O2k-Publications: O2k-Protocol

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O2k-Publications: O2k-Protocol

O2k-Publications in the MiPMap

»O2k-Publications: Topics

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 YearReferenceOrganismTissue;cell
Baglivo 2022 BECBEC 2022.8Baglivo E, Cardoso LHD, Cecatto C, Gnaiger E (2022) Statistical analysis of instrumental reproducibility as internal quality control in high-resolution respirometry. Bioenerg Commun 2022.8. https://doi.org/10.26124/bec:2022-0008HEK
Komlodi 2021 BEC AmR-O2BEC 2021.4Komlódi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. https://doi.org/10.26124/bec:2021-0004Saccharomyces cerevisiaeOther cell lines
Komlodi 2021 BEC QBEC 2021.3Komlódi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. https://doi.org/10.26124/bec:2021-0003MouseHeart
Nervous system
Cardoso 2021 BEC MgGBEC 2021.1Cardoso LHD, Doerrier C, Gnaiger E (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. https://doi.org/10.26124/bec:2021-0001MouseHeart
Gnaiger 2020 BEC MitoPathwaysBEC 2020.2Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. https://doi.org/10.26124/bec:2020-0002Human
Mouse
Heart
Skeletal muscle
Fibroblast
Baglivo 2022 MitoFit-QC2022-05-09Baglivo E, Cardoso LHD, Cecatto C, Gnaiger E (2022) Statistical analysis of instrumental reproducibility as internal quality control in high-resolution respirometry. https://doi.org/10.26124/mitofit:2022-0018.v2 2022-08-04 Published in »Bioenergetics Communications 2022.8«HEK
MiPNet24.10 H2O2 flux analysis2021-10-22
O2k-Protocols
Hydrogen peroxide flux analysis using Amplex UltraRed assay in MiR05-Kit with DatLab 7.4
MiPNet26.10 MgG data analysis2021-09-16
O2k-Protocols
Measurement of mitochondrial ATP production using Magnesium Green: DL-Protocols and data analysis with DatLab 7.4
MiPNet24.09 Data analysis of mt-membrane potential2021-09-16
O2k-Protocols
Data analysis of mt-membrane potential.
MiPNet17.05 O2k-Fluo LED2-Module2021-06-22
O2k-Manual
O2k-Fluo LED2-Module.
MiPNet21.17 BloodCellsIsolation2020-10-22
O2k-Protocols
O2k-Protocols: Isolation of blood cells for HRR.
HumanBlood cells
Platelet
MiPNet24.08 Safranin Analysis Template2020-05-13
O2k-Protocols
Excel template for safranin data analysis.
MiPNet17.04 CitrateSynthase2020-04-18
O2k-Protocols
Laboratory protocol: Citrate synthase a mitochondrial marker enzyme.
MiPNet24.06 Oxygen flux analysis - DatLab 7.42020-04-16
O2k-Protocols
Oxygen flux analysis with DatLab 7.4.
MiPNet25.14 TPP Analysis Template2020-##-##
O2k-Protocols
Excel template for TPP data analysis.
MiPNet21.16 DatLab 7 Innovations2019-09-23
About Oroboros
DatLab 7: innovations.
MiPNet23.12 DL-Protocols help2019-09-13
O2k-Protocols
DatLab Protocols: Help DatLab 7.
MiPNet22.16 DatLab 7 Innovations: DL-Protocols2019-09-13
O2k-Protocols
DatLab 7.4 Innovations: DL-Protocols
MiPNet24.11 mtMP calculation2019-08-05
O2k-Protocols
Mitochondrial membrane potential calculation
MiPNet18.05 Amplex-Mouse-heart2019-06-24
O2k-Protocols
O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate.
MouseHeart
MiPNet20.14 AmplexRed H2O2-production2019-06-24
O2k-Protocols
O2k-FluoRespirometry: HRR and simultaneous determination of H2O2 production with Amplex UltraRed.
MouseHeart
MiPNet20.13 Safranin mt-membranepotential2019-06-24
O2k-Protocols
O2k-FluoRespirometry: HRR and simultaneous determination of mt-membrane potential with safranin or TMRM.
MouseNervous system
Long 2019 Methods Mol Biol2019Long Q, Huang L, Huang K, Yang Q (2019) Assessing mitochondrial bioenergetics in isolated mitochondria from mouse heart tissues using Oroboros Oxygraph-2k. Methods Mol Biol 1966:237-46.MouseHeart
MiPNet12.01 Suppl T-issue2018-11-18
O2k-Protocols contents
MitoPathways at the Q-junction: mouse skeletal muscle fibers.
MouseSkeletal muscle
Doerrier 2018 Methods Mol Biol2018Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. https://doi.org/10.1007/978-1-4939-7831-1_3Human
Mouse
Rat
Saccharomyces cerevisiae
Heart
Skeletal muscle
Endothelial;epithelial;mesothelial cell
Blood cells
HEK
Platelet
Komlodi 2018 Methods Mol Biol2018Komlodi T, Sobotka O, Krumschnabel G, Bezuidenhout N, Hiller E, Doerrier C, Gnaiger E (2018) Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production. Methods Mol Biol 1782:137-55.Human
Mouse
Skeletal muscle
HEK
Lemieux 2017 Sci Rep2017Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. doi:10.1038/s41598-017-02789-8MouseHeart
MiPNet08.09 CellRespiration2016-08-11
O2k-Procedures contents
High-resolution respirometry and coupling-control protocol with living cells: ROUTINE, LEAK, ET-pathway, ROX.
HumanBlood cells
Lymphocyte
MiPNet10.04 CellRespiration2016-08-08
O2k-Protocols
An experiment with high-resolution respirometry: coupling control in cell respiration.
MouseBlood cells
MiPNet19.18E O2 flux analysis - DatLab 62016-08-08
O2k-Manual
Oxygen flux analysis: DatLab real-time.
MiPNet21.07 MitoFit DatLab PT2016-07-20
O2k-Protocols
MitoFit DatLab proficiency test.
Human
MiPNet17.03 Shredder vs Fibers2015-06-18
O2k-Protocols
Mitochondrial respiration in permeabilized fibers versus homogenate from fish liver and heart. An application study with the PBI-Shredder.
FishesHeart
Liver
Gnaiger 2015 Scand J Med Sci Sports2015Gnaiger E, Boushel R, Søndergaard H, Munch-Andersen T, Damsgaard R, Hagen C, Díez-Sánchez C, Ara I, Wright-Paradis C, Schrauwen P, Hesselink M, Calbet JAL, Christiansen M, Helge JW, Saltin B (2015) Mitochondrial coupling and capacity of oxidative phosphorylation in skeletal muscle of Inuit and caucasians in the arctic winter. https://doi.org/10.1111/sms.12612HumanSkeletal muscle
Krumschnabel 2015 Methods Mol Biol2015Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61.MouseNervous system
Makrecka-Kuka 2015 Biomolecules2015Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. https://doi.org/10.3390/biom5031319Human
Mouse
Heart
Nervous system
HEK
MiPNet12.11 MitoRespiration2014-02-20
O2k-Protocols
Flux control ratios in isolated mitochondria. OXPHOS capacity and respiratory control.
Artemia
Crustaceans
Van Bergen 2014 Mitochondrion2014Van Bergen NJ, Blake RE, Crowston JG, Trounce IA (2014) Oxidative phosphorylation measurement in cell lines and tissues. Mitochondrion 15:24-33.Human
Mouse
Nervous system
Blood cells
Krumschnabel 2014 Methods Enzymol2014Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. https://doi.org/10.1016/B978-0-12-416618-9.00009-1MouseNervous system
Pesta 2012 Methods Mol Biol2012Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58. https://doi.org/10.1007/978-1-61779-382-0_3HumanSkeletal muscle
Other cell lines
HEK
Fibroblast
HUVEC
MiPNet08.12 IOC222011-12-11
O2k-Protocols
NO effect on mitochondrial oxygen kinetics at low oxygen. O2k workshop Report.
RatLiver
Lemieux 2011 Int J Biochem Cell Biol2011Lemieux H, Semsroth S, Antretter H, Höfer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43:1729–38.HumanHeart
Gnaiger 2008 POS2008Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial dysfunction in drug-induced toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52.MouseBlood cells
Gnaiger 2007 MitoPathways2007
O2k-Protocols
Gnaiger E ed (2007) Mitochondrial Pathways and Respiratory Control. Oroboros MiPNet Publications, Innsbruck:96 pp. Electronic 1st ed ISBN 978-3-9502399-0-4. — see 5th edition: Gnaiger 2020 BEC MitoPathways.
Huetter 2004 Biochem J2004Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. https://doi.org/10.1042/BJ20040095HumanFibroblast
Gnaiger 2000 Life in the Cold2000Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000) Mitochondria in the cold. In: Life in the Cold (Heldmaier G, Klingenspor M, eds) Springer, Berlin, Heidelberg:431-42. https://doi.org/10.1007/978-3-662-04162-8_45RatHeart
MiPNet04.05 Titration-Injection1999-01
O2k-Protocols contents
From step titration to ramp injection: Uncoupling by FCCP with TIP.
HumanEndothelial;epithelial;mesothelial cell
HUVEC
Steinlechner-Maran 1996 Am J Physiol Cell Physiol1996Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Oxygen dependence of respiration in coupled and uncoupled endothelial cells. Am J Physiol Cell Physiol 271:C2053-61.HumanEndothelial;epithelial;mesothelial cell
HUVEC
O2k-Publications in the MiPMap

O2k-Publications: O2k-Protocol- Abstracts

Sort in ascending/descending order by a click on one of the small symbols in squares below.
 YearReferenceOrganismTissue;cell
Bastos Sant'Anna Silva 2019 Life Sciences Meeting 2019 Innsbruck AT2019Cellular succinate transport and mitochondrial respiratory function in prostate cancer.HumanGenital
Garcia-Roves 2019 MiPschool Coimbra2019
Pablo Garcia-Roves
Generating reference values on mitochondrial respiration in permeabilized muscle fibers.
MouseSkeletal muscle
Calabria 2019 MiPschool Coimbra2019
Elisa Calabria
Investigating mitochondrial function in blood cells by high-resolution respirometry: contribution to an interlaboratory study. MitoEAGLE WG4.
Blood cells
Bastos Sant'Anna Silva 2018 TRANSMIT Bertinoro di Romagna IT2018Cellular succinate transport and mitochondrial respiratory function in prostate cancer.HumanHEK
Genital
Bastos Sant'Anna Silva 2018 MiPschool Tromso C42018
Ana Bastos Sant'Anna
Role of succinate in prostate cancer cells: uptake and mitochondrial respiratory function.
HumanGenital
Garipi 2018 MiPschool Tromso E22018Phenotyping mitochondrial metabolism in Barrett’s metaplasia-dysplasia-adenocarcinoma sequence: respiratory capacity, extracelular proton flux and ROS productionHumanEndothelial;epithelial;mesothelial cell
Other cell lines
Chang 2018 Life Sciences Meeting 2018 Innsbruck AT2018pH dependence of mitochondrial respiration and H2O2 production in oral cancer cells – a pilot study.HumanHEK
Kidney
Sobotka 2018 MiP20182018
Ondrej Sobotka
Measurement of ROS production under hypoxia and unexpected methodological pitfalls of Amplex UltraRed assay.
Mouse
Saccharomyces cerevisiae
Heart
Nervous system
Doerrier 2018 ESCI20182018
Carolina Anneliese Doerrier
High-resolution respirometry, two techniques combined in one to explore mitochondrial function in health and disease
Human
Mouse
Heart
Skeletal muscle
Nervous system
Blood cells
HEK
Sobotka 2017 MiPschool Obergurgl2017
Ondrej Sobotka
Reference protocols for ROS production measurements.
Doerrier 2016 Abstract MitoFit Science Camp 20162016Development of a SUIT reference protocol for OXPHOS analysis by high-resolution respirometry.Human
Mouse
Heart
Blood cells
HEK
Platelet
Volani 2016 Abstract Mito Xmas Meeting Innsbruck2016Effects of systemic iron perturbations on mitochondrial activity and on cellular metabolism in vivo.MouseLiver
Sumbalova 2015 Abstract MiP20152015High-resolution measurement of mitochondrial membrane potential and respiration – comparison of potentiometric and fluorometric methods.MouseNervous system
Laner 2015 Abstract MiPschool Greenville 20152015Comparative mitochondrial physiology: OXPHOS and ET capacity in permeabilized fibers of canine superathletes.DogSkeletal muscle
Sumbalova 2014 Abstract MiP20142014Optimization of malate concentration for high-resolution respirometry: mitochondria from rat liver and brain.RatNervous system
Liver