Pereira da Silva Grilo da Silva F

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MiPsociety
COST Action CA15203 MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping


 

Pereira da Silva Grilo da Silva F


MitoPedia topics: EAGLE 

COST: Member


COST WG4: WG4

COST ECI: ECI


Name Pereira da Silva Grilo da Silva Filomena, PhD.
Institution
Filomena SGSilva.jpg
Center for Neuroscience and Cell Biology,

University of Coimbra, PT

Visiting scientist

  • Understanding mitochondrial remodeling in NAFLD

Filomena Pereira da Silva Grilo da Silva is a visiting scientist at Oroboros Instruments within the Secondment MtFOIE GRAS from October 2017 to February 2018 and in March 2019.

Address Biocant Park, 3060-197
City Catanhede
State/Province
Country Portugal
Email fsgsilv@gmail.com
Weblink
O2k-Network Lab


Labels:



Publications

 PublishedReference
Gnaiger 2019 MitoFit Preprint Arch2019Gnaiger E, Aasander Frostner E, Abdul Karim N, Abdel-Rahman EA, Abumrad NA, Acuna-Castroviejo D, Adiele RC, et al (2019) Mitochondrial respiratory states and rates. MitoFit Preprint Arch doi:10.26124/mitofit:190001.v5.

Abstracts

 PublishedReference
Silva 2019 ESCI20192019Silva FSG, Komlodi T, Garcia-Souza LF, Bento G, Doerrier C, Oliveira PJ, Gnaiger E (2019) Can fatty acid oxidation be specifically blocked by the CPT1 inhibitor etomoxir?
Silva 2019 MiPschool Coimbra2019
Filomena Pereira da Silva Grilo da Silva
Off-target effects of etomoxir on mitochondrial complex I.
Pereira da Silva Grilo da Silva 2018 MiP20182018
Filomena Pereira da Silva Grilo da Silva
The unspecific effect of etomoxir on mitochondrial respiration.
Silva 2017 MiP20172017
Pereira da Silva Grilo da Silva Filomena
No abstract.

MitoEAGLE Inclusiveness Target Countries - Conference Grant

Scientific report - ESCI2018

MitoEAGLE Short-Term Scientific Mission

Work Plan summary
1. The liver mitochondrial dysfunction plays an important role in the progression of non-alcoholic steatohepatitis, therefore improved protocols are required to evaluate mitochondrial fitness in the course of disease. Etomoxir is a well-known compound which irreversibly inhibits the carnitine palmitoyltransferase (CPT-1) in the outer surface of the mitochondrial inner membrane, therefore, blocks the transport of fatty acids into the mitochondrial matrix for further oxidation. However, among others we have shed light on the unspecific effect of etomoxir on the mitochondrial fatty acid oxidation (FAO; F-pathway linked respiration) in permeabilized Huh7 liver cells and mitochondria isolated from mouse liver. In our previous study (data not published) we have observed that the effect of etomoxir is concentration dependent. Low concentration (~40 µM) of etomoxir might inhibit NADH-linked respiration supported by glutamate or pyruvate plus malate. We have seen that high concentration of etomoxir (~200 µM) might block not only the NADH-linked pathway but also the Succinate-linked respiration.
The aim of the secondment is to adjust the optimal etomoxir concentration which does not exert inhibitory effect towards other respiratory pathways and specifically blocks FAO on mitochondria.
2. The goal of this secondment is to provide standardized protocols to study FAO and to deepen our knowledge about the effect of etomoxir on F-pathway linked mitochondrial respiration and get rid of the artefacts created by overdosage of the inhibitor.
Oroboros laboratory would provide a platform with its instrumental background, with its protocols, its experience and its international team to the objectives and aim of MitoEAGLE.
3. High-resolution Respirometry experiments will be performed on isolated mitochondria from mouse liver, heart and brain as well as permeabilized Huh7 liver cell lines. In order to detect FAO, we have used palmitoylcarnitine as source of fatty acid, which needs CPT-1 to enter the mitochondrial matrix and low concentration of malate supporting FAO. We also want to test other sources of fatty acid such as palmitoyl-CoA + carnitine + low amount of malate or palmitate + CoA + carnitine, which might influence the effect of etomoxir on CPT-1.

Participated at


Visiting scientist in the Oroboros MitoFit Laboratory

O2k-Network

Filomena Pereira da Silva Grilo da Silva: Visiting scientist at the Oroboros MitoFit Laboratory

  • March 04 to March 22 2019
  • October 01 2016 to February 28 2017