Raboel 2010 J Clin Endocrinol Metab

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Raboel R, Larsen S, Hojberg PM, Almdal T, Boushel RC, Haugaard SB, Andersen JL, Madsbad S, Dela F (2010) Regional anatomic differences in skeletal muscle mitochondrial respiration in type 2 diabetes and obesity. J Clin Endocrinol Metab 95:857-63.

» PMID: 20061415

Raboel R, Larsen S, Hojberg PM, Almdal T, Boushel RC, Haugaard SB, Andersen JL, Madsbad S, Dela F (2010) J Clin Endocrinol Metabol

Abstract: Context: Previous studies on leg skeletal musculature have demonstrated mitochondrial dysfunction associated with type 2 diabetes mellitus (T2DM), but it is not known whether mitochondrial dysfunction is present in the upper extremities.

Objective: The aim of the study was to compare mitochondrial respiration and markers of mitochondrial content in skeletal muscle of arm and leg in patients with T2DM and obese control subjects.

Patients: Ten patients with T2DM (age, 52.3 ± 2.7 yr; body mass index, 30.1 ± 1.2 kg/m2) (mean ± SE) were studied after a 2-wk washout period of oral antihyperglycemic agents. Ten control subjects (age, 54.3 ± 2.8 yr; body mass index, 30.4 ± 1.2 kg/m2) with normal fasting and 2-h oral glucose tolerance test blood glucose levels were also included. Main Outcome Measure:Wemeasured mitochondrial respiration in saponin-treated skinned muscle fibers from biopsies of m. deltoideus and m. vastus lateralis using high-resolution respirometry.

Results: In the arm, mitochondrial respiration and citrate synthase activity did not differ between groups, but mitochondrial respiration per milligram of muscle was significantly higher in the leg muscle of the control subjects compared to T2DM. Fiber type compositions in arm and leg muscles were not different between the T2DM and control group, and maximum rate of O2 consumption did not differ between the groups.

Conclusion: The results demonstrate that reduced mitochondrial function in T2DM is only present in the leg musculature. This novel finding suggests that mitochondrial dysfunction is not a primary defect affecting all skeletal muscle but could be related to a decreased response to locomotor muscle use in T2DM. (J Clin Endocrinol Metab 95: 857–863, 2010)


O2k-Network Lab: CA Vancouver Boushel RC, DK Copenhagen Dela F, SE Stockholm Boushel RC, DK Copenhagen Larsen S


Labels: Pathology: Diabetes 

Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue 



HRR: Oxygraph-2k