Save a DLD file. When disconnected from the O2k, save any changes made under the identical file name overwriting the previous file. Such changes do not affect the raw data of the experiment, but relate to calibrations, experimental protocol, marks, events, and layout.
Temporary backup files are generated by DatLab in the current user's temp directory, indicated by adding tmp.$$$ to the file name. These files are retained only if the PC has failed during data analysis. During data acquisition, the data are written continuously onto the file, hence backup files are not necessary under these conditions.
MitoPedia O2k and high-resolution respirometry: DatLab