Palenickova 2011 Physiol Res

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Palenickova E, Cahova M, Drahota Z, Kazdova L, Kalous M (2011) Inhibitory effect of metformin on oxidation of NADH-dependent substrates in rat liver homogenate. Physiol Res 60:835-9.

» PMID: 21812516 Open Access

Palenickova E, Cahova M, Drahota Z, Kazdova L, Kalous M (2011) Physiol Res

Abstract: Metformin is widely used in the treatment of Type 2 diabetes, however, mechanisms of its antihyperglycemic effect were not yet fully elucidated. Complex I of mitochondrial respiration chain is considered as one of the possible targets of metformin action. In this paper, we present data indicating that the inhibitory effect of metformin can be tested also in liver homogenate. Contrary to previous findings on hepatocytes or mitochondria under our experimental conditions, lower metformin concentrations and shorter time of preincubation give significant inhibitory effects. These conditions enable to study the mechanism of the inhibitory effect of metformin in small samples of biological material (50-100 mg wet weight) and compare more experimental groups of animals because isolation of mitochonria is unnecessary.

Keywords: Liver homogenate, Glutamate and palmityl carnitine oxidation, Metformin

O2k-Network Lab: CZ Prague Houstek J, CZ Prague Kalous M


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Organism: Rat  Tissue;cell: Liver  Preparation: Homogenate, Isolated mitochondria 



HRR: Oxygraph-2k