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Astin 2013 Sci Rep
Coupling states LEAK  + , OXPHOS  +
Has abstract The circulating, endocrine renin-angiotens
The circulating, endocrine renin-angiotensin system (RAS) is important to circulatory homeostasis, while ubiquitous tissue and cellular RAS play diverse roles, including metabolic regulation. Indeed, inhibition of RAS is associated with improved cellular oxidative capacity. Recently it has been suggested that an intra-mitochondrial RAS directly impacts on metabolism. Here we sought to rigorously explore this hypothesis. Radiolabelled ligand-binding and unbiased proteomic approaches were applied to purified mitochondrial sub-fractions from rat liver, and the impact of AngII on mitochondrial function assessed. Whilst high-affinity AngII binding sites were found in the mitochondria-associated membrane (MAM) fraction, no RAS components could be detected in purified mitochondria. Moreover, AngII had no effect on the function of isolated mitochondria at physiologically relevant concentrations. We thus found no evidence of endogenous mitochondrial AngII production, and conclude that the effects of AngII on cellular energy metabolism are not mediated through its direct binding to mitochondrial targets.
s direct binding to mitochondrial targets.  +
Has info [ PMID:23959064 Open Access]  +
Has title Astin R, Bentham R, Djafarzadeh S, Horscro
Astin R, Bentham R, Djafarzadeh S, Horscroft JA, Kuc RE, Leung PS, Skipworth JR, Vicencio JM, Davenport AP, Murray AJ, Takala J, Jakob SM, Montgomery H, Szabadkai G (2013) No evidence for a local renin-angiotensin system in liver mitochondria. Sci Rep 3:2467.
tem in liver mitochondria. Sci Rep 3:2467.  +
Instrument and method Oxygraph-2k  +
Mammal and model Rat  +
MiP area Respiration  +
Pathways N  + , S  + , CIV  +
Preparation Isolated mitochondria  +
Tissue and cell Liver  +
Was published by MiPNetLab UK Cambridge Murray AJ + , CH Bern Djafarzadeh S +
Was published in journal Sci Rep +
Was published in year 2013  +
Was written by Astin R + , Bentham R + , Djafarzadeh Siamak + , Horscroft JA + , Kuc RE + , Leung PS + , Skipworth JR + , Vicencio JM + , Davenport AP + , Murray AJ + , Takala J + , Jakob SM + , Montgomery H + , Szabadkai G +
Categories Publications
Modification date
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11:22:37, 23 January 2019  +
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