Quoilin 2012 Biochem Biophys Res Commun
|Quoilin C, Mouithys-Mickalad A, Duranteau J, Gallez B, Hoebeke M (2012) Endotoxin-induced basal respiration alterations of renal HK-2 cells: A sign of pathologic metabolism down-regulation. Biochem Biophys Res Commun 423:350-54.|
Abstract: To study the mechanism of oxygen regulation in inflammation-induced acute kidney injury, we investigate the effects of a bacterial endotoxin (lipopolysaccharide, LPS) on the basal respiration of proximal tubular epithelial cells (HK-2) both by high-resolution respirometry and electron spin resonance spectroscopy. These two complementary methods have shown that HK-2 cells exhibit a decreased oxygen consumption rate when treated with LPS. Surprisingly, this cellular respiration alteration persists even after the stress factor was removed. We suggested that this irreversible decrease in renal oxygen consumption after LPS challenge is related to a pathologic metabolic down-regulation such as a lack of oxygen utilization by cells.
• Keywords: Endotoxin, HK-2 cells, Basal respiration, LPS
• O2k-Network Lab: BE Liege Votion DM
Tissue;cell: Endothelial;epithelial;mesothelial cell, Other cell lines Preparation: Intact cells
Regulation: Flux control Coupling state: ROUTINE