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Ali 2015 Abstract MiP2015
Additional label MiP2015  +
Coupling states LEAK  + , OXPHOS  + , ET  +
Event C1  + , Oral  +
Has abstract Gender-specific differences in mitochondri
Gender-specific differences in mitochondrial function and free radical homeostasis are consistently reported in the context of aging and associated deficits. However, little is known about the gender-related roles of these parameters in the pathogenesis of neurological and cardiovascular disorders that occur early in life. Aim: To test the hypothesis that gender disparity in mitochondria function and ROS homeostasis starts early in life and hence can be implicated in sexual dimorphism in some cardiac as well as neurological disorders. Approach: We investigated heart and brain mitochondrial respiratory function in young (2-4 months) male and female wild-type C57BL6J mice, by high-resolution respirometry. Parallel productions of ROS by respiring mitochondria or active NADPH oxidases (NOXs) were also assessed using high-resolution oxymetry, fluorescence assays, and electron paramagnetic resonance (EPR) spin trapping techniques. Results: Although mitochondrial respiratory activity in the heart did not significantly vary between genders, female brains exhibited enhanced activity during state 3, state 4, and maximally uncoupled respiration. This was associated with lower rates of hydrogen peroxide production in female cardiac and brain tissues. Furthermore, no gender differences have been detected in Nox2 and Nox4 proteins or activities in brain homogenate or freshly isolated synaptosomes. However, a strong trend of increased EPR-detected NOX-superoxide in male synaptosomes hinted at gender-specific discrepancy in antioxidant enzymes. Indeed, we found that superoxide dismutase (SOD) activity was higher in female brains using two independent approaches. Conclusion: Taken together, our results indicate that gender differences in mitochondrial bioenergetics and ROS production occur at young age, and that differences in superoxide dismutation capacity may be primarily responsible for gender differences in ROS homeostasis. These findings may eventually assist in the understanding of sexual dimorphism in some disorders that occur early in life.
n some disorders that occur early in life.  +
Has title Mitochondrial function and ROS homeostasis in young mouse heart and brain: the sex factor!  +
Instrument and method Oxygraph-2k  + , O2k-Fluorometer  +
Mammal and model Mouse  +
MiP area Respiration  + , Gender  +
Preparation Homogenate  +
Tissue and cell Heart  + , Nervous system  +
Was published by MiPNetLab EG Cairo Ali SS +
Was submitted in year 2015  +
Was submitted to event MiP2015 +
Was written by Khalifa AM + , Abdel-Rahman E + , Mahmoud AM + , Ali MH + , Ali SS +
Categories Abstracts
Modification date
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08:10:28, 9 April 2018  +
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