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Miller 2017 PLOS ONE
Additional label 2017-05  + , MitoFitPublication  +
Coupling states OXPHOS  + , ET  + , LEAK  +
Has abstract At the annual Iditarod Race, Alaskan Huski
At the annual Iditarod Race, Alaskan Huskies repeatedly run for up to 8 hours at 16 km/h to complete 1600 km. We previously demonstrated high rates of mitochondrial protein synthesis in Alaskan Huskies, which we suspected allowed rapid remodeling of mitochondrial proteins in response to energetic stress. The purpose of this study was to examine mitochondrial respiration in permeabilized skeletal muscle fibers of Alaskan Huskies in the offseason (Non-raced) and following the 1600 km Iditarod Sled Dog Race (Raced). We hypothesized that compared to Non-raced Huskies, raced Huskies that completed a 1600 km race would have greater mitochondrial respiratory capacities, and improvements in capacities of oxidative phosphorylation (OXPHOS) based on NADH-generating substrates as compared to fatty acids. Using high-resolution respirometry (HRR) we investigated the respiration of permeabilized muscle fibers from Alaskan Huskies. Maximum capacities were 254±26 pmol.s<sup>-1</sup>.mg<sup>-1</sup> for OXPHOS (coupled, P) and 254±37 pmol.s<sup>-1</sup>.mg<sup>-1</sup> for the electron transfer-pathway (ET-pathway; non-coupled, E). After racing respiratory capacities from NADH-linked substrates, but not fat-derived substrates increased. Finally, the OXPHOS to ET capacity ratio (P/E) increased after racing from 0.90±0.03 to 0.97±0.02. From our previous studies and the current study, we conclude that Alaskan Huskies maintain high mitochondrial protein turnover to facilitate rapid adaptation to environmental extremes and energetic challenges.
nmental extremes and energetic challenges.  +
Has editor [[Kandolf G]]  +
Has info [ PMID: 28445477 Open Access]  +
Has title Miller B, Hamilton K, Boushel R, Williamson K, Laner V, Gnaiger E, Davis M (2017) Mitochondrial respiration in highly aerobic canines in the non-raced state and after a 1600-km sled dog race. PLOS ONE 12:e0174874.  +
Instrument and method Oxygraph-2k  +
Mammal and model Dog  +
MiP area Respiration  + , Exercise physiology;nutrition;life style  +
Pathways F  + , N  + , S  + , NS  + , ROX  +
Preparation Permeabilized tissue  +
Tissue and cell Skeletal muscle  +
Was published by MiPNetLab AT Innsbruck Oroboros + , AT Innsbruck Gnaiger E + , US CO Fort Collins Miller BF + , CA Vancouver Boushel RC + , US OK Stillwater Davis MS + , US CO Fort Collins Hamilton K +
Was published in journal PLOS ONE +
Was published in year 2017  +
Was written by Miller B + , Hamilton K + , Boushel RC + , Williamson K + , Laner V + , Gnaiger E + , Davis MS +
Categories Publications
Modification date
"Modification date" is a predefined property that corresponds to the date of the last modification of a subject and is provided by Semantic MediaWiki.
08:17:46, 24 June 2019  +
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