Orynbayeva 2015 Nanomedicine (Lond)
|Orynbayeva Z, Sensenig R, Polyak B (2015) Metabolic and structural integrity of magnetic nanoparticle-loaded primary endothelial cells for targeted cell therapy. Nanomedicine (Lond) 10:1555-68.|
Abstract: Aim: To successfully translate magnetically mediated cell targeting from bench to bedside, there is a need to systematically assess the potential adverse effects of magnetic nanoparticles (MNPs) interacting with ‘therapeutic’ cells. Here, we examined in detail the effects of internalized polymeric MNPs on primary rat endothelial cells’ structural intactness, metabolic integrity and proliferation potential.
Materials & methods: The intactness of cytoskeleton and organelles was studied by fluorescent confocal microscopy, flow cytometry and high-resolution respirometry. Results: MNPloaded primary endothelial cells preserve intact cytoskeleton and organelles, maintain normal rate of proliferation, calcium signaling and mitochondria energy metabolism.
Conclusion: This study provides supportive evidence that MNPs at doses necessary for targeting did not induce significant adverse effects on structural integrity and functionality of primary endothelial cells – potential cell therapy vectors.
• Keywords: Ca2+ signaling, Cytoskeleton, Magnetic nanoparticles, Mitochondria membrane potential, Mitochondria oxidative phosphorylation, Primary endothelial cells
• O2k-Network Lab: US PA Philadelphia Orynbayeva Z
Labels: MiParea: Respiration, mt-Membrane
Organism: Rat Tissue;cell: Endothelial;epithelial;mesothelial cell Preparation: Intact cells
Coupling state: LEAK, ROUTINE, ET Pathway: ROX HRR: Oxygraph-2k