Pozenel 2019 Cells

From Bioblast
Jump to navigation Jump to search
Publications in the MiPMap
Pozenel L, Lindenmair A, Schmidt K, Kozlov AV, Grillari J, Wolbank S, Banerjee A, Weidinger A (2019) Critical impact of human amniotic membrane tension on mitochondrial function and cell viability in vitro. Cells 8:E1641.

» PMID: 31847452 Open Access

Pozenel L, Lindenmair A, Schmidt K, Kozlov AV, Grillari J, Wolbank S, Banerjee A, Weidinger A (2019) Cells

Abstract: Amniotic cells show exciting stem cell features, which has led to the idea of using living cells of human amniotic membranes (hAMs) in toto for clinical applications. However, under common cell culture conditions, viability of amniotic cells decreases rapidly, whereby reasons for this decrease are unknown so far. Recently, it has been suggested that loss of tissue tension in vivo leads to apoptosis. Therefore, the aim of this study was to investigate the effect of tissue distention on the viability of amniotic cells in vitro. Thereby, particular focus was put on vital mitochondria-linked parameters, such as respiration and ATP synthesis. Biopsies of hAMs were incubated for 7-21 days either non-distended or distended. We observed increased B-cell lymphoma 2-associated X protein (BAX)/B-cell lymphoma (BCL)-2 ratios in non-distended hAMs at day seven, followed by increased caspase 3 expression at day 14, and, consequently, loss of viability at day 21. In contrast, under distention, caspase 3 expression increased only slightly, and mitochondrial function and cellular viability were largely maintained. Our data suggest that a mechano-sensing pathway may control viability of hAM cells by triggering mitochondria-mediated apoptosis upon loss of tension in vitro. Further studies are required to elucidate the underlying molecular mechanisms between tissue distention and viability of hAM cells. Keywords: BAX, BCL-2, Apoptosis, Human amniotic membrane, Mitochondrial cell death, Tensile strength Bioblast editor: Plangger M O2k-Network Lab: AT Vienna Kozlov AV, IN Haldia Chakrabarti S


Labels: MiParea: Respiration 

Stress:Cell death  Organism: Human  Tissue;cell: Genital  Preparation: Intact cells 

Regulation: ATP production  Coupling state: ROUTINE 

HRR: Oxygraph-2k 

Labels, 2020-01