.org/10.1002/mrm.29239 |
Meza-Buendia 2022 PLoS One | 2022 | Meza-Buendia AK, Aparicio-Trejo OE, DĂaz F, Caamal-Monsreal C, Pedraza-Chaverri J, Ălvarez-Delgado C, Paschke K, Rosas C (2022) High resolution respirometry of isolated mitochondria from adult Octopus maya (Class: Cephalopoda) systemic heart. https://doi.org/10.1371/journal.pone.0273554 |
Serna 2022 MitoFit | 2022 | Serna JDC, Ramos VM, Cabral-Costa JV, Vilas-Boas EA, Amaral AG, Ohya G, da Silva CCC, Kowaltowski AJ (2022) Measuring mitochondrial Ca2+ efflux in isolated mitochondria and permeabilized cells. https://doi.org/10.26124/mitofit:2022-0021 â 2022-07-28 published in Bioenerg Commun 2022.7. |
Mihajlovic 2022 Int J Mol Sci | 2022 | Mihajlovic M, Vinken M (2022) Mitochondria as the target of hepatotoxicity and drug-induced liver injury: molecular mechanisms and detection methods. https://doi.org/10.3390/ijms23063315 |
Horwath 2022 J Physiol | 2022 | Horwath O, Edman S, Andersson A, Larsen FJ, AprĂł W (2022) THRIFTY: a novel high-throughput method for rapid fibre type identification of isolated skeletal muscle fibres. https://doi.org/10.1113/jp282959 |
Goudie 2022 Sci Rep | 2022 | Goudie L, Mancini NL, Shutt TE, Holloway GP, Mu C, Wang A, McKay DM, Shearer J (2022) Impact of experimental colitis on mitochondrial bioenergetics in intestinal epithelial cells. https://doi.org/10.1038/s41598-022-11123-w |
Pallag 2022 MitoFit Proline | 2022 | Pallag G, Nazarian S, Ravasz D, Bui D, KomlĂłdi T, Doerrier C, Gnaiger E, Seyfried TN, Chinopoulos C (2022) Proline oxidation leading to high electron flow through reduction of ubiquinone supports ATP production by F1FO-ATPase in mitochondria with inhibited Complex I. https://doi.org/10.26124/mitofit:2022-0001.v3 â 2022-05-04 published in https://doi.org/10.3390/ijms23095111 |
Formiga-Jr 2022 J Vis Exp | 2022 | Formiga-Jr MA, Camacho-Pereira J (2022) Assessing mitochondrial function in sciatic nerve by high-resolution respirometry. https://doi.org/10.3791/63690 |
Serna 2022 BEC | 2022 | Serna JDC, Ramos VM, Cabral-Costa JV, Vilas-Boas EA, Amaral AG, Ohya G, da Silva CCC, Kowaltowski AJ (2022) Measuring mitochondrial Ca2+ efflux in isolated mitochondria and permeabilized cells. https://doi.org/10.26124/bec:2022-0007 |
Zdrazilova 2022 PLOS ONE | 2022 | Zdrazilova L, Hansikova H, Gnaiger E (2022) Comparable respiratory activity in attached and suspended human fibroblasts. https://doi.org/10.1371/journal.pone.0264496 |
Mas-Bargues 2022 Antioxidants (Basel) | 2022 | Mas-Bargues C, GarcĂa-DomĂnguez E, BorrĂĄs C (2022) Recent approaches to determine static and dynamic redox state-related parameters. https://doi.org/10.3390/antiox11050864 |
MiPNet24.12 NextGen-O2k: Q-Module | 2021-10-29 | NextGen-O2k: Q-Module manual |
MiPNet26.11 NextGen-O2k: PB-Module | 2021-10-29 | NextGen-O2k: PB-Module manual |
MiPNet24.16 DatLab8.0: CV-Module | 2021-10-28 | DatLab8.0: Cyclic voltammetry manual |
MiPNet24.10 H2O2 flux analysis | 2021-10-22 | Hydrogen peroxide flux analysis using Amplex UltraRed assay in MiR05-Kit with DatLab 7.4 |
MiPNet24.09 Data analysis of mt-membrane potential | 2021-09-16 | Data analysis of mt-membrane potential. |
MiPNet26.10 MgG data analysis | 2021-09-16 | Measurement of mitochondrial ATP production using Magnesium Green: DL-Protocols and data analysis with DatLab 7.4 |
MiPNet15.03 O2k-MultiSensor-ISE | 2021-08-02 | O2k-MultiSensor system with ion selective electrodes (ISE). |
MiPNet12.10 TIP2k-manual | 2021-07-08 | Titration-Injection microPump TIP2k manual. |
MiPNet19.18B POS-service | 2021-06-23 | Service of the polarographic oxygen sensor OroboPOS. |
MiPNet17.05 O2k-Fluo LED2-Module | 2021-06-22 | O2k-Fluo LED2-Module. |
MiPNet26.06 DatLab 7: Guide | 2021-06-02 | DatLab-guide through the menus. |
MiPNet20.04 O2k-checklist | 2021-03-12 | O2k-checklist: get started with an O2k-experiment. |
Gnaiger 2021 MitoFit BCA | 2021 | Gnaiger E (2021) Bioenergetic cluster analysis â mitochondrial respiratory control in human fibroblasts. https://doi.org/10.26124/mitofit:2021-0008 |
Siewiera 2021 Int J Mol Sci | 2021 | Siewiera K, Labieniec-Watala M, Wolska N, Kassassir H, Watala C (2021) Sample preparation as a critical aspect of blood platelet mitochondrial respiration measurements - The impact of platelet activation on mitochondrial respiration. https://doi.org/10.3390/ijms22179332 |
Schmidt 2021 J Biol Chem | 2021 | Schmidt CA, Fisher-Wellman KH, Neufer PD (2021) From OCR and ECAR to energy: perspectives on the design and interpretation of bioenergetics studies. J Biol Chem 207:101140. https://doi.org/10.1016/j.jbc.2021.101140 |
Went 2021 MitoFit PB | 2021 | Went N, Di Marcello M, Gnaiger E (2021) Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry. https://doi.org/10.26124/mitofit:2021-0005 |
Komlodi 2021 BEC AmR-O2 | 2021 | KomlĂłdi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. https://doi.org/10.26124/bec:2021-0004 |
Jedlicka 2021 Physiol Res | 2021 | JedliÄka J, Kunc R, KuncovĂĄ J (2021) Mitochondrial respiration of human platelets in young adult and advanced age - Seahorse or O2k? Physiol Res 70:369-79. |
Komlodi 2021 BEC Q | 2021 | KomlĂłdi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. https://doi.org/10.26124/bec:2021-0003 |
Hoppel 2021 Cells | 2021 | Hoppel F, Garcia-Souza LF, Kantner-Rumplmair W, Burtscher M, Gnaiger E, Pesta D, Calabria E (2021) Human platelet mitochondrial function reflects systemic mitochondrial alterations: a protocol for application in field studies. https://doi.org/10.3390/cells10082088 |
Cardoso 2021 BEC MgG | 2021 | Cardoso LHD, Doerrier C, Gnaiger E (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. https://doi.org/10.26124/bec:2021-0001 |
Vernerova 2021 Biomedicines | 2021 | Vernerova A, Garcia-Souza LF, Soucek O, Kostal M, Rehacek V, Krcmova LK, Gnaiger E, Sobotka O (2021) Mitochondrial respiration of platelets: comparison of isolation methods. https://doi.org/10.3390/biomedicines9121859 |
MiPNet21.17 BloodCellsIsolation | 2020-10-22 | O2k-Protocols: Isolation of blood cells for HRR. |
MiPNet24.08 Safranin Analysis Template | 2020-05-13 | Excel template for safranin data analysis. |
MiPNet17.04 CitrateSynthase | 2020-04-18 | Laboratory protocol: Citrate synthase a mitochondrial marker enzyme. |
MiPNet24.06 Oxygen flux analysis - DatLab 7.4 | 2020-04-16 | Oxygen flux analysis with DatLab 7.4. |
MiPNet24.14 O2k-sV-Module manual | 2020-03-17 | O2k-sV-Module manual. |
MiPNet25.14 TPP Analysis Template | 2020-##-## | Excel template for TPP data analysis. |
Pola 2020 Invest New Drugs | 2020 | Pola M, Kolarova H, Ruzicka J, Zholobenko A, Modriansky M, Mosinger J, Bajgar R (2020) Effects of zinc porphyrin and zinc phthalocyanine derivatives in photodynamic anticancer therapy under different partial pressures of oxygen in vitro. Invest New Drugs 39:89-97. |
Fisher 2020 Placenta | 2020 | Fisher J, McKeating D, Pennell E, Cuffe J, Holland O, Perkins A (2020) Mitochondrial isolation, cryopreservation and preliminary biochemical characterisation from placental cytotrophoblast and syncytiotrophoblast. Placenta 82:1-4. |
Boehmert 2020 Transfusion | 2020 | Böhmert S, KĂŒbel S, MĂŒller MM, Weber CF, Adam EH, Dröse S, Zacharowski K, Fischer D (2020) The effect of the interruption of agitation, temporary cooling, and pneumatic tube transportation on platelet quality during storage for transfusion. Transfusion 61:1258-65. |
McCann 2020 Metabolites | 2020 | McCann MR, McHugh CE, Kirby M, Jennaro TS, Jones AE, Stringer KA, Puskarich MA (2020) A multivariate metabolomics method for estimating platelet mitochondrial oxygen consumption rates in patients with sepsis. Metabolites 10:E139. |
D'Urso 2020 Surg Endosc | 2020 | D'Urso A, Agnus V, Barberio M, Seeliger B, Marchegiani F, Charles AL, Geny B, Marescaux J, Mutter D, Diana M (2020) Computer-assisted quantification and visualization of bowel perfusion using fluorescence-based enhanced reality in left-sided colonic resections. Surg Endosc 35:4321-31. |
Dawid 2020 Biochim Biophys Acta Bioenerg | 2020 | Dawid C, Weber D, Musiol E, Janas V, Baur S, Lang R, Fromme T (2020) Comparative assessment of purified saponins as permeabilization agents during respirometry. Biochim Biophys Acta Bioenerg 1861:148251. |
Assis-de-Lemos 2020 Curr Protoc Toxicol | 2020 | Assis-de-Lemos G, Ledur PF, Karmirian K, Rehen SK, Galina A (2020) A protocol to study mitochondrial function in human neural progenitors and iPSC-derived astrocytes. Curr Protoc Toxicol 85:e97. |
Injarabian 2020 Sci Rep | 2020 | Injarabian L, Scherlinger M, Devin A, Ransac S, Lykkesfeldt J, Marteyn BS (2020) Ascorbate maintains a low plasma oxygen level. Sci Rep 10:10659. |
McLaughlin 2020b Sci Rep | 2020 | McLaughlin KL, Hagen JT, Coalson HS, Nelson MAM, Kew KA, Wooten AR, Fisher-Wellman KH (2020) Novel approach to quantify mitochondrial content and intrinsic bioenergetic efficiency across organs. Sci Rep 10:17599. |
Nolfi-Doneganab 2020 Redox Biol | 2020 | Nolfi-Doneganab D, Braganzaa A, Shivaac S (2020) Mitochondrial electron transport: oxidative phosphorylation, mitochondrial oxidant production, and methods of measurement. Redox Biol 2020:101674. |
Damiani 2020 Curr Opin Biotechnol | 2020 | Damiani C, Gaglio D, Sacco E, Alberghina L, Vanoni M (2020) Systems metabolomics: from metabolomic snapshots to design principles. Curr Opin Biotechnol 63:190-199. |
Huete-Ortega 2020 MitoFit Preprint Arch EA | 2020 | Huete-Ortega M, Di Marcello M, Iglesias-Gonzalez J, Gnaiger E (2020) High-resolution respirometry for chloroplast and mitochondrial bioenergetics in Chlamydomonas reinhardtii â towards biotechnology exploitations. https://doi.org/10.26124/mitofit:ea20.algaeurope.0001 |
Romeiro 2020 bioRxiv | 2020 | Romeiro NC, Ferreira CM, Oliveira MF (2020) Assessment of mitochondrial physiology of murine white adipose tissue by mechanical permeabilization and lipid depletion. bioRxiv doi: https://doi.org/10.1101/2020.02.19.956870. |
Madeo 2020 US Patent | 2020 | Madeo F, Eisenberg T, Stekovic S (2020) Use of spermidine for the enhancement of mitochondrial respiration. US Patent US20200054579 A1. |
Allen 2020 Thesis | 2020 | Allen FM (2020) Mitochondrial metabolism elucidated by rapid fractionation from tissue. PhD Thesis 298. |
Gnaiger 2020 BEC MitoPathways | 2020 | Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. https://doi.org/10.26124/bec:2020-0002 |
MitoEAGLE muscle 1 | 2020 | Doerrier C, Gama-Perez P, Distefano G, Pesta D, Soendergaard SD, Chroeis KM, Gonzalez-Franquesa A, Goodpaster BH, Coen P, Larsen S, Gnaiger E, Garcia-Roves PM (2020) Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers. - Updated: 2020-03-08 |
MiPNet21.16 DatLab 7 Innovations | 2019-09-23 | DatLab 7: innovations. |
MiPNet18.10 O2k-Specifications | 2019-09-19 | O2k-specifications for respirometry and comprehensive OXPHOS analysis. |
MiPNet22.16 DatLab 7 Innovations: DL-Protocols | 2019-09-13 | DatLab 7.4 Innovations: DL-Protocols |
MiPNet23.12 DL-Protocols help | 2019-09-13 | DatLab Protocols: Help DatLab 7. |
MiPNet24.11 mtMP calculation | 2019-08-05 | Mitochondrial membrane potential calculation |
MiPNet20.14 AmplexRed H2O2-production | 2019-06-24 | O2k-FluoRespirometry: HRR and simultaneous determination of H2O2 production with Amplex UltraRed. |
MiPNet20.13 Safranin mt-membranepotential | 2019-06-24 | O2k-FluoRespirometry: HRR and simultaneous determination of mt-membrane potential with safranin or TMRM. |
Di Marcello 2019 MitoFit Preprint Arch EA | 2019 | Di Marcello M, Iglesias-Gonzalez J, Meszaros A, Haider M, Gnaiger E, Huete-Ortega M (2019) Partitioning between cytochrome c oxidase and alternative oxidase studied by oxygen kinetics of dark respiration in Chlamydomonas reinhardtii: a microalgae model organism. https://doi.org/10.26124/mitofit:ea19.MiPSchool.0005.v3 |
Braganza 2019 Mol Aspects Med | 2019 | Braganza A, Annarapu GK, Shiva S (2019) Blood-based bioenergetics: An emerging translational and clinical tool. Mol Aspects Med 2019:100835. |
Bettink 2019 Mitochondrion | 2019 | Bettink MAW, Harms FA, Dollee N, Specht PAC, Raat NJH, Schoonderwoerd GC, Mik EG (2019) Non-invasive versus ex vivo measurement of mitochondrial function in an endotoxemia model in rat: toward monitoring of mitochondrial therapy. Mitochondrion 50:149-57. |
Doerrier 2019 MitoFit Preprint Arch EA | 2019 | Doerrier C, Gama-Perez P, Distefano G, Pesta D, Soendergaard SD, Chroeis KM, Gonzalez-Franquesa A, Goodpaster BH, Coen P, Larsen S, Gnaiger E, Garcia-Roves PM (2019) Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers. https://doi.org/10.26124/mitofit:ea19.MiPSchool.0009 |
Naszai 2019 Sci Rep | 2019 | NĂĄszai A, Terhes E, Kaszaki J, Boros M, JuhĂĄsz L (2019) Ca(2+)N it be measured? Detection of extramitochondrial calcium movement with high-resolution FluoRespirometry. Sci Rep 9:19229. |
Germeys 2019 Genes (Basel) | 2019 | Germeys C, Vandoorne T, Bercier V, Van Den Bosch L (2019) Existing and emerging metabolomic tools for ALS research. Genes (Basel) 10:E1011. |
Chabi 2019 MitoFit Preprint Arch EA | 2019 | Chabi BĂ©atrice, Ost M, Gama-Perez P, Dahdah N, Lemieux H, Holody CD, Carpenter RG, Tepp K, Puurand M, Kaambre T, Dubouchaud H, Cortade F, Pesta D, Calabria E, Casado M, Fernandez-Ortiz M, Acuña-Castroviejo D, Villena JA, Grefte S, Keijer J, O'Brien K, Sowton A, Murray AJ, Campbell MD, Marcinek DJ, Nollet E, WĂŒst R, Dayanidhi S, Gnaiger E, Doerrier C, Garcia-Roves PM (2019) Generating reference values on mitochondrial respiration in permeabilized muscle fibers. https://doi.org/10.26124/mitofit:ea19.MitoEAGLE.0001 |
Long 2019 Methods Mol Biol | 2019 | Long Q, Huang L, Huang K, Yang Q (2019) Assessing mitochondrial bioenergetics in isolated mitochondria from mouse heart tissues using Oroboros Oxygraph-2k. Methods Mol Biol 1966:237-46. |
Gvozdjakova 2019 Bratisl Lek Listy | 2019 | Gvozdjakova A, Sumbalova Z, Kucharska J, Chladekova A, Rausova Z, Vancova O, Komlosi M, Ulicna O, Mojto V (2019) Platelet mitochondrial bioenergetic analysis in patients with nephropathies and non-communicable diseases: a new method. Bratisl Lek Listy 120:630-35. |
MiPNet23.15 O2k-pH ISE-Module | 2018-09-08 | Measurement of proton flux with the O2k-pH ISE-Module. |
MiPNet21.06 SUIT RP | 2018-06-25 | SUIT reference protocol for OXPHOS analysis by high-resolution respirometry. |
Fisher-Wellman 2018 Cell Rep | 2018 | Fisher-Wellman KH, Davidson MT, Narowski TM, Lin CT, Koves TR, Muoio DM (2018) Mitochondrial diagnostics: A multiplexed assay platform for comprehensive assessment of mitochondrial energy fluxes. Cell Rep 24:3593-606. |
Komlodi 2018 Methods Mol Biol | 2018 | Komlodi T, Sobotka O, Krumschnabel G, Bezuidenhout N, Hiller E, Doerrier C, Gnaiger E (2018) Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production. Methods Mol Biol 1782:137-55. |
Havird 2018 bioRxiv | 2018 | Havird JC, Noe GR, Link L, Torres A, Logan DC, Sloan DB, Chicco AJ (2018) Assessing mitochondrial function in angiosperms with highly divergent mitochondrial genomes. bioRxiv doi: https://doi.org/10.1101/448902. |
Van Daele 2018 Medicine (Baltimore) | 2018 | Van Daele E, Van Nieuwenhove Y, Ceelen W, Vanhove C, Braeckman BP, Hoorens A, Van Limmen J, Varin O, Van de Putte D, Willaert W, Pattyn P (2018) Assessment of graft perfusion and oxygenation for improved outcome in esophageal cancer surgery: Protocol for a single-center prospective observational study. Medicine (Baltimore) 97:e12073. |
Simard 2018 J Vis Exp | 2018 | Simard CJ, Pelletier G, Boudreau LH, Hebert-Chatelain E, Pichaud N (2018) Measurement of mitochondrial oxygen consumption in permeabilized fibers of Drosophila using minimal amounts of tissue. J Vis Exp 134. |
Smirnova 2018 Sci Rep | 2018 | Smirnova IA, Ădelroth P, Brzezinski P (2018) Extraction and liposome reconstitution of membrane proteins with their native lipids without the use of detergents. Sci Rep 8:14950. |
Puurand 2018 Int J Mol Sci | 2018 | Puurand M, Tepp K, Klepinin A, Klepinina L, Shevchuk I, Kaambre T (2018) Intracellular energy-transfer networks and high-resolution respirometry: a convenient approach for studying their function. Int J Mol Sci 19:e2933. |
Ramil 2018 Thesis | 2018 | Ramil LR, Gulbinas A, Cesna V (2018) âHIPECâ hyperthermic intraperitoneal chemotherapy. Evaluation of intraperitoneal free cancer cells. Master's Thesis. |
Doerrier 2018 Methods Mol Biol | 2018 | Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészåros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. https://doi.org/10.1007/978-1-4939-7831-1_3 |
Deschaseaux 2018 US Patent | 2018 | Deschaseaux F, Guilloton F, Muller S, Sensebe L, Casteilla L, Carriere-Pazat A (2018) Method for obtaining human brown/beige adipocytes . US Patent US20180216070A1. |
Dias 2018 Anal Biochem | 2018 | Dias C, Lourenco CF, Barbosa RM, Laranjinha J, Ledo A (2018) Analysis of respiratory capacity in brain tissue preparations: high-resolution respirometry for intact hippocampal slices. Anal Biochem 551:43-50. |
Fischer 2018 BMC Musculoskelet Disord | 2018 | Fischer MJ, Horvath G, Krismer M, Gnaiger E, Goebel G, Pesta DH (2018) Evaluation of mitochondrial function in chronic myofascial trigger points - a prospective cohort pilot study using high-resolution respirometry. BMC Musculoskelet Disord 19:388. |
Run DL-Protocol/Set O2 limit | 2017-08-10 | Online documentation of DL-Protocol function of DatLab7. |
Djafarzadeh 2017 J Vis Exp | 2017 | Djafarzadeh S, Jakob SM (2017) High-resolution respirometry to assess mitochondrial function in permeabilized and intact cells. J Vis Exp 8.doi: 10.3791/54985.. |
Vienne 2017 Anal Biochem | 2017 | Vienne JC, Cimetta C, Dubois M, Duburcq T, Favory R, Dessein AF, Fontaine M, Joncquel-Chevalier Curt M, Cuisset JM, Douillard C, Mention-Mulliez K, Dobbelaere D, Vamecq J (2017) A fast method for high resolution oxymetry study of skeletal muscle mitochondrial respiratory chain complexes. Anal Biochem 528:57-62. |
Westerlund 2017 Pediatr Res | 2017 | Westerlund E, Marelsson SE, Ehinger JK, Sjövall F, Morota S, à sander Frostner E, Oldfors A, Darin N, Lundgren J, Hansson MJ, Fellman V, Elmér E (2017) Oxygen consumption in platelets as an adjunct diagnostic method for pediatric mitochondrial disease. Pediatr Res 83:455-65. |
Djafarzadeh 2017b J Vis Exp | 2017 | Djafarzadeh S, Jakob SM (2017) Isolation of intact mitochondria from skeletal muscle by differential centrifugation for High-Resolution Respirometry measurements. J Vis Exp doi: 10.3791/55251. |
MiPNet21.14 Reference sample HRR | 2016-10-19 | Development of a reference sample for HRR. |
MiPNet21.13 Comparison mt-preparations | 2016-10-18 | O2k-Protocols: Mitochondrial preparations for HRR. |
MiPNet21.12 Comparison mt respiration media | 2016-10-18 | O2k-MultiSensor: Mitochondrial respiration media for HRR and simultaneous O2k-Fluorometry. |
MiPNet19.18C DatLab 6: Guide | 2016-08-24 | DatLab-guide through the menus. |
MiPNet19.18D O2k-Series G and DatLab 6: Calibration | 2016-08-24 | O2k-calibration by DatLab 6. |
MiPNet08.09 CellRespiration | 2016-08-11 | High-resolution respirometry and coupling-control protocol with living cells: ROUTINE, LEAK, ET-pathway, ROX. |
MiPNet19.18A O2k-Series G: Start | 2016-08-08 | Oxygraph-2k: start high-resolution respirometry. |
MiPNet19.18 O2k-Series G Core manual | 2016-08-08 | O2k-Core manual contents. |
MiPNet19.18E O2 flux analysis - DatLab 6 | 2016-08-08 | Oxygen flux analysis: DatLab real-time. |
MiPNet10.04 CellRespiration | 2016-08-08 | An experiment with high-resolution respirometry: coupling control in cell respiration. |
MiPNet21.07 MitoFit DatLab PT | 2016-07-20 | MitoFit DatLab proficiency test. |
MiPNet15.05 NO-manual | 2016-04-21 | O2k-MultiSensor system with amperometric sensors: NO, H2S, H2O2. |
Jang 2016 J Intensive Care Med | 2016 | Jang DH, Greenwood JC, Spyres MB, Eckmann D (2016) Measurement of mitochondrial respiration and motility in acute care: sepsis, trauma, and poisoning. J Intensive Care Med 32:86-94. |
Chowdhury 2015 Biosci Rep | 2016 | Chowdhury SK, Djordjevic J, Albensi B, Fernyhough P (2015) Simultaneous evaluation of substrate-dependent oxygen consumption rates and mitochondrial membrane potential by TMRM and safranin in cortical mitochondria. Biosci Rep 36:e00286. |
Stier 2016 Methods Ecol Evol | 2016 | Stier A, Romestaing C, Schull Q, Lefol E, Robin JP, Roussel D, Bize P (2016) How to measure mitochondrial function in birds using red blood cells: a case study in the king penguin and perspectives in ecology and evolution. Methods Ecol Evol doi:10.1111/2041-210X.12724. |
Ojuka 2016 Am J Physiol Endocrinol Metab | 2016 | Ojuka E, Andrew B, Bezuidenhout N, George S, Maarman G, Madlala HP, Mendham A, Osiki PO (2016) Measurement of ÎČ-oxidation capacity of biological samples by respirometry: a review of principles and substrates. Am J Physiol Endocrinol Metab 310:E715-23. https://doi.org/10.1152/ajpendo.00475.2015 |
Fisar 2016a Folia Biol (Praha) | 2016 | Fisar Z, Hroudova J (2016) Pig brain mitochondria as a biological model for study of mitochondrial respiration. Folia Biol (Praha) 62:15-25. |
Salin 2016 Physiol Rep | 2016 | Salin K, Villasevil EM, Auer SK, Anderson GJ, Selman C, Metcalfe NB, Chinopoulos C (2016) Simultaneous measurement of mitochondrial respiration and ATP production in tissue homogenates and calculation of effective P/O ratios. Physiol Rep 10.14814/phy2.13007. |
Ruas 2016 PLOS ONE | 2016 | Ruas JS, Siqueira-Santos ES, Amigo I, Rodrigues-Silva E, Kowaltowski AJ, Castilho RF (2016) Underestimation of the maximal capacity of the mitochondrial electron transport system in oligomycin-treated cells. PLOS ONE 11:e0150967. |
Lark 2016 Am J Physiol Cell Physiol | 2016 | Lark DS, Torres MJ, Lin CT, Ryan TE, Anderson EJ, Neufer PD (2016) Direct real-time quantification of mitochondrial oxidative phosphorylation efficiency in permeabilized skeletal muscle myofibers. Am J Physiol Cell Physiol 311:C239-45. |
Khattar 2016 J Neurosci Methods | 2016 | Khattar NK, Yablonska S, Baranov SV, Baranova OV, Kretz ES, Larkin TM, Carlisle DL, Richardson RM, Friedlander RM (2016) Isolation of functionally active and highly purified neuronal mitochondria from human cortex. J Neurosci Methods 263:1-6. |
Osiki 2016 FASEB J | 2016 | Osiki PO, Ojuka E, Marmaan G (2016) An investigation of the suitability of using octanoylcarnitine together with malate as a substrate combination for assessing Beta-oxidation using High Resolution Respirometry. FASEB J 30: 1015.12 . |
Dechandt 2016 Anal Biochem | 2016 | Dechandt CRP, Couto-Lima CA, Alberici LC (2016) Triglyceride depletion of brown adipose tissue enables analysis of mitochondrial respiratory function in permeabilized biopsies. Anal Biochem 515:55-60. |
Bai 2016 PLOS ONE | 2016 | Bai F, Fink BD, Yu L, Sivitz WI (2016) Voltage-dependent regulation of Complex II energized mitochondrial oxygen flux. PLOS ONE 11:e0154982. |
Reich 2016 Thesis | 2016 | Reich V (2016) Pathophysiologie und Verfahrensentwicklung: Mechanismen der Metabolisierung von Schwefelwasserstoff im mitochondrialen Atmungskreislauf von Lungenalveolarmakrophagen. Dissertation p91. |
MiPNet17.03 Shredder vs Fibers | 2015-06-18 | Mitochondrial respiration in permeabilized fibers versus homogenate from fish liver and heart. An application study with the PBI-Shredder. |
MiPNet06.06 Chemical O2 background | 2015-04-27 | Oxygraph assay of cytochrome c oxidase activity: chemical background correction. |
Canto 2015 Curr Protoc Mouse Biol | 2015 | CantĂł C, Garcia-Roves PM (2015) High-resolution respirometry for mitochondrial characterization of ex vivo mouse tissues. Curr Protoc Mouse Biol 5:135-53. |
Makrecka-Kuka 2015 Biomolecules | 2015 | Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. https://doi.org/10.3390/biom5031319 |
Weissig 2015 Methods Mol Biol II | 2015 | Weissig V, Edeas M, eds (2015) Mitochondrial medicine: Volume II, Manipulating mitochondrial function. Methods Mol Biol 1265:1-447. |
Kake-Guena 2015 J Exp Zool A Ecol Genet Physiol | 2015 | Kake-Guena SA, Touisse K, Vergilino R, Dufresne F, Blier PU, Lemieux H1 (2015) Assessment of mitochondrial functions in Daphnia pulex clones using high-resolution respirometry. J Exp Zool A Ecol Genet Physiol 323:292-300. |
Cagnone 2015 Methods Mol Biol | 2015 | Cagnone G, Vaghjiani V, Lee W, Sun C, Johnson J, Yeung KY, St John JC (2015) Analysis of the mitochondrial DNA and its replicative capacity in induced pluripotent stem cells. Methods Mol Biol 1357:231-67. |
Kluckova 2015 Methods Mol Biol | 2015 | Kluckova K, Dong L, Bajzikova M, Rohlena J, Neuzil J (2015) Evaluation of respiration of mitochondria in cancer cells exposed to mitochondria-targeted agents. Methods Mol Biol 1265:181-94. |
Harrison 2015 J Appl Physiol | 2015 | Harrison DK, Fasching M, Fontana-Ayoub M, Gnaiger E (2015) Cytochrome redox states and respiratory control in mouse and beef heart mitochondria at steady-state levels of hypoxia. https://doi.org/10.1152/japplphysiol.00146.2015 |
Abou-Hamdan 2015 Methods Enzymol | 2015 | Abou-Hamdan A, Guedouari-Bounihi H, Lenoir V, Andriamihaja M, Blachier F, Bouillaud F (2015) Oxidation of H2S in mammalian cells and mitochondria. Methods Enzymol 554:201-28. |
Krumschnabel 2015 Methods Mol Biol | 2015 | Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. |
MiPNet20.17 MiP2015 Lucni Bouda | 2015 | Pecina P et al, eds (2015) Mitochondrial physiology conference. MiP2015. Mitochondr Physiol Network 20.17:87 pp. |
Wuest 2015 J Physiol | 2015 | WĂŒst RC, Helmes M, Stienen GJ (2015) Rapid changes in NADH and flavin autofluorescence in rat cardiac trabeculae reveal large mitochondrial complex II reserve capacity. J Physiol 593:1829-40. |
MiPNet19.01 O2k-Core Manual Series F DatLab 5 | 2014-03 | O2k-Core Manual - up to O2k-Series F and DatLab 5. |
MiPNet12.11 MitoRespiration | 2014-02-20 | Flux control ratios in isolated mitochondria. OXPHOS capacity and respiratory control. |
Karabatsiakis 2014 Transl Psychiatry | 2014 | Karabatsiakis A, Boeck C, Salinas-Manrique J, Kolassa S, Calzia E, Dietrich DE, Kolassa IT (2014) Mitochondrial respiration in peripheral blood mononuclear cells correlates with depressive subsymptoms and severity of major depression. Transl Psychiatry 4:e397. |
Laner 2014 Mitochondr Physiol Network MiP2014 | 2014 | Laner V, Gnaiger E, eds (2014) Mitochondrial physiology â methods, concepts and biomedical perspectives. MiP2014. ISBN 978-3-9502399-9-7 |
Schiffer 2014 FASEB J | 2014 | Schiffer TA, Ekblom B, Lundberg JO, Weitzberg E, Larsen FJ (2014) Dynamic regulation of metabolic efficiency explains tolerance to acute hypoxia in humans. FASEB J 28:4303-11. |
Ryan 2014 J Physiol | 2014 | Ryan TE, Brophy P, Lin CT, Hickner RC, Neufer PD (2014) Assessment of in vivo skeletal muscle mitochondrial respiratory capacity in humans by near-infrared spectroscopy: a comparison with in situ measurements. J Physiol 592:3231-41. |
Krumschnabel 2014 Methods Enzymol | 2014 | Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. https://doi.org/10.1016/B978-0-12-416618-9.00009-1 |
Larsen 2014 Anal Biochem | 2014 | Larsen S, KraunsĂže R, Gram M, Gnaiger E, Helge JW, Dela F (2014) The best approach: homogenization or manual permeabilization of human skeletal muscle fibers for respirometry? Anal Biochem 446:64-8. |
Herbst 2014 J Physiol | 2014 | Herbst EA, Paglialunga S, Gerling C, Whitfield J, Mukai K, Chabowski A, Heigenhauser GJ, Spriet LL, Holloway GP (2014) Omega-3 supplementation alters mitochondrial membrane composition and respiration kinetics in human skeletal muscle. J Physiol 592:1341-52. |
Van Bergen 2014 Mitochondrion | 2014 | Van Bergen NJ, Blake RE, Crowston JG, Trounce IA (2014) Oxidative phosphorylation measurement in cell lines and tissues. Mitochondrion 15:24-33. |
Chinopoulos 2014 Methods Enzymol | 2014 | Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. doi:10.1016/B978-0-12-416618-9.00017-0 |
Chakraborty 2014 J Cardiovasc Dis | 2014 | Chakraborty M, Phillips ARJ, Macdonald J, Windsor JA, Hickey AJR (2014) Mitochondrial respiration in mononuclear cells and heart fibers in spontaneously hypertensive rats. J Cardiovasc Dis ISSN:2330-459. |
Gnaiger 2014 MitoPathways | 2014 | Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. Oroboros MiPNet Publications, Innsbruck:80 pp. â see 5th edition: Gnaiger 2020 BEC MitoPathways. |
Pecina 2014 Biochim Biophys Acta Clinical | 2014 | Pecina P, Houstkova H, Mracek T, Pecinova A, Nuskova H.Tesarova M, Hansikova H, Janota J, Zemanc J, Houstek J (2014) Noninvasive diagnostics of mitochondrial disorders in isolated lymphocytes with high resolution respirometry. Biochim Biophys Acta Clinical 2:62â71. |
Kettunen 2013 Thesis University of Applied Sciences - Turku | 2013 | Kettunen J (2013) Ruskean rasvan hapenkulutuksen mittaaminen. Thesis University of Applied Sciences - Turku pp106. |
Perry 2013 Diabetes | 2013 | Perry CG, Kane DA, Lanza IR, Neufer PD (2013) Methods for assessing mitochondrial function in diabetes. Diabetes 62:1041-53. https://doi.org/10.2337/db12-1219 |
Sjoevall 2013 Mitochondrion | 2013 | Sjövall F, Ehinger JK, Marelsson SE, Morota S, Asander Frostner E, Uchino H, Lundgren J, Arnbjörnsson E, Hansson Magnus J, Fellman V, Elmér E (2013) Mitochondrial respiration in human viable platelets - methodology and influence of gender, age and storage. Mitochondrion 13:7-14. |
Chu 2013 PLoS One | 2013 | Chu MJ, Phillips AR, Hosking AW, Macdonald JR, Bartlett AS, Hickey AJ (2013) Hepatic mitochondrial function analysis using needle liver biopsy samples. PLoS One 8:e79097. |
Pesta 2012 Methods Mol Biol | 2012 | Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58. https://doi.org/10.1007/978-1-61779-382-0_3 |
Larsen 2012 CryoLetters | 2012 | Larsen S, Wright-Paradis C, Gnaiger E, Helge JW, Boushel RC (2012) Cryopreservation of human skeletal muscle impairs mitochondrial function. Cryo Letters 33:170-6. |
Horan 2012 J Gerontol A Biol Sci Med Sci | 2012 | Horan MP, Pichaud N, Ballard JWO (2012) Review: Quantifying mitochondrial dysfunction in complex diseases of aging. J Gerontol A Biol Sci Med Sci 67:1022-35. https://doi.org/10.1093/gerona/glr263 |
Gnaiger 2012 Mitochondr Physiol Network Bioblast 2012 | 2012 | Gnaiger E, Meissner B, Laner V, eds (2012) Bioblast 2012: Mitochondrial Competence. Mitochondr Physiol Network 17.12. Oroboros MiPNet Publications, Innsbruck:96 pp. ISBN 978-3-9502399-5-9 |
MiPNet08.12 IOC22 | 2011-12-11 | NO effect on mitochondrial oxygen kinetics at low oxygen. O2k workshop Report. |
Chinopoulos 2011 Methods Mol Biol | 2011 | Chinopoulos C, Zhang SF, Thomas B, Ten V, Starkov AA (2011) Isolation and functional assessment of mitochondria from small amounts of mouse brain tissue. Methods Mol Biol 793:311-24. |
Hench 2011 PloS One | 2011 | Hench J, Bratic Hench I, Pujol C, Ipsen S, Brodesser S, Mourier A, Tolnay M, Frank S, Trifunovic A (2011) A tissue-specific approach to the analysis of metabolic changes in Caenorhabditis elegans. PloS One 6:e28417. |
Scandurra 2010 Adv Exp Med Biol | 2010 | Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. https://doi.org/10.1007/978-1-4419-1241-1_2 |
MiPNet06.05 Test Experiments on O2k-Specifications | 2010 | Gnaiger E (2010) Test experiments on specifications of the Oroboros Oxygraph. Mitochondr Physiol Network 6.5. |
Sommer 2010 Eur Respir J | 2010 | Sommer N, Pak O, Schörner S, Derfuss T, Krug A, Gnaiger E, Ghofrani HA, Schermuly RT, Huckstorf C, Seeger W, Grimminger F, Weissmann N (2010) Mitochondrial cytochrome redox states and respiration in acute pulmonary oxygen sensing. https://doi.org/10.1183/09031936.00013809 |
Komary 2010 Biochim Biophys Acta | 2010 | Komary Z, Tretter L, Adam-Vizi V (2010) Membrane potential-related effect of calcium on reactive oxygen species generation in isolated brain mitochondria. Biochim Biophys Acta 1797:922-8. |
Lanza 2009 Methods Enzymol | 2009 | Lanza IR, Nair KS (2009) Functional assessment of isolated mitochondria in vitro. Methods Enzymol 457:349-72. |
Benani 2009 J Neurosci Methods | 2009 | Benani A, Barquissau V, Carneiro L, Salin B, Colombani A-L, Leloup C, Casteilla L, Rigoulet M, Pénicaud L (2009) Method for functional study of mitochondria in rat hypothalamus. J Neurosci Methods 178:301-7. |
Rostrup M 2008 Amino Acids | 2008 | Rostrup M, Fossbakk A, Hauge A, Kleppe R, Gnaiger E, Haavik J (2008) Oxygen dependence of tyrosine hydroxylase. Amino Acids 34:455-64. |
Gnaiger 2008 POS | 2008 | Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial dysfunction in drug-induced toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52. |
Park 2007 Sensors IEEE | 2007 | Park J, Chang J, Choi M, Pak JJ, Lee DY, Pak YK (2007) Microfabricated Clark-type sensor for measuring dissolved oxygen. Sensors IEEE: 1412-1415. |
Mukhanov 2006 Thermoc Acta | 2006 | Mukhanov VS, Kemp RB (2006) Simultaneous photocalorimetric and oxygen polarographic measurements on Dunaliella maritima cells reveal a thermal discrepancy that could be due to nonphotochemical quenching. Thermochimica Acta 446:11-19. |
Labajova 2006 Anal Biochem | 2006 | Labajova A, Vojtiskova A, Krivakova P, Kofranek J, Drahota Z, Houstek J (2006) Evaluation of mitochondrial membrane potential using a computerized device with a tetraphenylphosphonium-selective electrode. Anal Biochem 353:37-42. |
Huetter 2006 Exp Gerontol | 2006 | HĂŒtter E, Unterluggauer H, Garedew A, Jansen-DĂŒrr P, Gnaiger E (2006) High-resolution respirometry - a modern tool in aging research. Exp Gerontol 41:103-9. |
Garedew 2005 Eur Shock Soc | 2005 | Garedew A, HĂŒtter E, Haffner B, Gradl P, Gradl L, Jansen-DĂŒrr P, Gnaiger E (2005) High-resolution respirometry for the study of mitochondrial function in health and disease. The Oroboros O2k. Proc 11th Congress Eur Shock Soc, Vienna, Austria (Redl H, ed) Medimond, Bologna:107-11. |
Doeller 2005 Anal Biochem | 2005 | Doeller JE, Isbell TS, Benavides G, Koenitzer J, Patel H, Patel RP, Lancaster JR Jr, Darley-Usmar VM, Kraus DW (2005) Polarographic measurement of hydrogen sulfide production and consumption by mammalian tissues. Anal Biochem 341:40-51. |
Kraft 2004 Thesis UniversitÀt Halle-Wittenberg - Germany | 2004 | Kraft A (2004) LangzeitstabilitÀt der Mitochondrienfunktion permeabilisierter Muskelfasern in der Diagnostik mitochondrialer Enzephalomyopathien. Thesis UniversitÀt Halle-Wittenberg - Germany 74pp. |
Kalbacova 2003 Cytometry | 2003 | KalbĂĄcovĂĄ M, VrbackĂœ M, Drahota Z, MelkovĂĄ Z (2003) Comparison of the effect of mitochondrial inhibitors on mitochondrial membrane potential in two different cell lines using flow cytometry and spectrofluorometry. Cytometry 52A:110-6. |
Kuznetsov 2002 Anal Biochem | 2002 | Kuznetsov AV, Strobl D, Ruttmann E, Königsrainer A, Margreiter R, Gnaiger E (2002) Evaluation of mitochondrial respiratory function in small biopsies of liver. Anal Biochem 305:186-94. |
MiPNet06.01 O2k-Overview | 2001 | Gnaiger E (2001) The Oxygraph for high-resolution respirometry. Mitochondr Physiol Network 06.01. |
Gnaiger 2001 Respir Physiol | 2001 | Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. https://doi.org/10.1016/S0034-5687(01)00307-3 |
Gnaiger 2000 Life in the Cold | 2000 | Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000) Mitochondria in the cold. In: Life in the Cold (Heldmaier G, Klingenspor M, eds) Springer, Berlin, Heidelberg:431-42. https://doi.org/10.1007/978-3-662-04162-8_45 |
MiPNet04.05 Titration-Injection | 1999-01 | From step titration to ramp injection: Uncoupling by FCCP with TIP. |
Boveris 1999 Methods Enzymol | 1999 | Boveris A, Costa LE, Cadenas E, Poderoso JJ (1999) Regulation of mitochondrial respiration by ADP, O2 and NO. Methods Enzymol 301:188-98. |
Kuznetsov 1998 BTK | 1998 | Kuznetsov AV, Lassnig B, Margreiter R, Gnaiger E (1998) Diffusion limitation of oxygen versus ADP in permeabilized muscle fibers. In BioThermoKinetics in the Post Genomic Era (Larsson C, PÄhlman I-L, Gustafsson L, eds) Chalmers Reproservice, Göteborg:273-6. |
Gnaiger 1998 BTK-HRR | 1998 | Gnaiger E, Kuznetsov AV, Lassnig B, Fuchs A, Reck M, Renner K, Stadlmann S, Rieger G, Margreiter R (1998) High-resolution respirometry. Optimum permeabilization of the cell membrane by digitonin. In BioThermoKinetics in the Post Genomic Era (Larsson C, Pahlman I-L, Gustafsson L, eds) Chalmers Reproservice, Göteborg:89-95. |
Gnaiger 1998 Biochim Biophys Acta | 1998 | Gnaiger E, Lassnig B, Kuznetsov AV, Margreiter R (1998) Mitochondrial respiration in the low oxygen environment of the cell: Effect of ADP on oxygen kinetics. Biochim Biophys Acta 1365:249-54. https://doi.org/10.1016/S0005-2728(98)00076-0 |
MiPNet02.05 DatLab2 O2Kinetics | 1997-01 | DatLab 2. Analysis of oxygen kinetics. |
Gnaiger 1995 J Bioenerg Biomembr | 1995 | Gnaiger E, Steinlechner-Maran R, Méndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. https://doi.org/10.1007/BF02111656 |
Haller 1994 Anal Biochem | 1994 | Haller T, Ortner M, Gnaiger E (1994) A respirometer for investigating oxidative cell metabolism. Toward optimization of respiratory studies. Anal Biochem 218:338-42. |
Mendez 1994 BTK-191 | 1994 | Méndez G, Gnaiger E (1994) How does oxygen pressure control oxygen flux in isolated mitochondria? A methodological approach by high-resolution respirometry and digital data analysis. In: What is Controlling Life? (Gnaiger E, Gellerich FN, Wyss M, eds) Modern Trends in BioThermo-Kinetics 3. Innsbruck Univ Press:191-4. |
MiPNet03.03 Internat-Oxygraph-Workshops | 1991- | Innsbruck AT, since 1991. International oxygraph workshops on high-resolution respirometry, IOC. |
- Gnaiger E, Forstner H, eds (1983) Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Springer, Berlin, Heidelberg, New York: 370 pp. »Contents
O2k-Publications: Instruments; methods - Abstracts
Sort in ascending/descending order by a click on one of the small symbols in squares below.
Year | Reference | |
---|---|---|
Cardoso 2023 BPS2023 San Diego | 2023 | Cardoso LHD, Timon-Gomez A, Doerrier C, Garcia-Souza LF, Gnaiger Erich (2023) Respirometric substrate-uncoupler-inhibitor titration protocols for carbohydrate and fatty acid metabolism: from heart to brain mitochondria. 67th Annual Meeting of the Biophysical Society. |
Schmitt 2023 BPS2023 San Diego | 2023 | Schmitt S, Cardoso LHD, Cecatto C, Komlodi T, Gnaiger Erich (2023) Mitochondrial bioenergetics â a quantitative analytical and diagnostic approach. 67th Annual Meeting of the Biophysical Society. |
Baglivo 2023 ESCI Prague | 2023 | Baglivo E, Timon-Gomez A, Gnaiger E (2023) The cytochrome c test for respirometric quality control of the integrity of the mitochondrial outer membrane. 57th Annual Scientific Meeting of the European Society for Clinical Investigation. |
Komlodi 2022 Abstract Bioblast | 2022 | 3.1. «10+5» KomlĂłdi Timea, Tretter L (2022) The protonmotive force â not merely membrane potential. Bioblast 2022: BEC Inaugural Conference. In: https://doi.org/10.26124/bec:2022-0001 »MitoFit Preprint« ![]() |
Cecatto 2022 EBEC | 2022 | Cecatto C, Cardoso LHD, Gnaiger E (2022) Mitochondrial calcium uptake capacity is lower than calcium retention capacity in the presence and absence of cyclosporin A. EBEC 2022. |
Gnaiger 2022 ESCI Bari | 2022 | Gnaiger E (2022) Bioenergetic cluster analysis â diagnostic evaluation of mitochondrial respiratory control in human fibroblasts. 56th Annual Scientific Meeting of the European Society for Clinical Investigation. |
Baglivo 2022a ESCI Bari | 2022 | Baglivo E, Cardoso LHD, Cecatto C, Gnaiger E (2022) Quality control of mitochondrial respiration medium in high-resolution respirometry with living and permeabilized cells. 56th Annual Scientific Meeting of the European Society for Clinical Investigation. |
Baglivo 2022b ESCI Bari | 2022 | Baglivo E, Cardoso LHD, Cecatto C, Gnaiger E (2022) Statistical analysis of instrumental reproducibility in high-resolution respirometry. 56th Annual Scientific Meeting of the European Society for Clinical Investigation. |
Gnaiger 2022 Abstract Bioblast-PB | 2022 | P03. Went N, Di Marcello M, Gnaiger Erich (2022) Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by high-resolution PhotoRespirometry. Bioblast 2022: BEC Inaugural Conference. In: https://doi.org/10.26124/bec:2022-0001 »MitoFit Preprint« |
Axelrod 2022 Abstract Bioblast | 2022 | 3.6. «5 min» Axelrod Christopher L, Heintz EC, Albaugh VL, Kirwan JP (2022) Segmental regulation of intestinal mitochondrial function. Bioblast 2022: BEC Inaugural Conference. In: https://doi.org/10.26124/bec:2022-0001 ![]() |
Cardoso 2022b EBEC | 2022 | Baglivo E, Cardoso LHD, Cecatto C, Gnaiger E (2022) Instrumental quality control and evaluation of mitochondrial respiration medium in high-resolution respirometry with living and permeabilized cells. EBEC 2022. |
Doerrier 2020 PaduaMuscleDays | 2020 | Doerrier C, Di Marcello M, Gnaiger E (2020) Commitment to reproducibility in targeting mitochondrial respiratory control: basic and advanced applications of the O2k-FluoRespirometer. PaduaMuscleDays. |
Doerrier 2019 MiP2019 | 2019 | Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers. |
Chabi 2019 MiP2019 | 2019 | Generating reference values on mitochondrial respiration in permeabilized muscle fibers. |
Dahdah 2019 MiP2019 | 2019 | Approaches to assess mitochondrial respiration in white adipose tissue. |
Garcia-Roves 2019 MiPschool Coimbra | 2019 | Generating reference values on mitochondrial respiration in permeabilized muscle fibers. |
Calabria 2019 MiPschool Coimbra | 2019 | Investigating mitochondrial function in blood cells by high-resolution respirometry: contribution to an interlaboratory study. MitoEAGLE WG4. |
Doerrier 2018 MiP2018c | 2018 | Inter-laboratory study on human permeabilized myofibers: effects of fiber preparation, respiration buffer, and blebbistatin on mitochondrial respiratory capacity and oxygen limitation. |
Cardoso 2018 MiPschool Tromso E2 | 2018 | Concomitant respiration and ATP production measurements to analyse P»/O2 ratios at physiological normoxia. |
Garcia-Roves 2018 MiP2018b | 2018 | Generating mitochondrial respirometry reference values from permeabilized mouse soleus muscle fibers. Working Group 2 report. Garcia-Roves_Presentation |
Pranger 2018 Emerging Concepts in Mitochondrial Biology | 2018 | High-resolution respirometry with a reduced chamber volume â which are the compromises in OXPHOS analysis? |
Chang 2018 Life Sciences Meeting 2018 Innsbruck AT | 2018 | pH dependence of mitochondrial respiration and H2O2 production in oral cancer cells â a pilot study. |
Krajcova 2018 MiPschool Tromso E2 | 2018 | High-resolution respirometry to assess function of mitochondria in native homogenates of human heart muscle. |
Garcia-Souza 2018 Life Sciences Meeting 2018 Innsbruck AT | 2018 | A respirometric cell viability test for peripheral-blood mononuclear cells and platelets. |
Sobotka 2018 MiP2018 | 2018 | Measurement of ROS production under hypoxia and unexpected methodological pitfalls of Amplex UltraRed assay. |
Huete-Ortega 2018 MiPschool Tromso E2 | 2018 | High-resolution respirometry: new perspectives for the study of bioenergetics in algae and plants. |
Keller 2018 MiP2018 | 2018 | The structural molecular diversity of mitochondrial cardiolipins. |
Ganetzky 2018 IOC134 | 2018 | SUIT protocol development for zebrafish embryos. |
Keppner 2018 MiP2018 | 2018 | Effects of cryopreservation of peripheral blood mononuclear cells. |
Doerrier 2018 MiP2018b | 2018 | Evaluation of anaplerotic pathways to avoid artefacts in respirometric measurement of fatty acid oxidation. |
Meszaros 2017 MiPschool Obergurgl | 2017 | (Very) High-resolution respirometry â a deep dive into oxygen dependence of mitochondrial respiration. |
Laner 2016 Abstract MitoFit Science Camp 2016 | 2016 | OXPHOS and ET-capacity in permeabilized fibers of canine superathletes. Analysis of HRR data presenting unexpected challenges. |
Sparagna 2016 Abstract IOC109 | 2016 | Sparagna GC (2016) Measuring cardiac mitochondrial function in pediatric patients before and after implantation of a left ventricular assist device. Mitochondr Physiol Network 21.01. |
Sumbalova 2016b Abstract MitoFit Science Camp 2016 | 2016 | Human blood cells: isolation and HRR. |
Markova 2016 Abstract MitoFit Science Camp 2016 | 2016 | Porcine hearts samples preparation: PBI-Shredder or saponin? |
Gnaiger 2016 Abstract SFRR Australasia 2016 | 2016 | Facts and artefacts in measurements of H2O2 production under hypoxia. |
Krumschnabel 2016b Abstract MitoFit Science Camp 2016 | 2016 | O2k-MultiSensor: Mitochondrial respiration media for HRR and simultaneous O2k-Fluorometry. |
Sumbalova 2016 Abstract Mito Xmas Meeting Innsbruck | 2016 | Methods for evaluation of mitochondrial membrane potential with multi-sensor high-resolution respirometry: potentiometry vs fluorometry. |
Bezuidenhout 2016b Abstract MitoFit Science Camp 2016 | 2016 | Analysis and comparison of mitochondrial function using cooling plate or ice during mechanical separation of mouse skeletal muscle fibers. |
Krumschnabel 2016 Abstract Mito Xmas Meeting Innsbruck | 2016 | Establishment of a reference sample for high-resolution respirometry. |
Krumschnabel 2016a Abstract MitoFit Science Camp 2016 | 2016 | O2k-Protocols: Mitochondrial preparations for HRR. |
Volani 2015 Abstract IOC106 | 2015 | Volani C, Haschka D, Demetz E, Doerrier C, Gnaiger E, Weiss G (2015) Determination of mitochondrial respiration in peripheral blood mononuclear cells. Mitochondr Physiol Network 20.10. |
Kaambre 2015 Abstract MiP2015 | 2015 | Quantitative characterization of respiratory parameters of human colorectal and breast cancer clinical material. |
Ehinger 2015 Abstract MiPschool London 2015 | 2015 | Cell-permeable mitochondrial complex II substrates â a new compound class for treatment of complex I-linked mitochondrial disease. |
Abdel-Rahman 2015 Abstract MiPschool Cape Town 2015 | 2015 | Assessment of NADPH-dependent oxygen consumption and hydrogen peroxide production by high-resolution respirometry and EPR spectroscopy. |
Shevchuk 2015 Abstract MiP2015 | 2015 | Analysis of energy fluxes in colorectal cancer saponin skinned tissues and Caco-2 cells. |
Hoppel 2015 Abstract MiPschool London 2015 | 2015 | Human blood cell types as study models of mitochondrial respiration and H2O2 production. |
Laner 2015 Abstract MiPschool Cape Town 2015 | 2015 | The Oroboros Oxygraph-2k for high-resolution respirometry and O2k-Fluorometry: a new quality in OXPHOS analysis. |
Doerrier 2015 Abstract MiP2015 | 2015 | Simultaneous measurement of mitochondrial respiration, hydrogen peroxide production, and NADH autofluorescence to assess mitochondrial function. |
Laner 2015 Abstract MiPschool Greenville 2015 | 2015 | Comparative mitochondrial physiology: OXPHOS and ET capacity in permeabilized fibers of canine superathletes. |
Maarman 2015 Abstract MiPschool Cape Town 2015 | 2015 | Characterisation of mitochondrial function in a model of pulmonary hypertension-induced right ventricular failure. |
Sumbalova 2015 Abstract MiP2015 | 2015 | High-resolution measurement of mitochondrial membrane potential and respiration â comparison of potentiometric and fluorometric methods. |
Doerrier 2015 Abstract MiPschool Greenville 2015 | 2015 | "Succinate paradox" study by high resolution respirometry combined with fluorometry and spectrofluorometry: NextGen-O2k. |
Mussulini 2015 Abstract MiPschool London | 2015 | Changes of brain substrates preference and metabolism during seizure induced by pentylenetetrazole in adult zebrafish. |
Laner 2015 Abstract MiP2015 | 2015 | Comparative mitochondrial physiology: OXPHOS and ET capacity in permeabilized fibers of canine superathletes. |
Gnaiger 2015 Abstract MiP2015 | 2015 | Mitochondrial function of cyropreserved HEK 293T cells: development of a reference sample for high-resolution respirometry. |
Pettersen 2015 Abstract MiPschool London 2015 | 2015 | Metabolic adjustments after reversible induction of mitochondrial biogenesis via AMPK/NRF1 in HeLa cells. |
Sumbalova 2014 Abstract MiP2014 | 2014 | Optimization of malate concentration for high-resolution respirometry: mitochondria from rat liver and brain. |
Ali 2014 Abstract MiP2014 | 2014 | Oxygen-consuming and ROS-producing activities in synaptosomes. |
Meszaros 2014 Abstract MiP2014 | 2014 | Methodological aspects of assessing mitochondrial function in small intestinal mucosal samples of rats and guinea pigs. |
Perry 2014 Abstract MiP2014 | 2014 | Evaluation of critical experimental parameters for assessing mitochondrial bioenergetics in permeabilized myofibers. |
Krumschnabel 2013 Abstract MiP2013 | 2013 | Krumschnabel G, Eigentler A, Fontana-Ayoub M, Draxl A, Fasching M, Gnaiger E (2013) Tissue homogenates for respiratory OXPHOS analysis in comparative mitochondrial physiology: mouse and trout â heart and liver. Mitochondr Physiol Network 18.08. |
Laner 2013 ASMRM | 2013 | Laner V, Gradl P, Eigentler A, Fontana-Ayoub M, Fasching M, Gnaiger E (2013) The Oroboros O2k for high-resolution respirometry and O2k-Fluorometry: a new quality in OXPHOS analysis. ASMRM2013. |
Cervinkova 2012 Abstract Bioblast | 2012 | Cervinkova Z, Stankova P, Cervinka M, Kucera O, Drahota Z (2012) Evaluation of mitochondrial functions â an important tool for experimental hepatology. Mitochondr Physiol Network 17.12. |
Fasching 2012 Abstract Bioblast | 2012 | Fasching M, Sumbalova Z, Gnaiger E (2012) O2k-Fluorometry - a MitoCom project. Mitochondr Physiol Network 17.12. |
Sumbalova 2011 Abstract Kagoshima | 2011 | Sumbalova Z, Harrison DK, Gradl P, Fasching M, Gnaiger E (2011) Mitochondrial membrane potential, coupling control, H2O2 production, and the upper limit of mitochondrial performance. Abstract Kagoshima. |
Fasching 2011 Abstract Berlin | 2011 | Fasching M, Harrison DK, Tretter L, Gnaiger E (2011) Combination of high-resolution respirometry and fluorometry for continuous monitoring of hydrogen peroxide production by mitochondria with resolution in the nanomolar range. Abstract Berlin. |
O2k-Manual and protocols
- » O2k-Manual
- » O2k-Protocols