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Ferko 2014 Physiol Res
Coupling states LEAK  + , OXPHOS  +
Has abstract Remote ischemic preconditioning (RIP)-indu
Remote ischemic preconditioning (RIP)-induced protection of myocardial energetics was well documented on the level of tissue, but data concerning the involvement of mitochondria were missing. We aimed at the identification of changes in membrane properties and respiratory functions induced in rat heart mitochondria by RIP. Experiments were performed on 46 male Wistar rats divided into control and RIP-treated groups of 21 animals each. Blood flow in the occluded area was recorded by MRI angiography in four animals. RIP protocol comprised of three successive 5-min occlusions each followed by 5-min reperfusions of descending branches of the right hind limb femoral artery. The efficacy of RIP was evaluated as the extent of RIP-induced protection against damage to the functions of mitochondria isolated by differential centrifugation after 30-min global ischemia followed by 40-min reperfusion of the hearts in Langendorff mode. Assessments: Mitochondrial membrane fluidity with a fluorescent probe DPH, CoQ(9) and CoQ(10) with HPLC, mitochondrial respiration with the Oxygraph-2k (Oroboros). Results revealed that RIP was affecting the mitochondria. The immediate protection conferred by RIP involves beneficial and prognostically significant effects: a total elimination of ischemia/reperfusion-induced depression of mitochondrial membrane fluidity and a trend for better preservation of mitochondrial state 3 respiration.
tion of mitochondrial state 3 respiration.  +
Has info [ PMID:25669678]  +
Has title Ferko M, Kancirová I, Jašová M, Čarnická S
Ferko M, Kancirová I, Jašová M, Čarnická S, Muráriková M, Waczulíková I, Sumbalová Z, Kucharská J, Uličná O, Ravingerová T, Ziegelhöffer A (2014) Remote ischemic preconditioning of the heart: protective responses in functional and biophysical properties of cardiac mitochondria. Physiol Res 63:469-78.
rdiac mitochondria. Physiol Res 63:469-78.  +
Instrument and method Oxygraph-2k  +
Mammal and model Rat  +
MiP area Respiration  +
Pathways F  + , N  +
Preparation Isolated mitochondria  +
Stress Ischemia-reperfusion  +
Tissue and cell Heart  +
Was published by MiPNetLab AT Innsbruck Oroboros + , SK Bratislava Sumbalova Z +
Was published in journal Physiol Res +
Was published in year 2014  +
Was written by Ferko M + , Kancirova I + , Jasova M + , Carnicka S + , Murarikova M + , Waczulíkova I + , Sumbalova Z + , Kucharska J + , Ulicna O + , Ravingerova T + , Ziegelhoeffer A +
Categories Publications
Modification date
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13:58:50, 12 October 2017  +
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