Quoilin 2012 Biochem Biophys Res Commun

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Quoilin C, Mouithys-Mickalad A, Duranteau J, Gallez B, Hoebeke M (2012) Endotoxin-induced basal respiration alterations of renal HK-2 cells: A sign of pathologic metabolism down-regulation. Biochem Biophys Res Commun 423:350-54.

» PMID: 22659746

Quoilin C, Mouithys-Mickalad A, Duranteau J, Gallez B, Hoebeke M (2012) Biochem Biophys Res Commun

Abstract: To study the mechanism of oxygen regulation in inflammation-induced acute kidney injury, we investigate the effects of a bacterial endotoxin (lipopolysaccharide, LPS) on the basal respiration of proximal tubular epithelial cells (HK-2) both by high-resolution respirometry and electron spin resonance spectroscopy. These two complementary methods have shown that HK-2 cells exhibit a decreased oxygen consumption rate when treated with LPS. Surprisingly, this cellular respiration alteration persists even after the stress factor was removed. We suggested that this irreversible decrease in renal oxygen consumption after LPS challenge is related to a pathologic metabolic down-regulation such as a lack of oxygen utilization by cells.

Keywords: Endotoxin, HK-2 cells, Basal respiration, LPS

O2k-Network Lab: BE Liege Votion DM


Tissue;cell: Endothelial;epithelial;mesothelial cell, Other cell lines  Preparation: Intact cells 

Regulation: Flux control  Coupling state: ROUTINE 

HRR: Oxygraph-2k