Soendergaard 2016 Eur J Sport Sci
SΓΈndergΓ₯rd SD, Dela F, Helge JW, Larsen S (2016) Actovegin, a non-prohibited drug increases oxidative capacity in human skeletal muscle. Eur J Sport Sci 16:801-7. |
Soendergaard SD, Dela F, Helge JW, Larsen S (2016) Eur J Sport Sci
Abstract: Actovegin, a deproteinized haemodialysate of calf blood, is suggested to have ergogenic properties, but this potential effect has never been investigated in human skeletal muscle. To investigate this purported ergogenic effect, we measured the mitochondrial respiratory capacity in permeabilized human skeletal muscle fibres acutely exposed to Actovegin in a low and in a high dose. We found that Actovegin, in the presence of complex I-linked substrates increased the oxidative phosphorylation (OXPHOS) capacity significantly in a concentration-dependent manner (19βΒ±β3, 31βΒ±β4 and 45βΒ±β4β pmol/mg/s). Maximal OXPHOS capacity with complex I and II-linked substrate was increased when the fibres were exposed to the high dose of Actovegin (62βΒ±β6 and 77βΒ±β6β pmol/mg/s) (pβ<β.05). The respiratory capacity of the electron transfer-pathway as well as Vmax and Km were also increased in a concentration-dependent manner after Actovegin exposure (70βΒ±β6, 79βΒ±β6 and 88βΒ±β7β pmol/mg/s; 13βΒ±β2, 25βΒ±β3 and 37βΒ±β4β pmol/mg/s; 0.08βΒ±β0.02, 0.21βΒ±β0.03 and 0.36βΒ±β0.03β mM, respectively) (pβ<β.05). In summary, we report for the first time that Actovegin has a marked effect on mitochondrial oxidative function in human skeletal muscle. Mitochondrial adaptations like this are also seen after a training program in human subjects. Whether this improvement translates into an ergogenic effect in athletes and thus reiterates the need to include Actovegin on the World Anti-Doping Agency's active list remains to be investigated. β’ Keywords: Actovegin, Human skeletal muscle, Mitochondrial oxidative capacity, Non-prohibited drug
β’ O2k-Network Lab: DK Copenhagen Dela F, DK Copenhagen Larsen S
Labels: MiParea: Respiration, Exercise physiology;nutrition;life style, Pharmacology;toxicology
Organism: Human
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue
Regulation: ADP Coupling state: LEAK, OXPHOS, ET Pathway: N, NS HRR: Oxygraph-2k
2016-02