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MitoPedia O2k and high-resolution respirometry: DatLab
O2k-MultiSensor calibration - general
Select a MultiSensor plot and press F5 to open the calibration window, or select the channel from the Calibration menu, or press the pX (or Amp) buttons in the O2k-status line (e.g. left double click on pX calib.).
Select a channel and edit Channel label and Unit. In the pX calibration window a drop-down menu allows to select 1, 10^-3, 10^-6 or 10^-9 as Unit, in the Amp calibration window mM, µM or nM can be selected.
Calibrate: Apply changes (recalculates Signal and Slope).
Cancel: Press cancel to exit the calibration window without saving any changes.
pX channel: two-point calibration - DatLab
Linear calibration of pH as a function of recorded voltage is performed by a two-point calibration, using two pH calibration buffers, pX0 and pX1 (for example, pH0 is 7.0 and pH1 is 4.0; consider the actual pH of calibration buffers at experimental temperature).
1. Add pH calibration buffer 4.0 into respiration chamber, close chamber and insert pH electrode, record data, mark a section of the data when the signal is stable, name Mark pH 4; wash chamber.
2. Add pH calibration buffer 7.0 into respiration chamber, close chamber and insert pH electrode, record data, mark a section of the data when the signal is stable, name Mark pH 7.
3. Select the corresponding two marks in the "Select Mark" column.
4. Press Calibrate.
Note: for the calculation of proton fluxes you need to convert pH changes into proton concentrations. At present this needs to be done in a spreadsheet programm, i.e. outside of DatLab.