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Anderson 2017 Cell Metab
Additional label 2017-07  +
Coupling states OXPHOS  + , LEAK  +
Diseases Aging;senescence  + , Diabetes  +
Has abstract Sirtuins are NAD<sup>+</sup>-d
Sirtuins are NAD<sup>+</sup>-dependent protein deacylases that regulate several aspects of metabolism and aging. In contrast to the other mammalian sirtuins, the primary enzymatic activity of mitochondrial sirtuin 4 (SIRT4) and its overall role in metabolic control have remained enigmatic. Using a combination of phylogenetics, structural biology, and enzymology, we show that SIRT4 removes three acyl moieties from lysine residues: methylglutaryl (MG)-, hydroxymethylglutaryl (HMG)-, and 3-methylglutaconyl (MGc)-lysine. The metabolites leading to these post-translational modifications are intermediates in leucine oxidation, and we show a primary role for SIRT4 in controlling this pathway in mice. Furthermore, we find that dysregulated leucine metabolism in SIRT4KO mice leads to elevated basal and stimulated insulin secretion, which progressively develops into glucose intolerance and insulin resistance. These findings identify a robust enzymatic activity for SIRT4, uncover a mechanism controlling branched-chain amino acid flux, and position SIRT4 as a crucial player maintaining insulin secretion and glucose homeostasis during aging. Copyright © 2017 Elsevier Inc. All rights reserved.
© 2017 Elsevier Inc. All rights reserved.  +
Has editor [[Kandolf G]]  +
Has info [ PMID: 28380376]  +
Has publicationkeywords Branched-chain amino acids  + , Deacylase  + , Insulin secretion  + , Leucine  + , Mitochondria  + , Sirtuin 4  +
Has title Anderson KA, Huynh FK, Fisher-Wellman K, S
Anderson KA, Huynh FK, Fisher-Wellman K, Stuart JD, Peterson BS, Douros JD, Wagner GR, Thompson JW, Madsen AS, Green MF, Sivley RM, Ilkayeva OR, Stevens RD, Backos DS, Capra JA, Olsen CA, Campbell JE, Muoio DM, Grimsrud PA, Hirschey MD (2017) SIRT4 is a lysine deacylase that controls leucine metabolism and insulin secretion. Cell Metab 25:838-55.
d insulin secretion. Cell Metab 25:838-55.  +
Instrument and method Oxygraph-2k  +
Mammal and model Mouse  +
MiP area Respiration  + , Genetic knockout;overexpression  +
Pathways F  + , N  + , NS  + , Other combinations  +
Preparation Isolated mitochondria  +
Tissue and cell Heart  + , Liver  +
Was published in journal Cell Metab +
Was published in year 2017  +
Was written by Anderson KA + , Huynh FK + , Fisher-Wellman K + , Stuart JD + , Peterson BS + , Douros JD + , Wagner GR + , Thompson JW + , Madsen AS + , Green MF + , Sivley RM + , Ilkayeva OR + , Stevens RD + , Backos DS + , Capra JA + , Olsen CA + , Campbell JE + , Muoio DM + , Grimsrud PA + , Hirschey MD +
Categories Publications
Modification date
"Modification date" is a predefined property that corresponds to the date of the last modification of a subject and is provided by Semantic MediaWiki.
09:54:45, 31 July 2017  +
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