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Rodrigues 2018 Sci Rep
Additional label Labels  + , 2018-08  +
Coupling states OXPHOS  +
Has abstract The xenotopic expression of the alternativ
The xenotopic expression of the alternative oxidase AOX from the tunicate ''Ciona intestinalis'' in diverse models of human disease partially alleviates the phenotypic effects of mitochondrial respiratory chain defects. AOX is a non-proton pumping, mitochondrial inner membrane-bound, single-subunit enzyme that can bypass electron transport through the cytochrome segment, providing an additional site for ubiquinone reoxidation and oxygen reduction upon respiratory chain overload. We set out to investigate whether AOX expression in ''Drosophila'' could counteract the effects of mitochondrial DNA (mtDNA) replication defects caused by disturbances in the mtDNA helicase or DNA polymerase γ. We observed that the developmental arrest imposed by either the expression of mutant forms of these enzymes or their knockdown was not rescued by AOX. Considering also the inability of AOX to ameliorate the phenotype of tko<sup>25t</sup>, a fly mutant with mitochondrial translation deficiency, we infer that this alternative enzyme may not be applicable to cases of mitochondrial gene expression defects. Finding the limitations of AOX applicability will help establish the parameters for the future putative use of this enzyme in gene therapies for human mitochondrial diseases.
herapies for human mitochondrial diseases.  +
Has editor [[Plangger M]]  + , [[Kandolf G]]  +
Has info [ PMID: 30022066 Open Access]  +
Has title Rodrigues APC, Camargo AF, Andjelkovic A, Jacobs HT, Oliveira MT (2018) Developmental arrest in ''Drosophila melanogaster'' caused by mitochondrial DNA replication defects cannot be rescued by the alternative oxidase. Sci Rep 8:10882.  +
Instrument and method Oxygraph-2k  +
Mammal and model Drosophila  +
MiP area Respiration  + , mtDNA;mt-genetics  + , Developmental biology  +
Pathways N  + , CIV  + , ROX  +
Preparation Homogenate  + , Oxidase;biochemical oxidation  +
Was published by MiPNetLab FI Helsinki Jacobs HT + , BR Jaboticabal Oliveira MT +
Was published in journal Sci Rep +
Was published in year 2018  +
Was written by Rodrigues APC + , Camargo AF + , Andjelkovic A + , Jacobs HT + , Oliveira MT +
Categories Publications
Modification date
"Modification date" is a predefined property that corresponds to the date of the last modification of a subject and is provided by Semantic MediaWiki.
09:02:01, 14 August 2018  +
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