Schmitt 2013 Anal Biochem

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Schmitt S, Saathoff F, Meissner L, Schropp E, Lichtmannegger J, Schulz S, Eberhagen C, Borchard S, Aichler M, Adamski J, Plesnila N, Rothenfusser S, Kroemer G, Zischka H (2013) A semi-automated method for isolating functionally intact mitochondria from cultured cells and tissue biopsies. Anal Biochem 443:66-74.

» PMID: 23969012

Schmitt Sabine, Saathoff Friederike, Meissner Lilja, Schropp Eva-Maria, Lichtmannegger Josef, Schulz Sabine, Eberhagen Carola, Borchard Sabine, Aichler Michaela, Adamski Jerzy, Plesnila Nikolaus, Rothenfusser Simon, Kroemer Guido, Zischka Hans (2013) Anal Biochem

Abstract: Mitochondrial dysfunctions decisively contribute to the progression of human diseases, implying that functional tests of isolated mitochondria may furnish conclusive information for diagnosis and therapy. Classical mitochondrial isolation methods, however, lack precisely adjustable settings for cell rupture, which is the most critical step in this procedure, and this complicates subsequent analyses. Here, we present an efficient method to isolate functionally active, intact mitochondria from cultured or primary cells and minute tissue samples in a rapid, highly reproducible manner.

Bioblast editor: Gnaiger E O2k-Network Lab: DE Munich Zischka H

Cited by

  • Komlódi T, Schmitt S, Zdrazilova L, Donnelly C, Zischka H, Gnaiger E (2022) Oxygen dependence of hydrogen peroxide production in isolated mitochondria and permeabilized cells. MitoFit Preprints 2022 (in prep).

Labels: MiParea: Respiration, Instruments;methods 

Preparation: Isolated mitochondria 

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