Schleier 2020 J Cardiovasc Transl Res

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Schleier Y, Moreno-Loaiza O, López Alarcón MM, Lopes Martins EG, Braga BC, Ramos IP, Galina A, Medei EH (2020) NOD mice recapitulate the cardiac disturbances observed in type 1 diabetes. J Cardiovasc Transl Res [Epub ahead of print].

» PMID: 32468298

Schleier Y, Moreno-Loaiza O, Lopez Alarcon MM, Lopes Martins EG, Braga BC, Ramos IP, Galina A, Medei EH (2020) J Cardiovasc Transl Res

Abstract: This work aimed at testing the hypothesis that NOD/ShiLtJ mice (NOD) recapitulate the cardiac disturbances observed on type 1 diabetes (T1D). NOD mice were studied 4 weeks after the onset of hyperglycemia, and NOR/Lt mice matched as control. Cardiac function was evaluated by echocardiography and electrocardiography (ECG). Action potentials (AP) and Ca2+ transients were evaluated at whole heart level. Heart mitochondrial function was evaluated by high-resolution respirometry and H2O2 release. NOD mice presented a reduction in hearth weight. Mitochondrial oxygen fluxes and H2O2 release were similar between NOD and NOR mice. ECG revealed a QJ interval prolongation in NOD mice. Furthermore, AP duration at 30% of repolarization was increased, and it depicted slower Ca2+ transient kinetics. NOD mice presented greater number/severity of ventricular arrhythmias both in vivo and in vitro. In conclusion, NOD mice evoked cardiac electrical and calcium handling disturbances similar to the observed in T1D.

Keywords: Arrhythmias, Electrophysiology. Mitochondria. NOD mice, Type 1 diabetes Bioblast editor: Plangger M O2k-Network Lab: BR Rio de Janeiro Galina A, BR Rio de Janeiro Institute Biomedical Chemistry


Labels: MiParea: Respiration  Pathology: Diabetes 

Organism: Mouse  Tissue;cell: Heart  Preparation: Isolated mitochondria 


Coupling state: LEAK, OXPHOS  Pathway: N, NS, ROX  HRR: Oxygraph-2k 

2020-06