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Beard 2005 PLOS Comput Biol
Coupling states OXPHOS  +
Has abstract A computational model for the mitochondria
A computational model for the mitochondrial respiratory chain that appropriately balances mass, charge, and free energy transduction is introduced and analyzed based on a previously published set of data measured on isolated cardiac mitochondria. The basic components included in the model are the reactions at Complexes I, III, and IV of the electron transport system, ATP synthesis at F1FO ATPase, substrate transporters including adenine nucleotide translocase and the phosphate-hydrogen co-transporter, and cation fluxes across the inner membrane including fluxes through the K<sup>+</sup>/H<sup>+</sup> antiporter and passive H<sup>+</sup> and K<sup>+</sup> permeation. Estimation of 16 adjustable parameter values is based on fitting model simulations to nine independent data curves. The identified model is further validated by comparison to additional datasets measured from mitochondria isolated from rat heart and liver and observed at low oxygen concentration. To obtain reasonable fits to the available data, it is necessary to incorporate inorganic-phosphate-dependent activation of the dehydrogenase activity and the electron transport system. Specifically, it is shown that a model incorporating phosphate-dependent activation of Complex III is able to reasonably reproduce the observed data. The resulting validated and verified model provides a foundation for building larger and more complex systems models and investigating complex physiological and pathophysiological interactions in cardiac energetics.
ogical interactions in cardiac energetics.  +
Has editor [[Gnaiger E]]  +
Has info [ PMID: 16163394 Open Access]  +
Has title Beard DA (2005) A biophysical model of the mitochondrial respiratory system and oxidative phosphorylation. PLoS Comput Biol 1(4):e36.  +
MiP area Respiration  +
Respiration and regulation ADP  + , Calcium  + , Flux control  + , Ion;substrate transport  + , mt-Membrane potential  + , Oxygen kinetics  + , Redox state  +
Was published by MiPNetLab US MI Ann Arbor Beard DA +
Was published in journal PLoS Comput Biol +
Was published in year 2005  +
Was written by Beard DA +
Categories Publications
Modification date
"Modification date" is a predefined property that corresponds to the date of the last modification of a subject and is provided by Semantic MediaWiki.
09:03:38, 31 October 2017  +
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