Respiratory state

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MitoPedia

Respiratory state

Description

Respiratory states of mitochondrial preparations and living cells are defined in the current literature in many ways and with a diversity of terms. Mitochondrial respiratory states must be defined in terms of both, the coupling-control state and the electron-transfer-pathway state.


Reference: Gnaiger 2014 MitoPathways


MitoPedia concepts: MiP concept, Respiratory state, SUIT concept, Recommended 


MitoPedia methods: Respirometry, Spectrophotometry 


MitoPedia topics: EAGLE 

Communicated by Gnaiger E 2010-10-21, edited 2016-08-26. Edited by Doerrier C 2020-04-23.
OXPHOS
ROUTINE
ETS
LEAK
ROX

Coupling control states

Coupling control states and CCR of mitochondrial preparations:
Coupling control states of living cells:

Electron-transfer-pathway state

Electron-transfer-pathway state, are defined by substrate type (at saturating concentration):
Control by substrate concentration: Kinetic control states:
  • Kinetic substrate or adenylate control: Kinetic studies with variation of a specific substrate (reduced substrate supplying electrons to the ETS; ADP, Pi; O2; cytochrome c) are analyzed by kinetic functions (e.g. hyperbolic), yielding apparent kinetic constants, such as Jmax, Km', c50, or p50.
  • Kinetic inhibitor control: Kinetic studies with variation of a specific inhibitor yield apparent kinetic constants, such as the KI'.

Classical respiratory states

Chance and Williams (1955):
Derived respiratory states:
Thermodynamics of irreversible processes:


MitoPedia: Respiratory states

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