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Lemieux 2017 Sci Rep
Additional label 1PM;2D;3G;4S;5U;6Rot-  + , 1GM;2D;3P;4S;5U;6Rot-  + , MitoFitPublication  + , 2017-06  + , SUIT-008  + , SUIT-014  + , MitoEAGLEPublication  + , SUIT-008 O2 pce D025  +
Coupling states LEAK  + , OXPHOS  + , ET  +
Enzyme Marker enzyme  +
Has abstract Fuel substrate supply and oxidative phosph
Fuel substrate supply and oxidative phosphorylation are key determinants of muscle performance. Numerous studies of mammalian mitochondria are carried out (i) with substrate supply that limits electron flow, and (ii) far below physiological temperature. To analyze potentially implicated biases, we studied mitochondrial respiratory control in permeabilized mouse myocardial fibers using high-resolution respirometry. The capacity of oxidative phosphorylation at 37 °C was nearly two-fold higher when fueled by physiological substrate combinations reconstituting tricarboxylic acid cycle function, compared with electron flow measured separately through NADH to Complex I or succinate to Complex II. The relative contribution of the NADH pathway to physiological respiratory capacity increased with a decrease in temperature from 37 to 25 ºC. The apparent excess capacity of cytochrome c oxidase above physiological pathway capacity increased sharply under hypothermia due to limitation by NADH-linked dehydrogenases. This mechanism of mitochondrial respiratory control in the hypothermic mammalian heart is comparable to the pattern in ectotherm species, pointing towards NADH-linked mt-matrix dehydrogenases and the phosphorylation system rather than electron transfer complexes as the primary drivers of thermal sensitivity at low temperature. Delineating the link between stress and remodeling of oxidative phosphorylation is important for understanding metabolic perturbations in disease evolution and cardiac protection.
disease evolution and cardiac protection.  +
Has editor [[Gnaiger E]]  +
Has info [https://www.ncbi.nlm.nih.gov/pubmed/28588260 PMID: 28588260] [http://rdcu.be/tgpY Sci Rep Open Access]  +
Has title Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840, DOI:10.1038/s41598-017-02789-8.  +
Instrument and method Oxygraph-2k  + , O2k-Protocol  +
Mammal and model Mouse  +
MiP area Respiration  + , Comparative MiP;environmental MiP  +
Pathways N  + , S  + , NS  +
Preparation Permeabilized tissue  +
Respiration and regulation Temperature  + , Cyt c  + , Threshold;excess capacity  + , Uncoupler  + , Inhibitor  +
Tissue and cell Heart  +
Was published by MiPNetLab AT Innsbruck Gnaiger E + , AT Innsbruck Oroboros + , CA Rimouski Blier PU + , CA Edmonton Lemieux H +
Was published in journal Sci Rep +
Was published in year 2017  +
Was written by Lemieux H + , Blier PU + , Gnaiger E +
Categories Publications
Modification date
"Modification date" is a predefined property that corresponds to the date of the last modification of a subject and is provided by Semantic MediaWiki.
13:51:32, 14 February 2019  +
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