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Smoothing
Description
Various methods of smoothing can be applied to improve the signal-to-noise ratio. For instance, data points recorded over time [s] or over a range of wavelengths [nm] can be smoothed by averaging n data points per interval. Then the average of the n points per smoothing interval can be taken for each successively recorded data point across the time range or range of the spectrum to give a n-point moving average smoothing. This method decreases the noise of the signal, but clearly reduces the time or wavelength resolution. More advanced methods of smoothing are applied to retain a higher time resolution or wavelength resolution.
Reference: Press 1990 Computers in Physics
MitoPedia concepts:
MitoFit Quality Control System
MitoPedia methods:
Respirometry,
Fluorometry,
Spectrophotometry
MitoPedia O2k and high-resolution respirometry:
DatLab
Communicated by Harrison DK, 2011-11-25
DatLab oxygen flux: performance and data analysis
- Recommendation: Set "Slope smoothing" to 20 for performing and analyzing experiments with biological sample (strongly recommended in the case of uncoupler titrations). A slope smoothing of 20 increases the time or wavelength resolution, but increases the noise of the signal.
- The following DatLab traces illustrate an example of "Slope smoothing" using 20 versus 40 data points:
DatLab Amp slope: performance and data analysis
- Recommendation: Set "Slope smoothing" of the Amp slope to 20 for performing and analyzing experiments with biological sample (strongly recommended in the case of uncoupler titrations). A slope smoothing of 20 increases the time or wavelength resolution, but increases the noise of the signal.
- The following DatLab traces illustrate an example of "Slope smoothing" using 20 versus 40 data points:
- Slope smoothing: 20
- Slope smoothing: 40