Ouabain

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Bioblasts - Richard Altmann and MiPArt by Odra Noel
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Ouabain

Description

Ouabain (synonym: G-strophantin octahydrate) is a poisonous cardiac glycoside. The classical mechanism of action of ouabain involves its binding to and inhibition of the plasma membrane Na+/K+-ATPase (sodium pump) especially at the higher concentrations. Low (nanomolar and subnanomolar) concentrations of ouabain stimulate the Na-K-ATPase.

Abbreviation: Oua

Reference: MiPNet03.02

Application in HRR

Oua: Ouabain octahydrate (≥95% (HPLC), powder; C29H44O12*8H20); Sigma O 3125, store at R.T.; FW = 728.77.
Caution: Light sensitive and toxic!
Preparation of 10 mM stock solution (dissolved in H2O):
  1. Weigh 7.3 mg ouabain.
  2. Dissolve in 1 mL H2O.
  3. Divide into 0.2 mL portions or store in 2 mL glass vial and use subsamples during experiments to avoid contamination of the storage solution.
  4. Store in glass vials (recommendation) at -20 °C.



MitoPedia topics: Inhibitor