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Adams 2010 Biochim Biophys Acta
Has abstract Our laboratory has previously demonstrated
Our laboratory has previously demonstrated the presence of constitutively expressed mitochondrial uncoupling protein 1 in mouse thymocytes. In our endeavours to understand the role of mitochondrial uncoupling protein 1 in thymocyte function, we compared cell profiles in thymus and spleen of wild-type with those of UCP 1 knock-out mice, which in turn led to comparative investigations of apoptotic potential in thymocytes from these mice. We demonstrate that spleen cell numbers were reduced ∼3-fold in UCP 1 knock-out mice compared to wild-type mice. We record a halving of CD8 single positive cell numbers in thymus with a significant incremental increase in CD4/CD8 double positives cell numbers in the thymus of UCP 1 knock-out mice compared to wild-type mice. These data are mirrored by an approximate halving of CD8 single positive cell numbers and a doubling of CD4/CD8 double positive cell numbers in the spleen of UCP 1 knock-out mice compared to wild-type mice. These differences are most probably explained by our observations of decreased apoptotic potential and higher ATP levels in thymocytes of UCP 1 knock-out mice when compared to wild-type controls. We conclude that constitutively expressed UCP 1 is a factor in determining T-cell population selection in mice.
ining T-cell population selection in mice.  +
Has info [http://www.ncbi.nlm.nih.gov/pubmed/20417612 PMID: 20417612 Open Access]  +
Has publicationkeywords Mitochondria  + , Uncoupling protein  + , Thymus  + , Spleen  +
Has title Adams AE, Kelly OM, Porter RK (2010) Absence of mitochondrial uncoupling protein 1 affects apoptosis in thymocytes, thymocyte/T-cell profile and peripheral T-cell number. Biochim Biophys Acta 1797:807-16.  +
Instrument and method Oxygraph-2k  +
Mammal and model Mouse  +
Preparation Intact cells  +
Tissue and cell Islet cell;pancreas;thymus  +
Was published by MiPNetLab IE Dublin Porter RK +
Was published in journal Biochim Biophys Acta +
Was published in year 2010  +
Was written by Adams AE + , Kelly OM + , Porter RK +
Categories Publications
Modification date
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09:22:44, 26 May 2015  +
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