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Garcia-Roves 2019 MiPschool Coimbra
Coupling states LEAK  + , OXPHOS  + , ET  +
Has abstract [[Image:MITOEAGLE-logo.jpg|left|100px|link
[[Image:MITOEAGLE-logo.jpg|left|100px|link=http://www.mitoeagle.org/index.php/MitoEAGLE|COST Action MitoEAGLE]] As WG2 leader in the MitoEAGLE COST Action my main role is to stimulate, engage and coordinate scientists in the different tasks to achieve our proposed deliverables. In this specific WG we should deliver: ::::#A mitochondrial database on muscle tissue from humans and model organisms. ::::#A set of guidelines for future studies on mitochondrial bioenergetics in muscle tissue. ::::#Draft of review manuscripts on these topics. In relation to MitoEAGLE data repository in muscle tissues, during my presentation I will summarize our progress in the generation of reference values for mitochondrial respirometry in permeabilized skeletal muscle sample preparations. The goal is that new researchers in the field follow a reference protocol and check if their values are in an acceptable range. This approach could serve to test researchers’ technical skills and therefore determine if they are proficient enough to perform their own experiments with confidence. We initially ran a pilot study including 7 European groups. The results obtained show, as we expected, low variability among laboratories. After some adjustments and identifying some relevant steps that need to be carefully taken into consideration, the experimental procedure and results were judged satisfactory. Therefore, during Spring 2018 a new invitation was broadly open to laboratories worldwide. The final aim is to collect all the data and write a manuscript by 2020. All research groups involved obtained respirometry reference values in permeabilized soleus fibers from males (N=4) and females (N=4) C57BL/6J mice aged 14-16 weeks. The substrate-uncoupler-inhibitor titration (SUIT) protocol used in the study is represented in Figure 1 [1]. The presentation will give detailed information on the experimental procedures followed to fulfill our objective. Finally, we will present and discuss our preliminary data, including results from the second phase where ten additional international laboratories have performed the experiments. This unique international study has the ambition to significantly contribute to major aims and concerns in the scientific community, as stablished more precisely in the MitoEAGLE memorandum of understanding: :Data sharing :#Intensify the dissemination of updated knowledge and know-how among the partners. :#Mapping mitochondrial physiology and pathology to develop a mitochondrial database. :Reproducibility crisis :#MitoEAGLE recommendations: our goal is to increase the value and reduce the noise in mitochondrial research. :#Optimally harmonize protocols across research groups.
armonize protocols across research groups.  +
Has editor [[Plangger M]]  +
Has title [[Image:Garcia-RovesP.jpg|left|90px|Pablo Garcia-Roves]] Generating reference values on mitochondrial respiration in permeabilized muscle fibers.  +
Instrument and method Oxygraph-2k  + , O2k-Protocol  +
Mammal and model Mouse  +
MiP area Respiration  + , Instruments;methods  +
Pathways N  + , S  + , CIV  + , NS  + , ROX  +
Preparation Permeabilized tissue  +
Tissue and cell Skeletal muscle  +
Was submitted in year 2019  +
Was submitted to event MiPschool Coimbra 2019 +
Was written by Garcia-Roves PM + , Dahdah N + , Gama Perez P + , Doerrier C + , Gnaiger E + , Lemieux H + , Holody C + , Carpenter RG + , Tepp K + , Puurand M + , Kaambre T + , Dubouchaud H + , Chabi B + , Cortade F + , Ost M + , Pesta D + , Calabria E + , Casado Pinna M + , Fernandez-Ortiz M + , Acuna-Castroviejo D + , Villena JA + , Grefte S + , Keijer J + , Sowton AP + , Murray AJ + , Campbell MD + , Marcinek DJ + , Nollet E + , Wuest RC + , Dayanidhi S + , O'Brien KA +
Categories Abstracts
Modification date
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09:07:06, 3 July 2019  +
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