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Dejean 2002 Biochem Biophys Res Comm
Coupling states OXPHOS  +
Has abstract We investigated the effects of genetic and
We investigated the effects of genetic and physiological modulations of the cAMP–protein kinase A pathway on mitochondrial biogenesis of yeast cells grown on lactate. Yeast mutants with over-activated Ras/adenylate cyclase pathway (i.e., ''Ras2<sup>val19</sup>, ira1Δira2Δ'') or with a constitutive downstream activation of protein kinases A (i.e., bcyΔ) showed an increase in the mitochondrial enzyme content. In contrast, loss of Ras activity (i.e., ''Ras2'' mutant) resulted in a slight decrease. The treatment by cAMP of a responsive mutant increased the oxidative phosphorylation capacity of cells and increased the transcript level of nuclear genes encoding for mitochondrial proteins. In contrast, the transcript level of mitochondrial DNA genes was unchanged. It is concluded that the Ras/cAMP/protein kinase A pathway is part of the regulatory circuit controlling biogenesis of the oxidative phosphorylation complexes in yeast cells.
phosphorylation complexes in yeast cells.  +
Has info [ PMID: 12054668]  +
Has publicationkeywords cAMP  + , ''Saccharomyces cerevisiae''  + , Ras mutants  + , Mitochondria  + , Cytochromes  + , Respiration  +
Has title Dejean L, Beauvoit B, Bunoust O, Guerin B, Rigoulet M (2002) Activation of Ras cascade increases the mitochondrial enzyme content of respiratory competent yeast. Biochem Biophys Res Comm 293:1383-8.  +
Instrument and method Oxygraph-2k  +
Mammal and model Saccharomyces cerevisiae  + , Fungi  +
MiP area Respiration  + , Genetic knockout;overexpression  +
Preparation Intact cells  +
Was published by MiPNetLab FR Pessac Diolez P + , FR Bordeaux Devin A +
Was published in journal Biochem Biophys Res Commun +
Was published in year 2002  +
Was written by Dejean L + , Beauvoit B + , Bunoust O + , Guerin B + , Rigoulet M +
Categories Publications
Modification date
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10:43:28, 16 February 2018  +
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