Sonkar 2013 Abstract MiP2013

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Sonkar VK, Dash D, Kulkarn PP (2013) Platelets, amyloid beta and mitochondrial respiration. Mitochondr Physiol Network 18.08.
Link:
Vijay K Sonkar
MiP2013, Book of Abstracts Open Access

Sonkar VK, Dash D, Kulkarn PP (2013)

Event: MiPNet18.08_MiP2013

Platelets or thrombocytes are the key players in hemostasis and contain >90% of the circulating amyloid precursor protein, which upon proteolytic cleavage by β-secretase BACE1 (β-site APP cleaving enzyme 1) and the presenilin-containing γ-secretase complex produces amyloid beta peptide (Aβ). Aβ, a major mediator of neuronal death, is also cytotoxic to cerebral endothelial cells, vascular smooth muscle cells and can accumulate in the cerebral blood vessels leading to cerebral amyloid angiopathy. In this report we have evaluated the effect of Aβ active fragment containing amino acid sequence 25-35 (Aβ25-35) on platelet activity and mitochondrial respiration. Exogenous Aβ25-35 was found to induce platelet aggregation, PAC-1binding and P-selectin exposure in washed human platelets, which was comparable to that induced by other physiological agonists. Aβ25-35 also induced ATP release indicative of secretion from platelet dense granules. High-resolution respirometry (HRR) showed that Aβ25-35 elicited sharp rise in mitochondrial respiration in intact platelets. Thus, Aβ induced platelet activation that was associated with an increase in oxygen consumption rate.

Keywords: Platelets, Amyloid beta (Aβ25-35)

O2k-Network Lab: IN Varanasi Dash D


Labels: MiParea: Respiration, mt-Medicine  Pathology: Alzheimer's, Neurodegenerative 

Organism: Human  Tissue;cell: Blood cells  Preparation: Intact cells 

Regulation: ATP  Coupling state: ROUTINE 

HRR: Oxygraph-2k 

MiP2013 

Affiliations and author contributions

Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.

Email: sonkarvijay@gmail.com

  • Corresponding author: ddass@satyam.net.in

References

  1. Smith CCT (1997) Stimulated release of the b-amyloid protein of Alzheimer’s disease by normal human platelets. Neurosci Lett 235: 157–159.
  2. Hsu MJ, Hsu CY, Chen BC, Chen MC, Ou G, Lin CH, (2007) Apoptosis signal regulating kinase 1 in amyloid beta peptide-induced cerebral endothelial cell apoptosis. J Neurosci 27: 5719–5729.
  3. Shen MY, Hsiao G, Fong TH, Chen HM, Chou DS, Lin CH, Sheu JR, Hsu CY (2008) Amyloid beta peptide-activated signal pathways in human platelets. Euro J Pharmacol 588: 259-266