Slater 1952 Nature

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Slater EC, Cleland KW (1952) Stabilization of oxidative phosphorylation in heart-muscle sarcosomes. Nature 170:118-9.

» PMID: 14957042

Slater EC, Cleland KW (1952) Nature

Abstract: FRESHLY prepared suspensions of the granular components of heart-muscle (sarcosomes, interstitial granules or mitochondria) are able to oxidize a number of intermediary metabolites, and to couple this oxidation with the synthesis of adenosine triphosphate from adenosine diphosphate and inorganic phosphate (oxidative phosphorylation). In agreement with the experience of other workers using preparations from different tissues, it has been found that the heart-muscle sarcosomes rapidly lose their ability to carry out oxidative phosphorylation, especially at temperatures above 0 °C. Such aged preparations oxidize succinate at practically unimpaired rate, but the accompanying phosphorylation is lost. The ability to oxidize α-ketoglutarate is lost to about the same extent as the phosphorylation associated with this oxidation, so that the P:O ratio (the number of atoms of inorganic phosphate esterified per atom of oxygen consumed) is not greatly affected. The lability of rat heart sarcosomes is indicated by the following percentages of inactivation of the α-keto-glutaric oxidase system (the enzyme complex required for the aerobic oxidation of α-ketoglutarate to succinate): 10 min standing at 15.5 °C, 52 % inactivation; 15 min at 25 °C, 94 % inactivation. Cat preparations are somewhat more stable (15 min standing at 25 °C, 71 % inactivation).

Cited by

Gnaiger 2020 BEC MitoPathways


Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2:112 pp. doi:10.26124/bec:2020-0002


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Made history, BEC 2020.2