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Oxia |
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Description
Oxia - HyperOxia to HypOxia: The Oxia generates gaseous oxygen and hydrogen by electrolysis of water using a proton exchange membrane (PEM). O2 and H2 gas can be used to control the O2 regime in the Oroboros O2k (Setting_the_oxygen_concentration). Low oxygen concentrations (<50 µM) are used to mimic tissue normoxia or hypoxia. Hyperoxic conditions above air saturation (250-600 µM O2) are routinely used for high-resolution respirometry of permeabilized muscle fibers or to induce oxidative stress in cells and mitochondrial preparations.
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Additional resources
MitoPedia O2k and high-resolution respirometry: O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry
