Open chamber

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OROBOROS support
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Open chamber


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O2k-Open Support


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



Description

The term "open O2k-Chamber" refers to a situation in which the liquid phase is allowed to equilibrate with a gas phase, but the stopper is partially inserted using the Stopper-Spacer.

Abbreviation: O

Reference: System

Application in HRR

The liquid phase is defined as the experimental system, which is then an open system with exchange of oxygen with the gas phase, finally resulting in a stable oxygen signal with a slope over time of zero. Insert the stopper fully, siphon off any liquid from the receptacle of the stopper, and remove the stopper to a position defined by the Stopper-Spacer to include an optimum volume of the gas phase above the stirred liquid phase.
» Compare: Closed chamber



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Quality management

Oroboros quality management from the O2k to NextGen-O2k
Gnaiger Erich 2020-09-07