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Ali 2015 Abstract MiPschool Cape Town 2015
Coupling states LEAK  + , OXPHOS  + , ET  +
Has abstract Mitochondrial dysfunction and oxidative st
Mitochondrial dysfunction and oxidative stress are proposed as key elements in the pathogenesis of aging, as well as neurological and cardiovascular disorders that occur early in life. Sex differences in free radical homeostasis upon aging have been extensively studied. However, little is known about gender differences in mitochondrial function and dynamics of ROS sources that may develop in young ages and hence contribute to sexual dimorphism in some disorders that occur early in life. We investigated heart and brain mitochondrial respiratory function and ROS production in young (2-5 months) male and female wild-type C57BL6 mice using the Oroboros Oxygraph-2k. Mitochondrial respiratory activity in heart did not significantly differ between genders. However, female brains had an enhanced mitochondrial respiratory activity during state 3, state 4, and maximally uncoupled respiration as compared to male brains. This respiratory activity observed in mitochondria from female heart and brain was associated with lower rates of hydrogen peroxide production in cardiac and neuronal female mitochondria as compared to male. By using two different approaches, we also found that superoxide dismutase (Sod) activity was higher in female brains, suggesting that enhanced antioxidant defenses in female brains contribute to gender differences in ROS levels. Neither protein expression of NADPH oxidases (Nox2 & Nox4) in brain homogenate or synaptosomes, nor the Oroboros determined activity of these enzymes changed between genders. Paradoxically, when Nox-superoxide was assessed in synaptosomes using spin trapping electron paramagnetic resonance spectroscopy, males exhibited higher activity. We conclude that gender differences in mitochondrial function and ROS production occur in young age, and that differences in antioxidant buffering capacity between genders may be primarily responsible for gender differences in brain ROS homeostasis.
nder differences in brain ROS homeostasis.  +
Has title Early gender-related dimorphism in heart and brain mitochondria function and ROS dynamics.  +
Instrument and method Oxygraph-2k  +
Mammal and model Mouse  +
MiP area Respiration  + , Gender  +
Tissue and cell Heart  + , Nervous system  +
Was published by MiPNetLab EG Cairo Ali SS +
Was submitted in year 2015  +
Was submitted to event MiPschool Cape Town 2015 +
Was written by Ali SS + , Abdel-Rahman EA + , Khalifa AM + , Mahmoud AM + , Ali MH +
Categories Abstracts
Modification date
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11:16:17, 23 January 2019  +
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