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Krako 2013 J Alzheimers Dis
Coupling states LEAK  + , OXPHOS  +
Diseases Alzheimer's  +
Enzyme TCA cycle and matrix dehydrogenases  +
Has abstract The 7WD4 and 7PA2 cell lines, widely used
The 7WD4 and 7PA2 cell lines, widely used as cellular models for Alzheimer's disease (AD), have been used to investigate the effects of amyloid-β protein precursor overexpression and amyloid-β (Aβ) oligomer accumulation on mitochondrial function. Under standard culture conditions, both cell lines, compared to Chinese hamster ovary (CHO) control cells, displayed an ~5% decrease of O2 respiration as sustained by endogenous substrates. Functional impairment of the respiratory chain was found distributed among the protein complexes, though more evident at the level of complex I and complex IV. Measurements of ATP showed that its synthesis by oxidative phosphorylation is decreased in 7WD4 and 7PA2 cells by ~25%, this loss being partly compensated by glycolysis (Warburg effect). Compensation proved to be more efficient in 7WD4 than in 7PA2 cells, the latter cell line displaying the highest reactive oxygen species production. The strongest deficit was observed in mitochondrial membrane potential that is almost 40% and 60% lower in 7WD4 and 7PA2 cells, respectively, in comparison to CHO controls. All functional parameters point to a severe bioenergetic impairment of the AD cells, with the extent of mitochondrial dysfunction being correlated to the accumulation of Aβ peptides and oligomers.
accumulation of Aβ peptides and oligomers.  +
Has info [http://www.ncbi.nlm.nih.gov/pubmed/23948918 PMID: 23948918]  +
Has publicationkeywords Alzheimer's disease; Amyloid-β oligomers; Bioenergetics; Mitochondrial dysfunction  +
Has title Krako N, Magnifico MC, Arese M, Meli G, Forte E, Lecci A, Manca A, Giuffrè A, Mastronicola D, Sarti P, Cattaneo A (2013) Characterization of mitochondrial dysfunction in the 7PA2 cell model of Alzheimer’s disease. J Alzheimers Dis 37:747-58.  +
Instrument and method Oxygraph-2k  +
MiP area Respiration  + , Comparative MiP;environmental MiP  +
Pathways N  + , S  + , CIV  +
Preparation Permeabilized cells  +
Respiration and regulation mt-Membrane potential  +
Tissue and cell CHO  +
Was published by MiPNetLab IT Roma Sarti P +
Was published in journal J Alzheimers Dis +
Was published in year 2013  +
Was written by Krako Jakovljevic N + , Magnifico MC + , Arese M + , Meli G + , Forte E + , Lecci A + , Manca A + , Giuffre A + , Mastronicola D + , Sarti P + , Cattaneo A +
Categories Publications
Modification date
"Modification date" is a predefined property that corresponds to the date of the last modification of a subject and is provided by Semantic MediaWiki.
08:28:48, 9 November 2016  +
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