Paulsen 2011 Cell Transplant

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Paulsen BD, Maciel RD, Galina A, da Silveira MS, Souza CD, Drummond H, Pozzato EN, Junior HS, Chicaybam L, Massuda R, Setti-Perdigão P, Bonamino M, Belmonte-de-Abreu PS, Castro NG, Brentani H, Rehen SK (2011) Altered oxygen metabolism associated to neurogenesis of induced pluripotent stem cells derived from a schizophrenic patient. Cell Transplant 21:1547-59

» PMID: 21975034

Paulsen BD, Maciel RD, Galina A, da Silveira MS, Souza CD, Drummond H, Pozzato EN, Junior HS, Chicaybam L, Massuda R, Setti-Perdigao P, Bonamino M, Belmonte-de-Abreu PS, Castro NG, Brentani H, Rehen SK (2011) Cell Transplant

Abstract: Schizophrenia has been defined as a neurodevelopmental disease that causes changes in the process of thoughts, perceptions and emotions, usually leading to a mental deterioration and affective blunting. Studies have shown altered cell respiration and oxidative stress response in schizophrenia; however, most of the knowledge has been acquired from post-mortem brain analyses or from non-neural cells. Here we describe that neural cells, derived from induced pluripotent stem cells generated from skin fibroblasts of a schizophrenic patient, presented a 2-fold increase in extra-mitochondrial oxygen consumption as well as elevated levels of reactive oxygen species (ROS), when compared to controls. This difference in ROS levels was reverted by the mood stabilizer valproic acid. Our model shows evidence that metabolic changes occurring during neurogenesis are associated with schizophrenia, contributing to a better understanding of the development of the disease and highlighting potential targets for treatment and drug screening.

Keywords: Schizophrenia, oxygen metabolism, induced pluripotent stem cells, ROS production

O2k-Network Lab: BR_Rio de Janeiro_Galina A


Labels:

Stress:Oxidative stress;RONS, Mitochondrial disease  Organism: Human  Tissue;cell: Fibroblast  Preparation: Intact cells 


Coupling state: OXPHOS 

HRR: Oxygraph-2k