Smenes 2018 Interact Cardiovasc Thorac Surg

From Bioblast
Publications in the MiPMap
Smenes BT, Bækkerud FH, Slagsvold KH, Hassel E, Wohlwend M, Pinho M, Høydal M, Wisløff U, Rognmo Ø, Wahba A (2018) Acute exercise is not cardioprotective and may induce apoptotic signalling in heart surgery: a randomized controlled trial. Interact Cardiovasc Thorac Surg 27:95-101.

Β» PMID: 29447379 Open Access

Smenes BT, Baekkerud FH, Slagsvold KH, Hassel E, Wohlwend M, Pinho M, Hoeydal M, Wisloeff U, Rognmo Oe, Wahba A (2018) Interact Cardiovasc Thorac Surg

Abstract: During open-heart surgery, the myocardium experiences ischaemia-reperfusion injury. A single bout of moderate, 30-min exercise induces preconditioning and protects the heart from ischaemia-reperfusion injury in rats, but this has never been investigated in humans. We aimed to investigate whether 1 bout of moderate exercise 24 h prior to surgery protects against mitochondrial and cardiac damage.

Patients scheduled for elective coronary artery bypass were eligible for this pilot study. Twenty were included and randomized to the treadmill exercise group (the EX group, n = 10) 24 h preoperatively or to standard presurgical procedures (control n = 10). Right atrial (RA) and left ventricular (LV) biopsies were collected immediately before and as long as possible after aortic cross-clamping to assess the primary outcome of mitochondrial respiration by respirometry, in addition to reactive oxygen species production by fluorometry and apoptotic transcripts. Cardiac troponin T and creatine kinase myocardial brain were measured in plasma at arrival, before surgery and 6 and 24 h postoperatively.

Mitochondrial respiration was lower in the EX group after surgery in the LV (Complex I -22%, P < 0.05 and maximal -23%, P < 0.05) and the right atrium (Complex I -25%, P < 0.05). Transcript level of the apoptosis-related marker caspase 3 was increased 1.5-fold in the LV prior to surgery in the EX group when compared with the control group, P < 0.05. Cardiac troponin T was 45% higher in the EX group than in the control group 6 h postoperatively (P = 0.03), although not significant when corrected for aortic cross-clamping time.

Results indicate that exercise did not precondition the heart against surgery-related damage. Exercise may render the myocardium and mitochondria more vulnerable to perioperative damage.

Β© The Author(s) 2018. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved. β€’ Keywords: Caspase-3, Ischaemia–reperfusion, Mitochondria, Preconditioning, Troponin, Amplex Red in muscle fibers β€’ Bioblast editor: Kandolf G, Smenes BT β€’ O2k-Network Lab: NO Trondheim Rognmo O

Labels: MiParea: Respiration, Exercise physiology;nutrition;life style, Patients  Pathology: Aging;senescence, Cardiovascular  Stress:Ischemia-reperfusion  Organism: Human  Tissue;cell: Heart  Preparation: Permeabilized tissue 

Coupling state: LEAK, OXPHOS, ET  Pathway: F, N, S, CIV, NS, ROX  HRR: Oxygraph-2k, O2k-Fluorometer 

2018-03, AmR 

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