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Mark 2012 Abstract Bioblast
Coupling states LEAK  + , ROUTINE  + , OXPHOS  +
Enzyme Complex IV;cytochrome c oxidase  + , TCA cycle and matrix dehydrogenases  +
Has abstract [[File:Mark Felix.jpg|right|150px|Felix Ma
[[File:Mark Felix.jpg|right|150px|Felix Mark]] Ongoing ocean warming and acidification have been found to particularly affect polar marine ecosystems. However, few data exist about the ability of Antarctic fish to respond to environmental change. While whole animal and transcriptomic data on the effects of ocean acidification and warming exist for fish, the acclimation capacities of the subcellular, organelle levels have been poorly studied in this respect. Diffusion of CO<sub>2</sub> into the bloodstream and into the intracellular milieu in aquatic water breathing organisms leads to an acidification of body fluids. Fish can regulate extracellular and intracellular pH by actively accumulating bicarbonate to compensate for the acidification of the extracellular and intracellular milieu. However, chronically elevated bicarbonate levels may interfere with a variety of metabolic processes. For example, bicarbonate is known to strongly interact with mitochondrial metabolism, among others it competitively inhibits citrate oxidation, ultimately influencing ET capacities. We therefore studied the capacities for mitochondrial acclimation towards elevated PCO<sub>2</sub> and bicarbonate levels in fish that were incubated at different water PCO<sub>2</sub>. We studied ET capacities in permeabilised heart fibres in fish-MiR06, modified to contain different levels of [HCO<sub>3</sub><sup>-</sup>], and enzymatic activies of citrate synthase and cytochrome c oxidase. We found a strong influence of bicarbonate on mitochondrial metabolism and a compensatory increase of mitochondrial capacities after hypercapnia acclimation. Our findings illustrate the importance of adjusting bicarbonate levels to represent intracellular conditions in fish and other aquatic organisms. # [ Simpson DP (1967) Regulation of renal citrate metabolism by bicarbonate ion and pH: observations in tissue slices and mitochondria. J Clin Invest 46: 225-238. Open Access] # [ Strobel A, Bennecke S, Leo E, Mintenbeck K, Poertner HO, Mark FC (2012) Metabolic shifts in the Antarctic fish ''Notothenia rossii'' in response to rising temperature and PCO<sub>2</sub>. Front Zool 9: 28. Open Access]
t;/sub>. Front Zool 9: 28. Open Access]  +
Has publicationkeywords Ocean acidification  + , Fish  + , Mitochondrial acclimation  +
Has title Mark FC, Strobel A, Poertner HO (2012) Ocean warming and acidification – when fish mitochondria turn sour. Mitochondr Physiol Network 17.12.  +
Instrument and method Oxygraph-2k  + , TIP2k  +
Mammal and model Fishes  +
MiP area Respiration  + , Comparative MiP;environmental MiP  +
Pathways N  + , S  + , CIV  + , NS  +
Preparation Permeabilized tissue  + , Isolated mitochondria  +
Respiration and regulation Oxygen kinetics  + , pH  + , Substrate  + , Temperature  +
Tissue and cell Heart  + , Liver  +
Was published by MiPNetLab DE Bremerhaven Mark FC +
Was submitted in year 2012  +
Was submitted to event Bioblast 2012 +
Was written by Mark FC + , Strobel A + , Poertner HO +
Categories Abstracts
Modification date
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09:14:08, 20 October 2017  +
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