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Bai 2016 PLOS ONE
Additional label 2016-07  +
Coupling states LEAK  + , OXPHOS  + , ET  +
Has abstract Oxygen consumption by isolated mitochondri
Oxygen consumption by isolated mitochondria is generally measured during state 4 respiration (no ATP production) or state 3 (maximal ATP production at high ADP availability). However, mitochondria ''in vivo'' do not function at either extreme. Here we used ADP recycling methodology to assess muscle mitochondrial function over intermediate clamped ADP concentrations. In so doing, we uncovered a previously unrecognized biphasic respiratory pattern wherein O<sub>2</sub> flux on the complex II substrate, succinate, initially increased and peaked over low clamped ADP concentrations then decreased markedly at higher clamped concentrations. Mechanistic studies revealed no evidence that the observed changes in O<sub>2</sub> flux were due to altered opening or function of the mitochondrial permeability transition pore or to changes in reactive oxygen. Based on metabolite and functional metabolic data, we propose a multifactorial mechanism that consists of coordinate changes that follow from reduced membrane potential (as the ADP concentration in increased). These changes include altered directional electron flow, altered NADH/NAD<sup>+</sup> redox cycling, metabolite exit, and OAA inhibition of succinate dehydrogenase. In summary, we report a previously unrecognized pattern for complex II energized O<sub>2</sub> flux. Moreover, our findings suggest that the ADP recycling approach might be more widely adapted for mitochondrial studies.
widely adapted for mitochondrial studies.  +
Has info [ PMID: 27153112 Open Access]  +
Has publicationkeywords Amplex Red  +
Has title Bai F, Fink BD, Yu L, Sivitz WI (2016) Voltage-dependent regulation of Complex II energized mitochondrial oxygen flux. PLOS ONE 11:e0154982.  +
Instrument and method Oxygraph-2k  + , TPP  +
Mammal and model Mouse  +
MiP area Respiration  + , Instruments;methods  +
Pathways N  + , S  + , CIV  + , NS  +
Preparation Isolated mitochondria  +
Respiration and regulation ADP  +
Stress Oxidative stress;RONS  +
Tissue and cell Heart  + , Skeletal muscle  + , Nervous system  + , Liver  +
Was published by MiPNetLab US IA Iowa City Sivitz WI +
Was published in journal PLOS ONE +
Was published in year 2016  +
Was written by Bai F + , Fink BD + , Yu L + , Sivitz WI +
Categories Publications
Modification date
"Modification date" is a predefined property that corresponds to the date of the last modification of a subject and is provided by Semantic MediaWiki.
14:52:03, 9 November 2017  +
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