Permeabilized tissue (pti, see also permeabilized muscle fibers, pfi) are mitochondrial preparations obtained by selectively permeabilizing the plasma membrane mechanically or chemically (e.g., with saponin), for the exchange of soluble molecules between the cytosolic phase and external medium, without damaging the mt-membranes.
Addition of sample to the respirometer chamber: For permeabilized tissue, remove the Stopper, add the permeabilized tissue into the O2k-Chamber, close the chamber again.
|BEC 2020.1 doi10.26124bec2020-0001.v1||Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. doi:10.26124/bec:2020-0001.v1.||2020|
MitoPedia topics: Sample preparation