Paeaesuke 2015 Oxid Med Cell Longev

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Pääsuke R, Eimre M, Piirsoo A, Peet N, Laada L, Kadaja L, Roosimaa M, Pääsuke M, Märtson A, Seppet E, Paju K (2015) Proliferation of human primary myoblasts is associated with altered energy metabolism in dependence of ageing in vivo and in vitro. Oxid Med Cell Longev 8296150:10p.

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Paeaesuke R, Eimre M, Piirsoo A, Peet N, Laada L, Kadaja L, Roosimaa M, Paeaesuke M, Maertson A, Seppet E, Paju K (2015) Oxid Med Cell Longev

Abstract: Ageing is associated with suppressed regenerative potential of muscle precursor cells due to decrease of satellite cells and suppressive intramuscular milieu on their activation, associated with ageing-related low-grade inflammation. The aim of the study was to characterize the function of oxidative phosphorylation (OXPHOS), glycolysis, adenylate kinase (AK), and creatine kinase (CK)-mediated systems in young and older individuals.

Myoblasts were cultivated from biopsies taken by transcutaneous conchotomy from vastus lateralis muscle in young (20-29 yrs, n=7) and older (70-79 yrs, n=7) subjects. Energy metabolism was assessed in passages 2 to 6 by oxygraphy and enzyme analysis.

In myoblasts of young and older subjects the rate of OXPHOS decreased during proliferation from passage 2 to 6. The total activities of CK and AK decreased. Myoblasts of passage 2 cultivated from young muscle showed higher rate of OXPHOS and activities of CK and AK compared to myoblasts from older subjects while hexokinase and pyruvate kinase were not affected by ageing.

Proliferation of myoblasts in vitro is associated with down-regulation of OXPHOS and energy storage and transfer systems. Ageing in vivo exerts an impact on satellite cells which results in altered metabolic profile in favour of the prevalence of glycolytic pathways over mitochondrial OXPHOS of myoblasts.

Keywords: Human primary myoblasts, Ageing, Mitochondria, Adenylate kinase, Creatine kinase

O2k-Network Lab: EE Tartu Paju K, EE Tartu Seppet EK

Labels: MiParea: Respiration, mtDNA;mt-genetics  Pathology: Aging;senescence 

Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Permeabilized cells 

Coupling state: OXPHOS  Pathway: N, S, CIV, ROX  HRR: Oxygraph-2k