Perrin 2002 Biochimie
|Perrin A, Roudier E , Duborjal H, Bachelet C, Riva-Lavieille C, Leverve X, Massarelli R (2002) Pyruvate reverses metabolic effects produced by hypoxia in glioma and hepatoma cell cultures. Biochimie 84:1003-11.|
Abstract: The intervention of pyruvate in glucose metabolism was investigated during hypoxic stress in tumour cell cultures having respiratory capacities under normoxic conditions. Results obtained with nuclear magnetic resonance (NMR) spectroscopy showed that, under normoxic conditions, rat glioma C6 and human hepatoma Hep G2 cell cultures metabolised 13C1 glucose into lactate, alanine, glutamate and other less abundant metabolites, as already known from the literature. In the absence of pyruvate, during hypoxia or cyanide poisoning, both cell types dramatically decreased the label into glutamate and accumulated 13C3 glycerol-3-phosphate. The compound was further identified by 31P NMR spectroscopy. The accumulation of the label in glycerol-3-phosphate, however, did not occur when the cells were incubated in the presence of pyruvate. The fate of the latter, followed under normoxic conditions by incubating cells with 13C3 pyruvate and natural glucose, showed that the label was mainly found in alanine, lactate and glutamate. Anoxic conditions increased the label in lactate and reduced that of glutamate. The data show a metabolic effect of pyruvate during mitochondrial blockade due to severe lack of oxygen in tumour cell lines.
• Keywords: Glycerol-3-phosphate, Hypoxic conditions, Metabolism; Cyanide, 13C NMR
• O2k-Network Lab: FR Grenoble Saks VA
Labels: Pathology: Cancer Stress:Ischemia-reperfusion Organism: Human, Rat Tissue;cell: Nervous system, Liver Preparation: Intact cells