Remels 2010 Mol Cell Endocrinol
|Remels AH, Langen RC, Schrauwen P, Schaart G, Schols AM, Gosker HR (2010) Regulation of mitochondrial biogenesis during myogenesis. Mol Cell Endocrinol 315:113-20.|
Abstract: Pathways involved in mitochondrial biogenesis associated with myogenic differentiation are poorly defined. Therefore, C(2)C(12) myoblasts were differentiated into multi-nucleated myotubes and parameters/regulators of mitochondrial biogenesis were investigated. Mitochondrial respiration, citrate synthase- and beta-hydroxyacyl-CoA dehydrogenase activity as well as protein content of complexes I, II, III and V of the mitochondrial respiratory chain increased 4-8-fold during differentiation. Additionally, an increase in the ratio of myosin heavy chain (MyHC) slow vs MyHC fast protein content was observed. PPAR transcriptional activity and transcript levels of PPAR-alpha, the PPAR co-activator PGC-1alpha, mitochondrial transcription factor A and nuclear respiratory factor 1 increased during differentiation while expression levels of PPAR-gamma decreased. In conclusion, expression and activity levels of genes known for their regulatory role in skeletal muscle oxidative capabilities parallel the increase in oxidative parameters during the myogenic program. In particular, PGC-1alpha and PPAR-alpha may be involved in the regulation of mitochondrial biogenesis during myogenesis.
• Keywords: Peroxisome, Proliferator-activated, Receptors, Myogenesis, Mitochondrial transcription factor A, Nuclear respiratory factor 1, Skeletal muscle, C2C12 cells, L6 muscle cells
• O2k-Network Lab: NL Maastricht Schrauwen P
Labels: MiParea: Respiration
Organism: Mouse, Rat Tissue;cell: Skeletal muscle, Other cell lines Preparation: Permeabilized cells
Coupling state: ROUTINE, OXPHOS, ET Pathway: F HRR: Oxygraph-2k