Remor 2011 Biochim Biophys Acta

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Remor AP, de Matos FJ, Ghisoni K, da Silva TL, Eidt G, Búrigo M, de Bem AF, Silveira PC, de León A, Sanchez MC, Hohl A, Glaser V, Saraiva-Gonçalves CA, Quincozes-Santos A, Borba Rosa R, Latini A (2011) Differential effects of insulin on peripheral diabetes-related changes in mitochondrial bioenergetics: Involvement of advanced glycosylated end products. Biochim Biophys Acta 1812:1460-71.

» PMID: 21767639 Open Access

Remor AP, de Matos FJ, Ghisoni K, da Silva TL, Eidt G, Burigo M, De Bem Andreza Fabro, Silveira PC, de Leon A, Sanchez MC, Hohl A, Glaser V, Saraiva-Goncalves CA, Quincozes-Santos A, Borba Rosa R, Latini A (2011) Biochim Biophys Acta

Abstract: Large scale clinical trials have demonstrated that an intensive antihyperglycemic treatment in diabetes mellitus (DM) in individuals reduces the incidence of micro- and macrovascular complications, e.g. nephropathy, retinopathy, DM-accelerated atherosclerosis, myocardial infarction, or limb amputations. Here, we investigated the effect of short- and long-term insulin administration on mitochondrial function in peripheral tissues of streptozotocin (STZ)-induced hyperglycemic rats. In addition, the in vitro effect of methylglyoxal (MG), advanced glycation end products (AGEs) and human diabetic plasma on mitochondrial activity was investigated in skeletal muscle and liver mitochondria and in rat skin primary fibroblasts. Hyperglycemic STZ rats showed tissue-specific patterns of energy deficiency, evidenced by reduced activities of complexes I, II and/or IV after 30 days of hyperglycemia in heart, skeletal muscle and liver; moreover, cardiac tissue was found to be the most sensitive to the diabetic condition, since energy metabolism was impaired after 10 days of the hyperglycemia. Insulin-induced tight glycemic control was effective in protecting against the hyperglycemia-induced inhibition of mitochondrial enzyme activities. Furthermore, the long-term hormone replacement (30 days) also increased these activities in kidney from STZ-treated animals, where the hyperglycemic state did not modify the electron transport activity. Results from in vitro experiments indicate that mitochondrial impairment could result from oxidative stress-induced accumulation of MG and/or AGEs. Further investigations demonstrated that human plasma AGE accumulation elicits reduced mitochondrial function in skin fibroblast. These data suggest that persistent hyperglycemia results in tissue-specific patterns of energy deficiency and that early and continuous insulin therapy is necessary to maintain proper mitochondrial metabolism.

Keywords: Hyperglycemia, Insulin, Mitochondrial oxidative stress, Cardioprotection, Streptozotocin

O2k-Network Lab: BR Florianapolis Latini A, BR Florianopolis De Bem AF


Labels: Pathology: Diabetes 

Organism: Rat  Tissue;cell: Endothelial;epithelial;mesothelial cell, Fibroblast  Preparation: Intact cells 



HRR: Oxygraph-2k 

diabetes