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Schiffer 2013 Abstract MiP2013

From Bioblast
Schiffer TA, Weitzberg E, Lundberg JO, Larsen FJ (2013) Dietary inorganic nitrate reduces basal metabolic rate in man. Mitochondr Physiol Network 18.08.


Tomas Schiffer

MiP2013, Book of Abstracts Open Access

Schiffer TA, Weitzberg E, Lundberg JO, Larsen FJ (2013)

Event: MiPNet18.08_MiP2013

According to the rate-of-living and oxidative damage theory of aging, extended lifespan is predicted by low energy metabolism and low reactive oxygen species production rates. Recently, several studies show that dietary inorganic nitrate mainly present in vegetables can reduce oxygen consumption during physical exercise in humans [1] and contribute to attenuated oxidative stress in animal models of disease [2]. Nitrate accumulates in saliva and is bioactivated through reduction to nitrite by oral bacteria.

We examined the effects of dietary nitrate on basal metabolic rate (BMR) and markers of oxidative stress in man using a double-blind, randomized cross over design. 15 young healthy males volunteered and indirect calorimetry was used to determine basal metabolic rate after three days of dietary intervention with sodium nitrate (NaNO3, 0.1 mmol∙kg-1∙day-1) or placebo (NaCl). The administered amount of nitrate resembles what is found in 100-300 g of nitrate rich vegetables such as beetroot or spinach.

The intervention reduced BMR by 4.3% after nitrate administration compared with placebo (p<0.02). A strong negative correlation was found between the change in salivary nitrate and the change in BMR (r2=0.72; p<0.002). In addition, nitrate supplementation reduced plasma levels of malondialdehyde, indicating lower oxidative stress as a result of the intervention. Thyroid hormone status was unaffected.

The cuisines of cultures known for their longevity are usually rich in vegetables and future studies will reveal whether this life span extension is linked to the high nitrate content in this food group.

O2k-Network Lab: SE Stockholm Weitzberg E, SE Stockholm Larsen FJ

Labels: MiParea: Respiration, Exercise physiology;nutrition;life style  Pathology: Aging;senescence  Stress:Oxidative stress;RONS  Organism: Human 

Preparation: Intact organism 


Affiliations and author contributions

Dept Physiology & Pharmacology, Karolinska Institutet, Stockholm, Sweden. - Email:


  1. Larsen FJ, Weitzberg E, Lundberg JO, Ekblom B (2007) Effects of dietary nitrate on oxygen cost during exercise. Acta Physiol (Oxf) 191: 59-66.
  2. Carlström M, Persson AE, Larsson E, Hezel M, Scheffer PG, Teerlink T, Weitzberg E, Lundberg JO (2011) Dietary nitrate attenuates oxidative stress, prevents cardiac and renal injuries, and reduces blood pressure in salt-induced hypertension. Cardiovasc Res 89: 574-585.