O2k-Publications: O2k-Protocol

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O2k-Publications: O2k-Protocol

»O2k-Publications: Topics

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	Year	Reference	Organism	Tissue;cell
MiPNet24.10 H2O2 flux analysis	2019-08-01	Hydrogen peroxide flux analysis using Amplex UltraRed assay in MiR05-Kit with DatLab 7.4
MiPNet24.06 Oxygen flux analysis - DatLab 7.4	2019-07-26	Oxygen flux analysis with DatLab 7.4.
MiPNet24.08 Safranin Analysis Template	2019-07-26	Excel template for safranin data analysis.
MiPNet24.09 General Template for Mt-membrane Potential Analysis	2019-07-26	General template for mitochondiral membrane potential analysis
MiPNet23.12 DL-Protocols help	2019-07-01	DatLab Protocols: Help DatLab 7.
MiPNet22.16 DatLab 7 Innovations: DL-Protocols	2019-07-01	DatLab 7.4 Innovations: DL-Protocols
MiPNet18.06 Amplex-Yeast	2019-06-24	O2k-FluoRespirometry: Amplex UltraRed using freeze-dried baker’s yeast.	Saccharomyces cerevisiae Fungi
MiPNet20.13 Safranin mt-membranepotential	2019-06-24	O2k-FluoRespirometry: HRR and simultaneous determination of mt-membrane potential with safranin or TMRM.	Mouse	Nervous system
MiPNet18.05 Amplex-Mouse-heart	2019-06-24	O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate.	Mouse	Heart
MiPNet20.14 AmplexRed H2O2-production	2019-06-24	O2k-FluoRespirometry: HRR and simultaneous determination of H2O2 production with Amplex UltraRed.	Mouse	Heart
MiPNet12.23 FiberRespiration	2019-01-10	Mitochondrial respiration in permeabilized fibers: Needle biopsies from horse skeletal muscle.	Horse	Skeletal muscle
Havird 2019 Mitochondrion	2019	Havird JC, Noe GR, Link L, Torres A, Logan DC, Sloan DB, Chicco AJ (2019) Do angiosperms with highly divergent mitochondrial genomes have altered mitochondrial function? Mitochondrion 49:1-11.	Plants
Long 2019 Methods Mol Biol	2019	Long Q, Huang L, Huang K, Yang Q (2019) Assessing mitochondrial bioenergetics in isolated mitochondria from mouse heart tissues using Oroboros Oxygraph-2k. Methods Mol Biol 1966:237-46.	Mouse	Heart
Gnaiger 2019 MitoPathways	2019	Gnaiger E (2019) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Mitochondr Physiol Network 24.05. Oroboros MiPNet Publications, Innsbruck:112 pp.	Human Mouse	Heart Skeletal muscle Fibroblast
MiPNet12.01 Suppl T-issue	2018-11-18	MitoPathways at the Q-junction: mouse skeletal muscle fibers.	Mouse	Skeletal muscle
MiPNet21.16 DatLab 7 Innovations	2018-07-12	DatLab 7: innovations.
MiPNet21.06 SUIT RP	2018-06-25	SUIT reference protocol for OXPHOS analysis by high-resolution respirometry.
MiPNet21.17 BloodCellsIsolation	2018-04-09	O2k-Protocols: Isolation of blood cells for HRR.	Human	Blood cells Lymphocyte Platelet
Komlodi 2018 J Bioenerg Biomembr	2018	Komlódi T, Geibl FF, Sassani M, Ambrus A, Tretter L (2018) Membrane potential and delta pH dependency of reverse electron transport-associated hydrogen peroxide production in brain and heart mitochondria. J Bioenerg Biomembr 10.1007/s10863-018-9766-8.	Guinea pig	Heart Nervous system
Doerrier 2018 Methods Mol Biol	2018	Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70.	Human Mouse Rat Saccharomyces cerevisiae	Heart Skeletal muscle Endothelial;epithelial;mesothelial cell Blood cells HEK Platelet
Komlodi 2018 Methods Mol Biol	2018	Komlodi T, Sobotka O, Krumschnabel G, Bezuidenhout N, Hiller E, Doerrier C, Gnaiger E (2018) Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production. Methods Mol Biol 1782:137-55.	Human Mouse	Skeletal muscle HEK
Oxygen calibration - DatLab	2017-08-23	Online documentation of O2 calibration in DatLab7.
MiPNet15.09. Yeast: HRR Reference Assay	2017-02-05	A mitochondrial reference assay for high-resolution respirometry using freeze-dried Baker’s yeast.	Saccharomyces cerevisiae Fungi
Lemieux 2017 Sci Rep	2017	Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840, DOI:10.1038/s41598-017-02789-8.	Mouse	Heart
MiPNet08.09 CellRespiration	2016-08-11	High-resolution respirometry and coupling control protocol with living cells: ROUTINE, LEAK, ET-pathway, ROX.	Human	Blood cells Lymphocyte
MiPNet17.05 O2k-Fluo LED2-Module	2016-08-09	O2k-Fluo LED2-Module.
MiPNet19.18E O2 flux analysis - DatLab 6	2016-08-08	Oxygen flux analysis: DatLab real-time.
MiPNet10.04 CellRespiration	2016-08-08	An experiment with high-resolution respirometry: coupling control in cell respiration.	Mouse	Blood cells
MiPNet21.07 MitoFit DatLab PT	2016-07-20	MitoFit DatLab proficiency test.	Human
MiPNet14.05 TPP-mtMembranePotential	2016-04-01	TPP+ and mt-membrane potential.	Mouse	Nervous system
Schoepf 2016 FEBS J	2016	Schöpf B, Schäfer G, Weber A, Talasz H, Eder IE, Klocker H, Gnaiger E (2016) Oxidative phosphorylation and mitochondrial function differ between human prostate tissue and cultured cells. FEBS J 283:2181-96.	Human	Endothelial;epithelial;mesothelial cell Genital Other cell lines Fibroblast
Doerrier 2016 Mitochondrion	2016	Doerrier C, García JA, Volt H, Díaz-Casado ME, Luna-Sánchez M, Fernández-Gil B, Escames G, López LC, Acuña-Castroviejo D (2016) Permeabilized myocardial fibers as model to detect mitochondrial dysfunction during sepsis and melatonin effects without disruption of mitochondrial network. Mitochondrion 27:56-63.	Mouse	Heart
MiPNet17.15 PBI-Shredder Mouse-heart-brain-liver	2015-06-22	Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain and liver.	Mouse	Heart Nervous system Liver
MiPNet17.03 Shredder vs Fibers	2015-06-18	Mitochondrial respiration in permeabilized fibers versus homogenate from fish liver and heart. An application study with the PBI-Shredder.	Fishes	Heart Liver
MiPNet17.16 O2k-Spectrophotometry	2015-06-16	O2k-Spectrophotometry - A MitoCom Project.
Gnaiger 2015 Scand J Med Sci Sports	2015	Gnaiger E, Boushel R, Søndergaard H, Munch-Andersen T, Damsgaard R, Hagen C, Díez-Sánchez C, Ara I, Wright-Paradis C, Schrauwen P, Hesselink M, Calbet JAL, Christiansen M, Helge JW, Saltin B (2015) Mitochondrial coupling and capacity of oxidative phosphorylation in skeletal muscle of Inuit and caucasians in the arctic winter. Scand J Med Sci Sports 25 (Suppl 4):126–34.	Human	Skeletal muscle
Makrecka-Kuka 2015 Biomolecules	2015	Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38.	Human Mouse	Heart Nervous system HEK
Krumschnabel 2015 Methods Mol Biol	2015	Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61.	Mouse	Nervous system
MiPNet12.11 MitoRespiration	2014-02-20	Flux control ratios in isolated mitochondria. OXPHOS capacity and respiratory control.	Artemia Crustaceans
Krumschnabel 2014 Methods Enzymol	2014	Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81.	Mouse	Nervous system
Van Bergen 2014 Mitochondrion	2014	Van Bergen NJ, Blake RE, Crowston JG, Trounce IA (2014) Oxidative phosphorylation measurement in cell lines and tissues. Mitochondrion 15:24-33.	Human Mouse	Nervous system Blood cells Lymphocyte
Gnaiger 2014 MitoPathways	2014	Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. Oroboros MiPNet Publications, Innsbruck:80 pp.	Human Mouse	Heart Skeletal muscle Fibroblast
Pesta 2012 Methods Mol Biol	2012	Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58.	Human	Fibroblast HEK HUVEC Other cell lines Skeletal muscle
Votion 2012 PLoS One	2012	Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7:e34890.	Horse	Skeletal muscle
MiPNet15.08 TPP electrode	2011-12-11	Ion selective electrode for TPP+ and high-resolution respirometry.
MiPNet08.12 IOC22	2011-12-11	NO effect on mitochondrial oxygen kinetics at low oxygen. O2k workshop Report.	Rat	Liver
MiPNet12.17 BovineHeartMito	2011-12-11	Mitochondrial respiration with frozen bovine heart mitochondria: diagnostic tests.	Bovines	Heart
Lemieux 2011 Int J Biochem Cell Biol	2011	Lemieux H, Semsroth S, Antretter H, Höfer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43:1729–38.	Human	Heart
Boushel 2007 Diabetologia	2007	Boushel RC, Gnaiger E, Schjerling P, Skovbro M, Kraunsoee R, Dela F (2007) Patients with Type 2 diabetes have normal mitochondrial function in skeletal muscle. Diabetologia 50:790-6.	Human	Skeletal muscle
Huetter 2004 Biochem J	2004	Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28.	Human	Fibroblast
Gnaiger 2000 Life in the Cold	2000	Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000) Mitochondria in the cold. In: Life in the Cold (Heldmaier G, Klingenspor M, eds) Springer, Heidelberg, Berlin, New York:431-42.	Rat	Heart
MiPNet04.05 Titration-Injection	1999-01	From step titration to ramp injection: Uncoupling by FCCP with TIP.	Human	Endothelial;epithelial;mesothelial cell HUVEC
Steinlechner-Maran 1996 Am J Physiol Cell Physiol	1996	Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Oxygen dependence of respiration in coupled and uncoupled endothelial cells. Am J Physiol Cell Physiol 271:C2053-61.	Human	Endothelial;epithelial;mesothelial cell HUVEC

O2k-Publications: O2k-Protocol- Abstracts

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	Year	Reference	Organism	Tissue;cell
Bastos Sant'Anna Silva 2019 Life Sciences Meeting 2019 Innsbruck AT	2019	Cellular succinate transport and mitochondrial respiratory function in prostate cancer.	Human	Genital
Garcia-Roves 2019 MiPschool Coimbra	2019	Generating reference values on mitochondrial respiration in permeabilized muscle fibers.	Mouse	Skeletal muscle
Calabria 2019 MiPschool Coimbra	2019	Investigating mitochondrial function in blood cells by high resolution respirometry: contribution to an interlaboratory study. Mitoeagle WG4.		Blood cells
Sobotka 2018 MiP2018	2018	Measurement of ROS production under hypoxia and unexpected methodological pitfalls of Amplex UltraRed assay.	Mouse Saccharomyces cerevisiae	Heart Nervous system
Doerrier 2018 ESCI2018	2018	High-resolution respirometry, two techniques combined in one to explore mitochondrial function in health and disease	Human Mouse	Heart Skeletal muscle Nervous system Blood cells HEK
Bastos Sant'Anna Silva 2018 TRANSMIT Bertinoro di Romagna IT	2018	Cellular succinate transport and mitochondrial respiratory function in prostate cancer.	Human	HEK Genital
Bastos Sant'Anna Silva 2018 MiPschool Tromso C4	2018	Role of succinate in prostate cancer cells: uptake and mitochondrial respiratory function.	Human	Genital
Garipi 2018 MiPschool Tromso E2	2018	Phenotyping mitochondrial metabolism in Barrett’s metaplasia-dysplasia-adenocarcinoma sequence: respiratory capacity, extracelular proton flux and ROS production	Human	Endothelial;epithelial;mesothelial cell Other cell lines
Chang 2018 Life Sciences Meeting 2018 Innsbruck AT	2018	pH dependence of mitochondrial respiration and H2O2 production in oral cancer cells – a pilot study.	Human	HEK Kidney
Sobotka 2017 MiPschool Obergurgl	2017	Reference protocols for ROS production measurements.
Doerrier 2016 Abstract MitoFit Science Camp 2016	2016	Development of a SUIT reference protocol for OXPHOS analysis by high-resolution respirometry.	Human Mouse	Heart Blood cells HEK Platelet
Volani 2016 Abstract Mito Xmas Meeting Innsbruck	2016	Effects of systemic iron perturbations on mitochondrial activity and on cellular metabolism in vivo.	Mouse	Liver
Sumbalova 2015 Abstract MiP2015	2015	High-resolution measurement of mitochondrial membrane potential and respiration – comparison of potentiometric and fluorometric methods.	Mouse	Nervous system
Laner 2015 Abstract MiPschool Greenville 2015	2015	Comparative mitochondrial physiology: OXPHOS and ET capacity in permeabilized fibers of canine superathletes.	Dog	Skeletal muscle
Sumbalova 2014 Abstract MiP2014	2014	Optimization of malate concentration for high-resolution respirometry: mitochondria from rat liver and brain.	Rat	Nervous system Liver