Sjoevall 2013 Mitochondrion
|Sjövall F, Ehinger JK, Marelsson SE, Morota S, Asander Frostner E, Uchino H, Lundgren J, Arnbjörnsson E, Hansson Magnus J, Fellman V, Elmér E (2013) Mitochondrial respiration in human viable platelets - methodology and influence of gender, age and storage. Mitochondrion 13:7-14.|
Abstract: Studying whole cell preparations with intact mitochondria and respiratory complexes has a clear benefit compared to isolated or disrupted mitochondria due to the dynamic interplay between mitochondria and other cellular compartments. Platelet mitochondria have a potential to serve as a source of human viable mitochondria when studying mitochondrial physiology and pathogenic mechanisms, as well as for the diagnostics of mitochondrial diseases. The objective of the present study was to perform a detailed evaluation of platelet mitochondrial respiration using high-resolution respirometry. Further, we aimed to explore the limits of sample size and the impact of storage as well as to establish a wide range of reference data from different pediatric and adult cohorts. Our results indicate that platelet mitochondria are well suited for ex-vivo analysis with the need for minute sample amounts and excellent reproducibility and stability.
• Keywords: Diagnostics; Human; Platelets; Mitochondria; OXPHOS; High-resolution respirometry; Mitochondrial disorders
Labels: MiParea: Respiration, Instruments;methods, mt-Medicine, Patients Pathology: Aging;senescence, Neurodegenerative Stress:Ischemia-reperfusion Organism: Human Tissue;cell: Blood cells, Platelet Preparation: Intact cells, Permeabilized cells
Coupling state: LEAK, ROUTINE, OXPHOS, ET Pathway: N, ROX HRR: Oxygraph-2k
Blood cell preparation