|Step||Respiratory state||Pathway control||Comment|
|ce1||ROUTINE||Living cells (ce)||Dead cells (dce) without substrate are in a state of ROX.|
|ce1SD||R+SP||ROUTINE (R) + S (SP)||dce with functional mitochondria are in state SP.|
|ce2Omy||L+SL||LEAK (L) + S (SL)||ce and dce are in the LEAK state.|
|ce3U||E+SE||Electron transfer pathway (E) + S (SE)||ce and dce are in the ET state.|
|ce4Rot||ROX+SE||ROX+S (SE)||ce are in the ROX state, dce are in the ET state.|
- ce1;ce1S .. versus ce1;ce2Omy;ce3U;ce4S .. and respiration medium
- Living cells & succinate: ADP is not permeable through the plasma membrane of living cells, and this is the case for succinate in many cell types (Steinlechner-Maran 1997 Transplantation). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability (Stadlmann_2006_Cell Biochem Biophys).