Santos 2016 Insect Biochem Mol Biol

From Bioblast
Jump to: navigation, search
Publications in the MiPMap
Santos DE, Alberici LC, Hartfelder K (2016) Mitochondrial structure and dynamics as critical factors in honey bee (Apis mellifera L.) caste development. Insect Biochem Mol Biol 73:1-11.

» PMID: 27058771

Santos DE, Alberici Luciane Carla, Hartfelder K (2016) Insect Biochem Mol Biol

Abstract: The relationship between nutrition and phenotype is an especially challenging question in cases of facultative polyphenism, like the castes of social insects. In the honey bee, Apis mellifera, unexpected modifications in conserved signaling pathways revealed the hypoxia response as a possible mechanism underlying the regulation of body size and organ growth. Hence, the current study was designed to investigate possible causes of why the three hypoxia core genes are overexpressed in worker larvae. Parting from the hypothesis that this has an endogenous cause and is not due to differences in external oxygen levels we investigated mitochondrial numbers and distribution, as well as mitochondrial oxygen consumption rates in fat body cells of queen and worker larvae during the caste fate-critical larval stages. By immunofluorescence and electron microscopy we found higher densities of mitochondria in queen larval fat body, a finding further confirmed by a citrate synthase assay quantifying mitochondrial functional units. Oxygen consumption measurements by high-resolution respirometry revealed that queen larvae have higher maximum capacities of ATP production at lower physiological demand. Finally, the expression analysis of mitogenesis-related factors showed that the honey bee TFB1 and TFB2 homologs, and a nutritional regulator, ERR, are overexpressed in queen larvae. These results are strong evidence that the differential nutrition of queen and worker larvae by nurse bees affects mitochondrial dynamics and functionality in the fat body of these larvae, hence explaining their differential hypoxia response.

Copyright © 2016 Elsevier Ltd. All rights reserved.

Keywords: High-resolution respirometry, Honeybee, Hypoxia response, Mitochondrial transcription factors, Queen development

O2k-Network Lab: BR Ribeirao Preto Alberici LC

Labels: MiParea: Respiration, mt-Biogenesis;mt-density, nDNA;cell genetics, Comparative MiP;environmental MiP, Exercise physiology;nutrition;life style 

Stress:Oxidative stress;RONS  Organism: Hexapods  Tissue;cell: Fat  Preparation: Permeabilized tissue 

Coupling state: LEAK, OXPHOS, ET  Pathway:HRR: Oxygraph-2k