Description
OctPM: Octanoylcarnitine & Pyruvate & Malate.
MitoPathway control state: FN
SUIT protocol: SUIT-002, SUIT-005
This substrate combination supports N-linked flux which is typically higher than FAO capacity (F/FN<0 in the OXPHOS state). In SUIT-RP1, PMOct is induced after PM(E), to evaluate any additive effect of adding Oct. In SUIT-RP2, FAO OXPHOS capacity is measured first, testing for the effect of increasing malate concentration (compare malate-anaplerotic pathway control state, M alone), and pyruvate is added to compare FAO as the background state with FN as the reference state.
Abbreviation: OctPM
Reference: MiPNet21.06 SUIT RP
OctPML
FN pathway in the LEAK state can be evaluated in the following SUIT protocols:
OctPMP
FN pathway in the OXPHOS state can be evaluated in the following SUIT protocols:
-
- DL-Protocol for isolated mitochondria and tissue homogenate (mt): SUIT-002 O2 mt D005
- DL-Protocol for permeabilized fibers (pfi): SUIT-002 O2 pfi D006
- DL-Protocol for permeabilized cells (pce): SUIT-002 O2 ce-pce D007
- DL-Protocol for permeabilized fibers (pfi):SUIT-005 O2 pfi D011
-
OctPME
FN pathway in the ET state can be evaluated in the following SUIT protocols:
MitoPedia concepts:
SUIT state