Ruiz-Perez 2015 J Biol Chem
|Ruiz-Pérez MV, Medina MÁ, Urdiales JL, Keinänen TA, Sánchez-Jiménez F (2015) Polyamine metabolism is sensitive to glycolysis inhibition in human neuroblastoma cells. J Biol Chem 290:6106-19.|
Abstract: Polyamines are essential for cell proliferation, and their levels are elevated in many human tumours. The oncogene n-myc is known to potentiate polyamine metabolism. Neuroblastoma, the most frequent extra-cranial solid tumour in children, harbours the amplification of n-myc oncogene in 25% of the cases, and it is associated with treatment failure and poor prognosis. We evaluated several metabolic features of the human neuroblastoma cell lines Kelly, IMR-32 and SK-N-SH. We further investigated the effects of glycolysis impairment in polyamine metabolism in these cell lines. A previously unknown linkage between glycolysis impairment and polyamine reduction is unveiled. We show that glycolysis inhibition is able to trigger signalling events leading to the reduction of N-Myc protein levels and a subsequent decrease of both ornithine decarboxylase expression and polyamine levels, accompanied by cell cycle blockade preceding cell death. New anti-tumour strategies could take advantage of the direct relationship between glucose deprivation and PA metabolism impairment leading to cell death and its apparent dependence on n-myc. Combined therapies targeting glucose metabolism and PA synthesis could be effective in the treatment of n-myc-expressing tumours.
• Keywords: Cancer metabolism, Neuroblastoma, N-Myc, Polyamines, Glycolysis, Human neuroblastoma cells Kelly, IMR-32, SK-N-SH
• O2k-Network Lab: ES Malaga Medina MA
Labels: MiParea: Respiration Pathology: Cancer
Organism: Human Tissue;cell: Nervous system, Other cell lines, Neuroblastoma Preparation: Intact cells
Coupling state: LEAK, ROUTINE, ET Pathway: ROX HRR: Oxygraph-2k