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O2k-Products

- a guided tour to the O2k technology
O2k-Catalogue


» Oroboros Catalogue: concept
» Complete Oroboros Product List: including former series, all spares.
» Order information: Purchase
Product Including
O2k-FluoRespirometer the modular system for high-resolution respirometry and fluorometry
O2k-Modules TIP2k , O2k-TPP+, O2k-pH, O2k-NO
O2k-Accessories and Auxiliary Tools Titration-Tools / PBI-Shredder O2k-Set / Microbalance / O2k-Dissection Tools /

Motic Microscope

O2k-Chemical-Kit MiR05-Kit, MitoKit-CII


Oroboros O2k-Catalogue - all products in current series

Oroboros
The new MitoKit-CII

The cell permeable compounds MitoKit-CII, including succinate-nv and malonate-nv, are the first generation of NeuroVive Pharmaceutical AB's prodrugs and have been developed to stimulate or inhibit Complex II of the respiratory electron transfer system in living cells. The MitoKit-CII will expand the toolbox for evaluating the role of mitochondria in disease.


» For more information: wiki.oroboros.at/index.php/MitoKit-CII
» For offers refer to: instruments@oroboros.at
MitoKit-CII
TitleProduct descriptionProduct IDImage
O2k-FluoRespirometerThe Oroboros O2k-FluoRespirometer (O2k-Series H) - the experimental system complete for high-resolution respirometry (HRR), including fluorometry and the TIP2k, allowing simultaneous monitoring of oxygen consumption together with either ROS production (AmR), mt-membrane potential (TMRM, Safranin and Rhodamine 123), Ca2+ (CaG) or ATP production (MgG).

The O2k-FluoRespirometer supports all add-on O2k-Modules: O2k-TPP+ ISE-Module, O2k-pH ISE-Module, O2k-NO Amp-Module, enabling measurement of mt-membrane potential with ion sensitive electrodes (ISE for TPP+ or TPMP+ ) or pH.


Parameters:

  • oxygen consumption
  • ROS production
  • ATP production
  • Ca2+
  • pH
  • mitochondrial membrane potential


Features of O2k-Series H:

  • Fluo-Control Unit integrated into O2k-Main Unit.
  • Smart Fluo-Sensors with pre-calibrated light intensities for direct input into DatLab 7
  • Added: spare OroboPOS
  • DL-Protocols as real-time guides for titrations in DatLab 7, context-sensitive help. Further details » MitoPedia: DatLab.
  • MiR05-Kit


The O2k is a sole source apparatus with no other instruments meeting its specifications.
10002-02
O2k-FluoRespirometer
Power O2k-FluoRespirometerPower O2k-FluoRespirometer - optional configuration as additional system for increasing output combined with the O2k-FluoRespirometer (O2k-Series H). The Power O2k-FluoRespirometer includes the TIP2k, and supports all add-on O2k-Modules of the Oroboros O2k. It can be added to an existing Oroboros O2k of any O2k-Series. This application does not require an additional ISS-Integrated Suction System and O2k-Titration Set. Furthermore, the following OroboPOS Service Tools can be used from the available O2k and are not included: OroboPOS-Mounting Tool.10003-02
Power O2k-FluoRespirometer
Startup O2k-RespirometerStartup O2k-Respirometer - the experimental system complete for basic high-resolution respirometry (HRR). The O2k-Respirometer includes the O2k-Main Unit with stainless steel housing, O2k-Assembly Kit, two OroboPOS (polarographic oxygen sensors) and OroboPOS-Service Kit, DatLab software, the ISS-Integrated Suction System and the O2k-Titration Set. The O2k-Respirometer supports all add-on O2k-Modules of the Oroboros O2k, and is a component of the O2k-FluoRespirometer.

On-line display of oxygen flux (rate of respiration) is provided in addition to the conventional 'oxygraphic' plot of oxygen concentration over time. Highest signal stability minimizes the required amounts of biological sample, and provides the basis for resolution in the extreme low-oxygen range. Peltier temperature control provides a thermal stability at ±0.002 °C in the range of 4 °C to 47 °C at typical constant room temperature. Electronically controlled PVDF or PEEK stirrers are integrated in the two-chamber design of the O2k, and a barometric pressure transducer enables automatic oxygen calibrations implemented in the DatLab software.

The O2k is a sole source apparatus with no other instruments meeting its specifications.
10022-02
Startup O2k-Respirometer notitle.png
Power O2k-Respirometer
Power-O2k

The Power O2k-Respirometer is an economical option for using additional O2k-Units to increase output in high-resolution respirometry. This application does not require an additional ISS-Integrated Suction System, an O2k-Titration Set and the O2k-Manual. Furthermore, the following OroboPOS Service Tools can be used from the available O2k and are not included: OroboPOS-Mounting Tool.

The Power O2k-Respirometer supports all add-on O2k-Modules of the Oroboros O2k.
10023-02
Power O2k notext.png
TIP2k-ModuleTIP2k-Module - Titration-Injection microPump (TIP2k) for two-channel operation with the O2k-FluoRespirometer with automatic control by DatLab of programmable titration regimes and feedback control (oxystat, pH-stat). The TIP2k-Module includes TIP2k-Accessories: 4 Microinjection syringes, 1 Pkg. TIP2k-Needle Spacers with mounting tool, 1 TIP2k-Needle Safety Support and 1 Pkg. TIP2k Filter Papers, and the newest DatLab version.11100-03
TIP2k with 200 mm3 microsyringe.JPG
O2k-TPP+ ISE-ModuleO2k-TPP+ ISE-Module: 2 potentiometric ion-selective electrodes (ISE), ISE-Service Kit and 2 PEEK-Stoppers, supported by the Oroboros O2k (O2k-Series D and higher). It also includes the newest DatLab Version. The ISE module is designed with replaceable membranes making it possible to measure different ions such as TPP+ or Ca2+ with the same electrode housing. The main application of the O2k-TPP+ ISE-Module is measurement of mitochondrial membrane potential with tetraphenylphosphonium (TPP+) or TPMP+.12300-03
TPP new.jpg
O2k-pH ISE-ModuleO2k-pH ISE-Module: two pH electrodes and reference electrodes and accessories, two black PEEK stoppers, supported by the Oroboros O2k. It also includes the newest DatLab version. Quality control: Stability of Oroboros pH electrodes is tested for drift <15 µpH·s-1·mL-1.12400-02
PH new.jpg
O2k-NO Amp-ModuleO2k-NO Amp-Module: 2 black PEEK stoppers for amperometric O2k-MultiSensor application (NO, H2S, H2O2); 1 additional hole (2.3 mm); central titration port; conical bottom; with Volume-Calibration Rings (A and B); 2 O-rings mounted on each stopper, with 16 spare O-rings (O-ring\Viton\12.5x1 mm; two boxes). The NO sensor is not included.12500-02
O2k-NO Amp-Module.jpg
PBI-Shredder O2k-SetPBI-Shredder O2k-Set: Auxiliary O2k-Tool for tissue homogenate preparation; the Shredder-Kit Box contains the heavy duty high torque SG3 driver with convertible handle, SG3 base with 3 position force setting lever (FSL), battery charger, two lithium ion batteries, Shredder-Tube Cap Tool and Shredder-Tube Ram Tool. The PBI-Shredder O2k-Set includes the Shredder-Kit Box with 100 Shredder-Tubes FT500-PS, 100 Shredder-Tubes FT500-PMS, a pair of sharp forceps for tissue dissection, a pair of scissors and a manual. Oroboros Instruments: world-wide distributor.13200-04
PBI-Shredder HRR-Set.JPG
Microbalance-SetMicrobalance-Set: Microbalance 120 g (41 g to 120 g; 0.01 mg / 0.1 mg display; 100 to 240 V) with Microbalance-Transport Case; particularly for wet weight determination of permeabilized fibres.13300-01
Microbalance Set.jpg
O2k-Dissection SetO2k-Dissection Set: for tissue preparation, set of 4 pairs of stainless steel, antimagnetic forceps and a pair of scissors.13400-01
Forcep for tissue preparation angular tip.jpg
DatLabDatLab is the O2k-Software for Data Acquisition & Analysis, specifically developed for high-resolution respirometry with the O2k-FluoRespirometer.

The newest DatLab version is DatLab 7.4, included in the O2k-FluoRespirometer. Updates of DatLab are free of charge during the warranty period of the O2k.

The minimum computer requirements are Intel-Core-2 or equivalent CPU, 2GB RAM and Windows XP. However, we recommend Intel i5 or equivalent CPU, 4GB RAM, Windows 10 and SSD. For the proper display of DatLab on your computer, please make sure the “Language settings” are set to English.
20700
DatLab
ISS-Integrated Suction SystemISS-Integrated Suction System: Suction pump 230 or 120 V with stainless steel housing, 2 liter waste bottle, filter and tubing; for siphoning off excess medium from the O2k-Stopper and for emptying the O2k-Chambers. The ISS is included as a standard component of the O2k-FluoRespirometer. Media containing living cells or microorganisms, various poisons (inhibitors, uncouplers) and mixtures of proteins and substrates are safely disposed off in the 2-litre waste bottle.

The suction pump (230 V or 120 V) is enclosed in a stainless steel housing, which holds a readily accessible mains switch, two removable receptacles for the tip of the tubing, an easily removable and safe connection to the gas filter which further connects to the fully stabilized waste bottle. The waste bottle with filter can be simply removed for emptying, cleaning and drying.

For specific country configurations, see Product ID 28101, 28102, 28103.
20810
ISS.jpg
O2k-Titration SetThe O2k-Titration Set consists of Hamilton microsyringes (6 x 10 mm3 and 3 spare plungers, 6 x 25 mm3, 1 x 50 mm3, 1 x 100 mm3, 1 x 500 mm3; fixed needles with rounded tips), provided in the Syringe Storage Box with Syringe Labels, a set of two Syringe Racks with Syringe Collars, and a set of two Tube Racks. The O2k-Titration Set is included in the O2k-FluoRespirometer and Startup O2k-Respirometer.20820-03
O2k-Titration Set.JPG
O2k-Catalogue: O2k-Assembly Kit21### O2k-Assembly Kit: The O2k-Assembly Kit is a component of the O2k-FluoRespirometer, consisting of 2 PVDF Stirrer-Bars, 2 PVDF or PEEK O2k-Stoppers, OroboPOS-Connectors (attached to the O2k-Main Unit) and cables (power supply, USB-connection). Several components of the O2k-Catalogue: O2k-Assembly Kit are included in the O2k-Service Box either for shipment or for storage.21###21###
OroboPOS-ConnectorOroboPOS-Connector (blue POM), with male connection to OroboPOS sensor head (POS) and with cable and male plug fitting into O2k-Main Unit. Two units of this item are standard components of the O2k-Assembly Kit (O2k-FluoRespirometer), mounted on the OroboPOS-Holders at the O2k-Main Unit.22200-01
POS connector and cable connection.jpg
O2k-ChamberO2k-Chamber: 16 mm inner diameter, Duran glass polished, with standard operation volume (V) of 2 cm3 (2 mL). Two units of this item are standard components of the O2k-FluoRespirometer. Separate glass chambers are available as spares. The optical properties of Duran allow application of fluorometric sensors (Duran optical properties).23100-01
Glass chamber.jpg
Stirrer-Bar\white PVDF\15x6 mmStirrer-Bar\white PVDF\15x6 mm, operated in the O2k-Chamber at constant stirring speed (standard is 750 rpm, or 12.5 Hz), to provide optimum mixing of the sample in the aqueous medium and ensure a stable signal of the polarographic oxygen sensor (OroboPOS) placed in a position of maximum current of the medium.


Two units of this item are standard components of the O2k-Assembly Kit (O2k-FluoRespirometer).

O2k-FluoRespirometer: 2 in O2k-Assembly Kit.
23210-01
Stirrer bar PVDF.jpg
Stirrer-Bar\black PEEK\15x6 mmStirrer-Bar\black PEEK\15x6 mm, for specific applications with light guides to minimize optical interference.23220-01
Stirrer-Bar black PEEK15x6 mm.JPG
Stopper\black PEEK\conical Shaft\central PortStopper\black PEEK\conical Shaft\central Port: with conical shaft and one central capillary (with PTFE, graphite, carbon fiber), Volume-Calibration Ring (A or B) for volume adjustment 1.5 to 3.2 ml; 2 mounted O-rings, with 8 spare O-rings (O-ring\Viton\12.5x1 mm). The black PEEK stoppers are required for optical O2k-MultiSensor Modules (O2k-Fluo_LED2-Module). Two units of this item are standard components of the O2k-Assembly Kit.24000-01
Stopper black PEEK conical Shaft central Port.JPG
O-ring\Viton\12.5x1 mmO-ring\Viton\12.5x1 mm, for PVDF or PEEK O2k-Stoppers, box of 8 as spares. Two spare boxes of this product are standard components of the O2k-Assembly Kit (O2k-FluoRespirometer) as well as the O2k-TPP+ ISE-Module, O2k-pH ISE-Module and the O2k-NO Amp-Module.24310-02
O-ringViton12.5x1 mm.jpg
Volume-Calibration RingVolume-Calibration Ring (A or B) PVDF for O2k-Stoppers, white; attached to the stoppers.2432A-01 or 2432B-01
Volume calibration ring A+B PVDF for Stopper.jpg
Cover-Slip\blackA Cover-Slip should be placed on top of the O2k-Stopper to minimize contamination and evaporation of liquid extruding from the capillary of the stopper. The Cover-Slips do not exert any direct effect on oxygen backdiffusion into the O2k-Chamber. Use the the Cover-Slip\black to avoid light penetration and disturbance of fluorescence signals and generally for optical measurements in the O2k. Two units of this item are standard components of the O2k-Assembly Kit.24411-01
Cover-Slip black.JPG
Stopper-SpacerStopper-Spacer (or gas spacer) to set the O2k-Stopper into a standard position for a fixed gas phase above the aqueous phase in the O2k-Chamber, during air calibration or for injection of nitrogen, argon or oxygen into the gas phase; Stopper-Spacer thickness of 4mm (a deviation of +/- 10% possible). One Stopper-Spacer is included in the O2k-Assembly Kit (O2k-FluoRespirometer), two in the O2k-TPP+ ISE-Module as standard components.24420-01
Gas spacer for Stopper.jpg
Syringe\60 ml\Gas-InjectionSyringe\60 mL\Gas-Injection, 60 mL, with spacer and stainless steel needle, flat tip, for gas injection into the O2k-Chamber. This item is a standard component of the O2k-Assembly Kit.24430-01
Gas injection syringe.jpg
O2k-Catalogue: OroboPOS components26### O2k-Catalogue: OroboPOS components26###26###
OroboPOSThe OroboPOS is a polarographic oxygen sensor (POS), with an amperometric mode of operation. The OroboPOS meets the highest quality criteria in terms of linearity, stability and sensitivity of the signal. The Clark type polarographic oxygen sensor (POS) remains the gold standard for measuring dissolved oxygen in biomedical, environmental and industrial applications over a wide dynamic oxygen range. It consists of a gold cathode, a silver/silverchloride anode and a KCl electrolyte reservoir separated from the sample by a 25 µm membrane (FEP). The main body of the OroboPOS is made of PEEK. With application of a polarization voltage (0.8 V), a current is obtained as an amperometric signal, which is converted to a voltage.26000-01
OroboPOS.JPG
OroboPOS-Seal TipOroboPOS-Seal Tip, black butyl rubber gasket with 3 mm pore. 4/Pck, for sealing the OroboPOS (sensor head) against the O2k-Chamber. Push the wetted OroboPOS-Seal Tip over the POS-sensor head, with the pore positioned centrally, not covering any peripheral area of the gold cathode. Do not stretch the gasket across the POS-sensor head. One pack (4 seal tips) of this product is a standard component of the OroboPOS-Service Kit.26110-01
POS seal tip.jpg
OroboPOS-MembranesOroboPOS-Membranes, FEP 25 µm; 40/Pck. Two packages of this item are standard components of the OroboPOS-Service Kit.26200-01
POS membranes.jpg
OroboPOS-Electrolyte PowderOroboPOS-Electrolyte Powder, KCl.

This item is a standard component of the OroboPOS-Service Kit.

The powder is dissolved in 10 ml distilled water to yield a 1 M KCl solution.
26300-01
POS Electrolyte powder.jpg
OroboPOS-Mounting ToolOroboPOS-Mounting Tool for application of OroboPOS-Membranes. It consists of two parts, (i) the membrane guide (larger diameter) and (ii) the membrane ring holder with O-ring\Viton\6x1 mm for holding the OroboPOS-Membrane Ring during membrane application. This item is a standard component of the OroboPOS-Service Kit.26400-02
POS mounting tool for membrane application 02.JPG
OroboPOS-Polishing ClothOroboPOS-Polishing Cloth for cathode cleaning. Replace the OroboPOS-Polishing Cloth at intervals. A two-year interval may be considered in cases of intensive use. This item is a standard component of the OroboPOS-Service Kit.26510-01
POS polishing cloth for cathode cleaning.jpg
OroboPOS-Polishing PowderOroboPOS-Polishing Powder for cathode cleaning. This item is a standard component of the OroboPOS-Service Kit.26520-01
POS polishing powder for cathode cleaning.jpg
O2-Zero PowderO2-Zero Powder, dithionite (Na2S2O4), for zero-oxygen calibration of the OroboPOS. This item is a standard component of the OroboPOS-Service Kit.26600-01
O2-Zero Powder.jpg
Forceps for membrane applicationForceps for membrane application: for OroboPOS and ISE membrane application; do not use for tissue preparation. This item is a standard component of the OroboPOS-Service Kit and the O2k-TPP+ ISE-Module.26700-01
Forcep for membrane application.jpg
Pipette\Plastic\1 ml ungradedPipette\Plastic\1 mL ungraded, for filling electrolyte into the reservoir of the OroboPOS. This item is a standard component of the OroboPOS-Service Kit.26800-01
Pipette Plastic 1 ml-ungraded.JPG
ISS-Waste BottleISS-Waste Bottle, 2-liter, component of the ISS-Integrated Suction System.28120-01
ISS-Waste Bottle.JPG
ISS-LidISS-Lid, for ISS-Waste Bottle, component of the ISS-Integrated Suction System.28130-01
ISS-Lid.JPG
ISS-Filter and TubingISS-Filter and Tubing, ISS-Integrated Suction System.28140-01
ISS-Filter and Tubing.JPG
Power O2k NumbersPower O2k Numbers: Single number to label the O2k in a Power O2k-Lab.28200-01
Power O2k Numbers.JPG
O2k-Catalogue: TIP2k3#### O2k-Catalogue: TIP2k3####3####
Titration-Injection microPumpTitration-Injection microPump (TIP2k) for two-channel operation with the O2k with automatic control by DatLab of programmable titration regimes and feedback control (oxystat, pH-stat). Included in the TIP2k-Module.31000-01
Titration-Injection-microPump.jpg
Microsyringe\500 mm3\TIP2kMicrosyringe\500 mm3\TIP2k: Microinjection syringe for Titration-Injection microPump, 500 mm3 (µl), fixed injection needle with rounded tip, with spacers.31210-01
Microsyringe 500 mm3 TIP2k.JPG
Microsyringe\200 mm3\TIP2kMicrosyringe\200 mm3\TIP2k: Microinjection syringe for Titration-Injection microPump, 200 mm3 (µl), fixed injection needle with rounded tip, with spacers. Four units of this item are standard components of the TIP2k-Module.31220-01
Microsyringe200 mm3TIP2k.JPG
TIP2k-Needle SpacerTIP2k-Needle Spacers for microinjection TIP2k needle, silicone stops (200/Pkg.) with mounting tool. This item is a standard component of the TIP2k-Module.31310-01
80600-24 Spacers f. TIP2k needle.JPG
TIP2k Filter PapersTIP2k Filter Papers (package of 10) to be used for the stoppers during TIP2k titration for Instrumental oxygen background correction to avoid constant manual siphoning off of extruded liquid. One package is a standard component of the TIP2k-Module.31320-01
TIP2k Filter Papers.JPG
TIP2k-Needle Safety SupportTIP2k-Needle Safety Support: for safe storage of TIP2k-needles when not required during the experiment. This item is a standard component of the TIP2k-Module.31330-01
TIP2k-Needle Safety Support overview.JPG
O2k-Catalogue: O2k-Modules4#### O2k-Catalogue: O2k-Modules. O2k-Modules can be obtained with the O2k or added later, and can be simply installed by the user.4####4####
O2k-Catalogue: TPP+ ISE-Module42### O2k-Catalogue: TPP+ ISE-Module42###42###
ISE Package 1 TPP or CaO2k-TPP+ and Ca2+ ISE\1 Chamber: ISE-Package for 1 TPP+ and Ca2+ electrode.42010-01
ISE Package 1 TPP or Ca.JPG
Stopper\black PEEK\angular Shaft\side+6.2+2.6 mm PortStopper\black PEEK\angular Shaft\side+6.2+2.6 mm Port, for application with ISE; side titration port and two additional holes (6.2 mm and 2.6 mm); angular bottom; including Volume-Calibration Ring (A or B); 2 mounted O-rings, with 8 spare O-rings (O-ring\Viton\12x1 mm). Two stoppers are included in O2k-TPP+ ISE-Module.42100-01
Stopper black PEEK angular Shaft side+6.2+2.6 mm Port.JPG
Stopper-NeedleStopper-Needle: Short needle for bubble extrusion from port of the ISE-stopper.42120-01
Stopper-Needle.JPG
Ion-Selective Electrode TPP+ and Ca2+Ion-Selective Electrode TPP+ and Ca2+: ISE with 6 mm outer diameter shaft, for Stopper\white PVDF\angular Shaft\side+6.2+2.6 mm Port. O2k-TPP+ ISE-Module: 2 ISE.42200-01
Ion-Selective Electrode TPP+ and Ca2+.JPG
ISE-Inner Glass ElectrodeISE-Inner Glass Electrode of ISE, with Ag/AgCl- and Pt-wire42212-01
ISE-Inner Glass Electrode.JPG
ISE-Membrane SealISE-Membrane Seal for Ion-Selective Electrode TPP+ and Ca2+.42213-01
ISE-Membrane Seal.JPG
ISE-Compressible TubeISE-Compressible Tube for Ion-Selective Electrode TPP+ and Ca2+.42214-01
ISE-Compressible Tube.JPG
ISE-Consumables Spares SetISE-Consumables Spares Set42250-01
ISE-Consumables Spares Set.JPG
ISE-TPP+ MembranesISE-TPP+ Membranes, PVC, 4 mm diameter, box of 5 membranes. O2k-TPP+ ISE-Module: 2 boxes per electrode.42270-01
ISE-TPP+ Membranes.JPG
ISE-Ca2+ MembranesISE-Ca2+ Membranes: PVC, 4 mm diameter, box of 5 membranes. To be used with the O2k-TPP+ ISE-Module. The ISE-Ca2+ Membranes have to be ordered separately.42280-01
Ca2+ membranes.jpg
ISE-Membrane Mounting ToolISE-Membrane Mounting Tool for Ion-Selective Electrode TPP+ and Ca2+. O2k-TPP+ ISE-Module: mounting tool included.42300-01
ISA-Membrane Mounting Tool.JPG
ISE-Filling SyringeISE-Filling Syringe with needle for Ion-Selective Electrode TPP+ and Ca2+.42310-01
ISE-Filling Syringe.JPG
Reference-Electrode\2.4 mmReference-Electrode\2.4 mm: 2.4 mm diameter glass shaft, for ISE.42400-01
Reference Electrode 2.4 mm.jpg
Replacement-BarrelReplacement-Barrel for Reference-Electrode\2.4 mm, 2.4 mm diameter glass.42401-01
Replacement-Barrel.jpg
Electrolyte\Reference-ElectrodeElectrolyte\Reference-Electrode for Reference-Electrode\2.4 mm42402-01
Electrolyte Reference-Electrode.jpg
O2k-Catalogue: pH-Electrode43### O2k-Catalogue: pH-Electrode43###43###
Stopper\black PEEK\conical Shaft\central+2.3+2.6 mm PortStopper\black PEEK\conical Shaft\central+2.3+2.6 mm Port: for pH and reference electrode, central titration port and two additional ports (2.3 mm and 2.6 mm); conical bottom; including Volume-Calibration Ring (A or B), 2 mounted O-rings, with 8 spare O-rings (O-ring\Viton\12.5x1 mm). Two stoppers are included in the O2k-pH ISE-Module.43100-01
Stopper black PEEK conical Shaft central+2.3+2.6 mm Port.JPG
PH-Glass electrodepH-Glass electrode with BNC plug. Two pH electrodes are included in the O2k-pH ISE-Module.43200-01
PH-Glass Electrode.JPG
O2k-Catalogue: O2k-Fluorescence44### O2k-Catalogue: O2k-Fluorescence44###44###
Fluorescence-SensorFluorescence-Sensor: LED with specified wavelength, photodiode, Filter-Cap attached with specific optical filter for the LED and/or photodiode. See Fluorescence-Sensor Green and Fluorescence-Sensor Blue.442##
Fluorescence-Sensor.jpg
Fluorescence-Sensor GreenFluorescence-Sensor Green: excitation LED 525 nm (dominant wavelength), photodiode, Filter-Cap equipped with Filter Set AmR for Amplex UltraRed measurements when delivered. Two units of this item are standard components of the O2k-Fluo LED2-Module.44210-01
Fluorescence-Sensor Green.JPG
Fluorescence-Sensor BlueFluorescence-Sensor Blue: excitation LED 465 nm (dominant wavelength), photodiode, Filter-Cap equipped with Filter Set Saf for measurement of mitochondrial membrane potential with Safranin when delivered. Filter sets for Magnesium green® / Calcium green® measurements are included. Two units of this item are standard components of the O2k-Fluo LED2-Module.44220-01
Fluorescence-Sensor Green.JPG
Filter-CapFilter-Cap: O2k-Fluo LED2-Module (O2k-Series D to G) sensors (Fluorescence-Sensor Green and Fluorescence-Sensor Blue) and O2k-FluoRespirometer (O2k-Series H) sensors (Smart Fluo-Sensor Green and Smart Fluo-Sensor Blue) are equipped with a removable Filter-Cap for exchange of optical filters for the optical pathways from the LED to the sample and from the sample to the photodiode.44310-01
Filter-Cap.JPG
Filter Set AmRFilter Set AmR: Set of filters for the determination of H2O2 production with Amplex UltraRed. These filters should be used together with Fluorescence-Sensor Green. The filter set consists of 6 LED filters (round) and 6 photodiode filters (rectangular). One pair of these filters is attached to Fluorescence-Sensor Green when delivered as part of the O2k-Fluorescence LED2-Module. One filter set is a standard component of the O2k-Fluorescence LED2-Module and O2k-FluoRespirometer.44321-01
Filter Set AmR.JPG

AmR excitation scan 1mA.png

Excitation spectrum obtained with the Fluorescence-Sensor Green at 1mA current supply and equipped with the Filter Set AmR.
Filter Set SafFilter set Saf: Set of filters for the (qualitative) determination of mitochondrial membrane potential with Safranin. These filters should be used together with Fluorescence-Sensor Blue. The filter set consists of 6 LED filters (round) and 6 photodiode filters (rectangular). One pair of these filters is attached to Fluorescence-Sensor Blue when delivered as part of the O2k-Fluorescence LED2-Module.


One filter set is a standard component of the O2k-Fluorescence LED2-Module and O2k-FluoRespirometer.
44322-01
Filter Set Saf.JPG

Saf excitation scan 1mA.png

Excitation spectrum obtained with the Fluorescence-Sensor Blue at 1mA current supply and equipped with the Filter Set Saf.
Filter Set MgG / CaGFilter set MgG / CaG: Set of filters for the determination of concentraions of Mg2+ or Ca2+ with the fluorophores Magnesium green and Calcium green, respectively. These filters should be used together with Fluorescence-Sensor Blue. The filter set consists of 6 LED filters (round) and 6 photodiode filters (rectangular). One filter set is a standard component of the O2k-Fluorescence LED2-Module and O2k-FluoRespirometer.44323-01
Filter Set MgG CaG.JPG

MgG excitation scan 1mA.png

Excitation spectrum obtained with the Fluorescence-Sensor Blue at 1mA current supply and equipped with the filter set for Magnesium green® / Calcium green®.
O2k-Catalogue: NO-Amp-Module46### O2k-Catalogue: NO-Amp-Module46###46###
Stopper\black PEEK\conical Shaft\central Port\peripheral 2.3 mm PortStopper\black PEEK\conical Shaft\central+peripheral 2.3 mm Port: for potentiometric O2k-MultiSensor applications (NO, H2S, H2O2); 1 additional port (2.3 mm); central titration port; conical bottom; including Volume-Calibration Ring (A or B); 2 mounted O-rings, with 8 spare O-rings (O-ring\Viton\12.5x1 mm). O2k-MultiSensor: two stoppers in O2k-NO Amp-Module.46100-01
Stopper black PEEK conical Shaft central Port peripheral 2.3 mm Port.JPG
O2k-Catalogue: O2k-Accessories and Auxiliary Tools5#### O2k-Catalogue: O2k Accessories and Auxiliary Tools5####5####
O2k-Catalogue: Titration-Tools51### O2k-Catalogue: Titration-Tools51###51###
Microsyringe\10 mm3 51/0.13 mmHamilton Microsyringe\10 mm3 51/0.13 mm for manual titrations, 10 mm3 volume; fixed injection needle with rounded tip: 51 mm length, 0.13 mm inner diameter. O2k-FluoRespirometer and Startup O2k-Respirometer: 6 included in the O2k-Titration Set.51010-01
Hamilton Syringes for Manual Titration.jpg
Plunger\10 mm3Plunger\10 mm3 for Microsyringe\10 mm3 51/0.13 mm, spare. O2k-FluoRespirometer and Startup O2k-Respirometer: 3 in O2k-Titration Set.51011-01
Plunger 10 mm3.JPG
Microsyringe\25 mm3 51/0.15 mmHamilton Microsyringe\25 mm3 51/0.15 mm for manual titrations, 25 mm3 volume; fixed needle with rounded tip: 51 mm length, 0.15 mm inner diameter. O2k-FluoRespirometer and Startup O2k-Respirometer: 6 in O2k-Titration Set.51025-01
Microsyringe 25 mm3 51 0.15 mm.JPG
Microsyringe\50 mm3 51/0.15 mmHamilton Microsyringe\50 mm3 51/0.15 mm for manual titrations, 50 mm3 volume; fixed needle with rounded tip: 51 mm length, 0.15 mm inner diameter. O2k-FluoRespirometer and Startup O2k-Respirometer: 2 in O2k-Titration Set.51050-01
Microsyringe 100 mm3 51 0.41 mm - Kopie.JPG
Microsyringe\100 mm3 51/0.41 mmHamilton Microsyringe\100 mm3 51/0.41 mm for manual titrations, 100 mm3 volume; fixed needle with rounded tip: 51 mm length, 0.41 mm inner diameter. It is recommended for injections of suspensions of isolated mitochondria. O2k-FluoRespirometer and Startup O2k-Respirometer: 1 included in the O2k-Titration Set.51100-01
Microsyringe 100 mm3 51 0.41 mm - Kopie.JPG
Syringe\500 mm3 51/0.41 mmHamilton Syringe\500 mm3 51/0.41 mm for manual titrations, 500 mm3 volume; fixed needle with rounded tip: 51 mm length, 0.41 mm inner diameter; for injections of suspensions of isolated mitochondria and filling of the Microsyringe\200 mm3\TIP2k. O2k-FluoRespirometer and Startup O2k-Respirometer: 1 included in the O2k-Titration Set.51500-01
Syringe 500 mm3 51 0.41 mm.JPG
Syringe RacksSyringe Racks: stainless steel; for proper placement of eight Hamilton microsyringes in HRR experiments; package of two (for a total of 16 microsyringes). One package of two Syringe Racks and two Tube Racks is a standard component of the O2k-Titration Set.51610-01
Syringe Racks.JPG
Syringe CollarsSyringe Collars (package of 20) for Hamilton microsyringes: for correctly storing the microsyringes in the Syringe Racks. One package is a standard component of the Syringe Racks.51611-01
Syringe Collars.JPG
Tube RacksTube Racks: stainless steel, accomodating four 50-ml tubes, for cleaning of microsyringes, cleaning and storage of stoppers during cleaning of the O2k-Chambers; package of two (for a total of eight tubes). One package of two Tube Racks and two Syringe Racks is a standard component of the O2k-Titration Set.51620-01
Tube Racks.JPG
Syringe Storage BoxSyringe Storage Box: for storing the Hamilton microsyringes; incl. Syringe Labels, MiPNet19.14 SOP Hamilton microsyringes and MiPNet09.12 O2k-Titrations. One item is a standard component of the O2k-Titration Set.51630-01
Syringe Storage Box.jpg
Syringe LabelsSyringe Labels: set of labels with standard abbreviations (see O2k-Titrations). One set of labels is a standard component of the Syringe Storage Box.51631-02
Syringe Labels 2017.JPG
Titration Set UpgradeThe Titration Set Upgrade consists of the new items of the O2k-Titration Set for upgrading your original set (consisting only of Hamilton microsyringes). The upgrade consists of the Syringe Storage Box with Syringe Labels, a set of two Syringe Racks with Syringe Collars, and a set of two Tube Racks including tubes as well as an additional syringe (1 x 100 mm3).51910-01
O2k-Titration Set.JPG
Email: instruments@oroboros.at
O2k-Catalogue: PBI-Shredder52### O2k-Catalogue: PBI-Shredder52###
PBI-Shredder HRR-Set.JPG
PBI-Shredder SG3PBI-Shredder SG3 for tissue homogenate preparation, heavy duty high torque SG3 driver with convertible handle, SG3 base with 3 position force setting lever (FSL), battery charger and two lithium ion batteries. The PBI-Shredder SG3 is included in the PBI-Shredder O2k-Set. Select 230 V or 120 V. Oroboros Instruments: world-wide distributor.52100
PBI-Shredder SG3.jpg
Shredder-TubesShredder-Tubes: consisting of Shredder Tube FT500-PS with Lysis Disk, serrated Shredder-Ram and Shredder-Screw Cap, coral colour (Box of 100) and Shredder Tube FT500-PMS with Lysis Disk, serrated Shredder-Ram and Shredder-Screw Cap, coral colour (Box of 100). There is no difference between the white and the coral shredder tubes, except that the white tubes are also approved for high pressure. 1 box of each is included in the PBI-Shredder HRR-Set.52210-01, 52220-01
Shredder-Tube Analyt Biochem.jpg
O2k-Catalogue: Microbalance Mettler-Toledo53### O2k-Catalogue: Microbalance Mettler-Toledo53###
Microbalance Set.jpg
Microbalance 120 gMicrobalance 41 g to 120 g; 0.01 mg / 0.1 mg display; particularly for wet weight determination of permeabilized fibres.53100-01
Microbalance 120 g.jpg
Microbalance-Transport CaseMicrobalance transport case.53300-01
Microbalance-Transport Case.jpg
O2k-Catalogue: O2k-Dissection Tools54### O2k-Catalogue: O2k-Dissection Tools54###54###
Forceps\stainless Steel\straight Tip\sharpForceps\stainless Steel\straight Tip\sharp: for tissue preparation, stainless steel, antimagnetic. One pair is recommended for insertion of the sample into the O2k-Chamber and for handling in combination with Forceps\stainless Steel\rounded Tip\sharp. Set: in O2k-Dissection Set and PBI-Shredder O2k-Set.54210-01
Forcep for tissue preparation straight tip.jpg
Forceps\stainless Steel\rounded Tip\sharpForceps\stainless Steel\reounded Tip\sharp: for tissue preparation, stainless steel, antimagnetic. One pair is recommended for placing the tissue sample onto the microbalance and for handling in combination with Forceps\stainless Steel\straight Tip\sharp. Set: in O2k-Dissection Set.54220-01
Forcep for tissue preparation rounded tip.jpg
Forceps\stainless Steel\angular Tip\fineForceps\stainless Steel\angular Tip\fine: for tissue preparation, stainless steel. Two pairs are used particularly for muscle fibre separation. Set: 2 in O2k-Dissection Set.54230-01
Forcep for tissue preparation angular tip.jpg
Scissors\stainless steel\straight Tip\sharpScissors\stainless steel\straight Tip\sharp, included in PBI-Shredder O2k-Set and O2k-Dissection Set.54310-01
Scissors stainless steel straight Tip sharp.jpg
Motic MicroscopeMotic Microscope SMZ-171 TP: for preparation of permeabilized fibres; compact and light stereo microscope, Greenough optical system, switching power supply for use worldwide (100-240V); including auxiliary ESD objective 2.0X(38.6mm).54500-01
Motic Microscope.jpg
MiR05-KitMitochondrial Respiration Medium - MiR05-Kit, 1 vial; for a final volume of 250 mL60101-01
MiR05-Kit.jpg
MitoKit-CIICell permeable compound - MitoKit-CII, 2 vials; 1 vial of plasma membrane-permeable succinate-nv (Snv; NV118; 25 mg) and 1 vial of plasma membrane-permeable malonate-nv (Mnanv; NV161; 10 mg).60200-01
MITOKIT-CII.jpg
O2k-WorkshopsOroboros O2k-Workshops are organized as international oxygraph courses (IOC) on high-resolution respirometry since 1991. These range from four full-day training courses to conference lunch presentations of the Oroboros O2k and particularly the O2k-FluoRespirometer.7####
O2k-Workshops.png
MitoFit Coaching Days Innsbruck
MitoFit Laboratory

By organizing MitoFit Coaching Days Innsbruck, the Oroboros team responds to increasing demands for training in high-resolution respirometry (HRR) beyond the capacity of our regular O2k-Workshops. MitoFit Coaching Days are offered in the Oroboros MitoFit Laboratory according to demand for four to six participants (or labs). Experts provide training for quality control in high-resolution respirometry, aimed at standardization of SUIT protocols and fostering proficiency to generate experimental data of assured high quality as required for a data base on mitochondrial physiology.

The flexible programme is adjusted to the individual requirements of the participants, with specific coaching blocks particularly on instrumental setup and maintenance, instrumental quality control, MitoFit proficiency testing using standardized mitochondrial reference samples, DatLab analysis, SUIT protocols and experimental design, normalization of flux and data reporting, O2k-Fluorometry for measurement of hydrogen peroxide, measurement of mitochondrial membrane potential with fluorometric and potentiometric methods, measurement of respiration and acidification, quality control management in HRR. The MitoFit Coaching Days help participants to get prepared for their projects on HRR, even if the regular O2k-Workshops are fully booked.
7####
O2k-Workshops.png
O2k-Workshop\4 Days\StandardO2k-Workshop\4 Days\Standard: introductory O2k-Workshop and training course (4 Days), standard registration, basic or advanced. Module: A registration fee is waived in conjunction with the purchase of the Oroboros O2k, valid during the O2k-Warranty Period.70004-01
O2k-Workshop\4 Days\StudentO2k-Workshop\4 Days\Student: introductory training course (4 Days), student registration, basic or advanced. Module: A registration fee is waived in conjunction with the purchase of the Oroboros O2k, valid during the O2k-Warranty Period.70004-02
MitoFit Coaching Days - BasicMitoFit Coaching Days - Basic: individual introductory training course (3 Days), standard registration, 4-6 participants. One registration fee is waived in conjunction with the purchase of the Oroboros O2k (academic discount), valid during the O2k-Warranty Period.70020-01
MitoFit Proficiency TestThe MitoFit Proficiency Test for high-resolution respirometry includes the user-friendly software DatLab 7.4 and the Oroboros Premium Customer Support. Upon successful completion of the test, a MitoFit Proficiency Certificate will be conferred from Oroboros Instruments acknowledging your personal proficiency in your laboratory environment. The standard package includes the MiR05-Kit and cryopreserved HEK 293T cells for the Proficiency Test for up to three participants from the same research group.70050-01
Workshop 3 days StandardO2k-Workshop (3 days), standard registration.71003-01
Workshop 3 days StudentO2k-Workshop (3 days), student registration.71003-02
Workshop 7 days StandardO2k-MultiSensor Workshop (7 Days), standard registration; combined introductory and advanced O2k-MultiSensor Workshop.71007-01
Workshop 7 days StudentO2k-Multisensor-Workshop (7 Days), student registration; combined introductory and advanced O2k-MultiSensor Workshop.71007-02
O2k start-up serviceO2k start-up service, O2k training course (3 days). In most cases, the O2k-Manual provides sufficient detail for setting up the O2k and starting measurements, particularly in conjunction with participation in an introductory O2k-Workshop. For specific requirements, however, a start-up visit by a scientific application specialist supports a highly efficient start of a project.72003-01
O2k scientific support serviceO2k scientific support visit (4 days) by an O2k-application specialist provides scientific support in specific areas of applications, including help with sample preparation, design and testing of titration protocols (including SUIT protocols), and DatLab analysis.72004-01
OroboPOS-Connector ServicePOS connector service; replacement of cable and amplifier in the OROBOROS electronics workshop (WGT).80030-01
PR-Accessories98### PR-Accessories98###98###
T-Shirt: MitoFitT-Shirt MitoFit: Oroboros Logo on front, MitoFit Logo on back.98000-01
Tshirt MitoFit OROBOROS.jpg
T-Shirt: Oroboros black/organic cottonT-Shirt Oroboros black/organic cotton: An Oroboros on the front.98010-01
Packing99### Packing99###99###
Packing\O2k-Box 1Packing\O2k-Box 1: for shipment of the O2k-Main Unit, with polystyrene inlets. Keep the original packing material safely stored, for any future shipping purposes of the O2k-Main Unit.99010-01
Packing O2k-Box1.JPG
Packing\Peli CasePacking\Peli Case: Watertight, crushproof, and dust proof case for safe transportation of the O2k:

Retractable extension handle, Strong polyurethane wheels with stainless steel bearings, Easy open Double Throw latches, Open cell core with solid wall design - strong, light weight, O-ring seal, Automatic Pressure Equalization Valve, Fold down handles, Stainless steel hardware and padlock protectors, 4 level Pick N Pluck™ with convoluted lid foam, Special foam inlets for O2k.

Interior Dimensions: 21.48" x 16.42" x 12.54" (54.5 x 41.7 x 31.8 cm)
99012-01
Packing Peli Case.jpg
Packing\O2k-Box 2Packing\O2k-Box 2, for shipment of accessories.99020-01
Packing O2k-Box2.JPG
Oroboros support
O2k-Open Support
This information is part of O2k-Open Support.
O2k-Concept
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