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Miklikova 2018 Physiol Res
Additional label Labels  + , 2019-01  +
Coupling states LEAK  + , ROUTINE  + , ET  +
Has abstract Mesenchymal stem cells (MSCs) have been re
Mesenchymal stem cells (MSCs) have been reported to improve survival of cardiomyocytes (CMCs) and overall regeneration of cardiac tissue. Despite promising preclinical results, interactions of MSCs and CMCs, both direct and indirect, remain unclear. In this study, porcine bone marrow MSCs and freshly isolated porcine primary adult CMCs were used for non-contact co-culture experiments. Morphology, viability and functional parameters of CMCs were measured over time and compared between CMCs cultured alone and CMCs co-cultured with MSCs. In non-contact co-culture, MSCs improved survival of CMCs. CMCs co-cultured with MSCs maintained CMCs morphology and viability in significantly higher percentage than CMCs cultured alone. In viable CMCs, mitochondrial respiration was preserved in both CMCs cultured alone and in CMCs co-cultured with MSCs. Comparison of cellular contractility and calcium handling, measured in single CMCs, revealed no significant differences between viable CMCs from co-culture and CMCs cultured alone. In conclusion, non-contact co-culture of porcine MSCs and CMCs improved survival of CMCs with a sufficient preservation of functional and mitochondrial parameters.
f functional and mitochondrial parameters.  +
Has editor [[Plangger M]]  +
Has info [ PMID: 30607969 Open Access]  +
Has publicationkeywords Mesenchymal stem cells  + , Cardiomyocytes  + , Co-culture  + , Regenerative medicine  + , Mitochondria  +
Has title Miklíková M, Jarkovská D, Čedíková M, Švíg
Miklíková M, Jarkovská D, Čedíková M, Švíglerová J, Kuncová J, Nalos L, Kubíková T, Liška V, Holubová M, Lysák D, Králíčková M, Vištejnová L, Štengl M (2018) Beneficial effects of mesenchymal stem cells on adult porcine cardiomyocytes in non-contact co-culture. Physiol Res 67:619-31.
contact co-culture. Physiol Res 67:619-31.  +
Instrument and method Oxygraph-2k  +
Mammal and model Pig  +
MiP area Respiration  +
Preparation Intact cells  +
Tissue and cell Heart  +
Was published by MiPNetLab CZ Pilsen Kuncova J +
Was published in journal Physiol Res +
Was published in year 2018  +
Was written by Miklikova M + , Jarkovska D + , Cedikova M + , Sviglerova J + , Kuncova J + , Nalos L + , Kubikova T + , Liska V + , Holubova M + , Lysak D + , Kralickova M + , Vistejnova L + , Stengl M +
Categories Publications
Modification date
"Modification date" is a predefined property that corresponds to the date of the last modification of a subject and is provided by Semantic MediaWiki.
14:06:27, 10 January 2019  +
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